Method for resolving optical isomers by electrodialysis technology

An optical isomer and electrodialysis technology, applied in the biological field, can solve the problems of recrystallization and purification, low yield and high cost, and achieve the effect of reducing production cost, improving yield and product quality

Pending Publication Date: 2020-09-04
GUANG AN MOJIA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is room for improvement in this process. For example, a large amount of organic solvents are used in the extraction and refining process of D-pantoate obtained by enzymatic reaction, which brings environmental and cost problems, and the crude product of D-pantoate needs to be extracted. Refined by recrystallization, low yield and high cost

Method used

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  • Method for resolving optical isomers by electrodialysis technology
  • Method for resolving optical isomers by electrodialysis technology
  • Method for resolving optical isomers by electrodialysis technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052]

[0053] 1. Preparation of enzyme conversion solution: 2L system, add 600g of racemic pantotolactone, add 300g of immobilized cells containing D-pantotolactone hydrolase, pH7.0&30℃, 200rpm mechanical stirring, 15N NH 3 .H 2 O titration maintains a pH value of 7.0 and reacts for 3 hours;

[0054] 2. Pretreatment of the enzyme conversion solution: first filter with filter cloth, then filter with a 0.2μm microfiltration membrane, and then filter with a 50KD ultrafiltration membrane;

[0055] 3. Electrodialysis separation: use homogeneous membrane membrane stack B (size: 10*30cm; number of membrane pairs: 5 pairs), pump the ultrafiltrate supernatant into the electrodialysis desalination chamber, put 2L pure water in the concentration chamber, adjust The flow rate makes the pressure of the three chambers equal, and the constant pressure is 10V until the conductivity of the desalination chamber is less than 100μs / cm;

[0056] Pump the clear liquid from the concentration ...

Embodiment 2

[0060]

[0061] 1. Preparation of enzyme conversion liquid: 3L system, add 900g of racemic pantotolactone, add 90g of cells containing D-pantotolactone hydrolase, pH7.0&30℃, 200rpm mechanical stirring, 15NNH 3 .H 2 O titration maintains a pH value of 7.0 and reacts for 5 hours;

[0062] 2. Pretreatment of the enzyme conversion solution: first centrifuge with a butterfly centrifuge, then filter with a 0.4μm microfiltration membrane, and then filter with a 20KD ultrafiltration membrane;

[0063] 3. Electrodialysis separation: use the heterogeneous membrane membrane stack Z (size: 10*20cm; number of membrane pairs: 10 pairs), pump the ultrafiltrate supernatant into the electrodialysis desalination chamber, put 3L pure water in the concentration chamber, adjust The flow rate makes the pressure of the concentrating chamber three times that of the desalination chamber, and operates at a constant pressure of 25V until the conductivity of the desalination chamber is <100μs / cm;

...

Embodiment 3

[0070]

[0071] 1. Preparation of enzyme conversion solution: 1L system, add 20ml 3-cyclohexene-1-methyl carboxylate, add 10g Novozyme435 lipase, pH7.5&35℃, 200rpm mechanical stirring, 1N NaOH titration to maintain the pH value of 7.5, react 5h;

[0072] 2. Pretreatment of the enzyme conversion solution: filter with filter paper first, and then filter with a 0.4 μm microfiltration membrane;

[0073] 3. Electrodialysis separation: use homogeneous membrane membrane stack S (size: 10*20cm; number of membrane pairs: 10 pairs), pump the ultrafiltrate supernatant into the electrodialysis desalination chamber, put 1L pure water in the concentration chamber, adjust The flow rate makes the pressure of the three chambers equal, and the constant pressure is 14V until the conductivity of the desalination chamber drops to <100μs / cm;

[0074] Pump the clear liquid from the concentration chamber into the desalination chamber of the second-stage electrodialysis device, put 1L of pure wate...

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Abstract

The invention provides a method for resolving optical isomers from racemates by an electrodialysis technology. Specifically, the electrodialysis technology is applied to an enzyme resolution process,and is mainly applied to separation of products after enzyme resolution. Taking a D-pantoic acid lactone preparation process as an example, a key point lies in that D-pantoic acid and L-pantoic acid lactone are separated from an enzyme resolution solution through an electrodialysis method, an existing organic solvent extraction method is replaced, the process method is simple and easy to implement, the yield of D-pantoic acid is high, the purity of D-pantoic acid is good, the usage amount of an organic solvent is greatly reduced, the production cost is reduced, the method is environmentally friendly, the working environment of workers can be improved to a great extent, and the operation safety index is improved. The method provided by the invention has great application potential in the enzyme resolution and separation process.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the separation of optical isomers from racemates by using biocatalysis technology and electrodialysis technology. Background technique [0002] Chirality is an essential attribute of nature, and many biomacromolecules and bioactive substances have chiral characteristics. Although two or more different configurations of chiral substances have exactly the same chemical composition, their physiological activities are often different. Usually, only one configuration has the desired activity, and the other configurations have little or no effect, and may even have toxic side effects . For example, pantothenic acid, also known as pantothenic acid, is one of the B vitamins and a component of coenzyme A. It participates in the metabolism of protein, fat, and sugar, and plays an important role in substance metabolism. Its active ingredient is D-configuration D-pantothenic acid (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P41/00
CPCC12P41/001C12P41/003C12P41/005C12P17/04C12P7/40C12P7/42B01D61/44B01D61/58B01D61/145B01D61/147B01D2311/18B01D2311/2643B01D2311/2676B01D2311/04
Inventor 邱贵森苏金环曾聪明蒋泰隆陈彦刘文杰
Owner GUANG AN MOJIA BIOTECHNOLOGY CO LTD
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