Infrared multi-mode microscopic device based on liquid acousto-optic lens and microscopic method thereof

Abstract

The invention discloses an infrared multi-mode microscopic device based on a liquid acousto-optic lens and a microscopic method thereof. The device comprises a near-infrared reflection type confocal microscopic unit, a near-infrared reflection type photoacoustic microscopic unit, an intermediate infrared transmission type photoacoustic microscopic unit, a control unit (22) and a display unit (23).By the adoption of a method of the novel liquid acousto-optic lens, light waves and sound waves can have the same relative acoustic and optical refractive indexes through interfaces of two differentliquid with the same refractive index, and coaxial confocal configuration of optical illumination and acoustic detection is achieved. Compared with the prior art, the disclosed device and method havethe following advantages: imaging sensitivity detection is improved and thus multispectral imaging of near-infrared reflection type confocal microscopy, near-infrared reflection type photoacoustic microscopy and mid-infrared transmission type photoacoustic microscopy is realized, so that the targeting property of molecules is predicted, the signal-to-noise ratio is increased, the penetration depth is large; and comprehensive and complementary structure and function information is provided in the fields of biological sample tissue and cytology research.

Description

technical field [0001] The invention relates to the research field of biological tissue and cell microscopic imaging, in particular to an infrared multimodal microscopic device and a microscopic method thereof. Background technique [0002] In order to achieve the coaxial confocal configuration of optical path illumination and acoustic wave detection, optically resolved photoacoustic systems usually use a special combination of separate acoustic wave lenses and objective lenses, and use their reflected / transmitted acoustic waves and transmitted / reflected beams to achieve acoustic waves. and focusing of light waves, thus making the system bulky and complex. [0003] In recent years, laser confocal scanning imaging and laser photoacoustic microscopy have developed rapidly, but they are still limited to the visible light band (400nm-780nm), and are affected by the autofluorescence of biological sample tissues, resulting in a decrease in contrast; at the same time, biological sa...

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Problems solved by technology

[0004] Laser scanning confocal microscopy based on the principle of optical scattering contrast uses spatial filters (small holes at the detection end) to eliminate out-of-focus stray light, and uses photomultiplier tubes to collect diffraction-limited points of interest, thereby providing optical sectioning capabilities, which can The spatial resolution necessary to identify changes in biological tissues and cells; however, confocal laser microscopy makes it difficult to see deep structures in living tissues because light is highly dispersed and absorbed in biological tissues, which are often more than 1mm, insufficient observation of deeper tissues

[0005] Laser scanning photoacoustic imaging technology focuses the laser beam tightly on the biological tissue based on the photoacoustic effect. The light absorption in the biological tissue is converted into sound waves through thermoelastic expansion. The PVDF ultrasonic transducer collects the acoustic signal to form one-dimensional depth information, and then uses the platform Scanning and imaging three-dimensional information, photoacoustic imaging is only sensitive to light absorption, rich in optical contrast advantages, deep penetration depth and high spatial resolution, the endogenous tissue absorption of common organisms is hemoglobin, myoglobin, melanin, lipid and water; but it does not provide structural background information for the acquired images

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