A strain of pseudomonas degrading 3,4-benzopyrene, method and application
A technology of Pseudomonas and benzopyrene, which is applied in the fields of microbiology, biochemistry and fermentation engineering, can solve the problems of high technical difficulty, high cost, easy harm to human health and public environment, etc., and meets the requirements of low experimental conditions. , avoid costly effects
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0036] The isolation, purification and identification of the Pseudomonas of embodiment 1
[0037] The Pseudomonas was isolated from the soil of a coking plant in Donggang, Huangshi. The Pseudomonas was deposited on January 2, 2020 at China Type Culture Collection Center (preservation address: China. Wuhan. Wuhan University), its classification is named Pseudomonas sp., the deposit number is CCTCC M2020004. The Pseudomonas can utilize and degrade 3,4-benzopyrene.
[0038] (1) Isolation of the Pseudomonas
[0039] Take 50g of soil from a coking plant in Donggang, Huangshi, and place it in 100mL inorganic salt liquid culture for shaking culture to enrich the microorganisms in it. After one week of enrichment culture, take 5mL supernatant and transfer it into 50mL inorganic salt liquid medium with 3,4-benzopyrene as the single carbon source, wherein the concentration of 3,4-benzopyrene is 20mg / L, acclimation nourish. The culture solution was taken for subculture every seven...
Embodiment 23
[0044] Degradation and degradation rate of embodiment 23,4-benzopyrene
[0045] (1) Degradation and recovery of 3,4-benzopyrene
[0046] The medium for cultivating the Pseudomonas sp. is MSM liquid medium with 3,4-benzopyrene as the single carbon source. Components contained in each liter of MSM liquid medium: KH 2 PO 4 338.8mg, (NH 4 ) 2 SO 4 234.0mg,Na 2 CO 3 100.0mg, CaCl 2 3.9 mg, MgSO 4 ·7H 2 O 59.3 mg, Na 2 HPO 4 12H 2 O 890.7mg, FeSO 4 0.3mg, FeCl 2 4H 2 O 1.5mg, CoCl 2 ·6H 2 O 0.19mg, MnSO 4 ·7H 2 O 0.1mg, ZnCl 2 0.07mg,NiCl 2 ·6H 2 O0.024mg, NaMoO 4 2H 2 O 0.024mg, MnCl 2 4H 2 O 0.006mg, CuCl 2 2H 2 O 0.002 mg.
[0047] 1) The Pseudomonas was inoculated in a test tube containing 5 mL of liquid LB medium, and cultured overnight on a shaking table at 30° C. for activation.
[0048] 2) Centrifuge the cultured bacterial solution overnight at 8000rpm for 2min, discard the supernatant, resuspend with sterile water, centrifuge again at 8000...
Embodiment 3
[0057] Example 3 Exploration of the Degradation Ability of Pseudomonas BaP to Other Kinds of PAHs
[0058] The medium for cultivating the Pseudomonas sp. is to use benzene, carbazole, benzoanthracene, pyrene, benzofluoranthene, phenanthrene, dibenzoanthracene, fluoranthene, naphthalene or ethylbenzene as a single carbon source MSM liquid medium. Components contained in each liter of MSM liquid medium: KH 2 PO 4 338.8mg, (NH 4 ) 2 SO 4 234.0mg,Na 2 CO 3 100.0mg, CaCl 2 3.9 mg, MgSO 4 ·7H 2 O59.3mg, Na 2 HPO 4 12H 2 O 890.7mg, FeSO 4 0.3mg, FeCl2 4H 2 O 1.5mg, CoCl 2 ·6H 2 O 0.19mg, MnSO 4 ·7H 2 O 0.1mg, ZnCl 2 0.07mg,NiCl 2 ·6H 2 O 0.024mg, NaMoO 4 2H 2 O 0.024mg, MnCl 2 4H 2 O 0.006mg, CuCl 2 2H 2 O 0.002mg.
[0059] 1) The Pseudomonas was inoculated in a test tube containing 5 mL of liquid LB medium, and cultured overnight on a shaking table at 30° C. for activation.
[0060] 2) Centrifuge the cultured bacterial solution overnight at 8000rpm f...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More