Method for remedying heavy metal polluted soil by inducing calcium carbonate precipitation through microorganisms

A technology for heavy metal-contaminated soil and soil repair, which is applied in the field of microorganism-induced calcium carbonate precipitation to repair soil heavy metal pollution, and achieves the effect of wide source of materials, abundant resources and strong applicability

Active Publication Date: 2020-09-18
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for microorganism-induced calcium carbonate precipitation to repair soil heavy metal pollution, to solve the above-mentioned problems in repairing heavy metal-contaminated sites, and the operation is simple, the cost is low, the repair effect is uniform, and the strength of the repaired soil is high. high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, a kind of microorganism-induced calcium carbonate precipitation of the present invention repairs the method for soil heavy metal pollution, comprises the following steps:

[0038] Step 1, the production of bacterial liquid and cementing liquid

[0039] Step 1.1, using the formula ATCC 1376NH recommended by the American Culture Collection 4 -YE prepare the culture solution, then sterilize the culture solution at 121-123°C for 20-30 minutes, then place it on the ultra-clean workbench for ultraviolet sterilization and ventilation, and wait until the temperature of the culture solution drops to room temperature for use. In this example, the culture solution was sterilized at 121° C. for 30 minutes.

[0040] In the present example, the American Culture Collection recommended formula ATCC 1376NH 4 The composition of the culture medium configured by -YE is: 20g yeast extract, 10g (NH 4 ) 2 SO 4 , 0.13mol / L Tris Buffer, add deionized water to 1L, and adjust t...

Embodiment 2

[0064] Embodiment 2: to repair Pb 2+ Take polluted soil as an example

[0065] Step 1, the production of bacterial liquid and cementing liquid

[0066] Step 1.1, configure culture medium: 20g yeast extract, 10g (NH 4 ) 2 SO 4 , 0.13mol / L Tris Buffer, add deionized water to 1L, adjust the pH of the culture solution to 9.0 with 1mol / L HCl, sterilize at 121°C for 30min, put it on a clean bench for ultraviolet sterilization and ventilate, wait When the temperature of the culture medium was lowered to room temperature, it was ready for use.

[0067] Step 1.2, after the temperature of the culture solution drops to room temperature, take the culture solution in a container, use a sterile pipette to add Bacillus Pasteurella to the culture solution to obtain a bacterial solution, and the volume ratio of Bacillus Pasteurella to the culture solution is The ratio is 1:100, put the bacterial liquid into the incubator for cultivation, the temperature of the incubator is set at 30°C, th...

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Abstract

The invention discloses a method for remedying heavy metal polluted soil by inducing calcium carbonate precipitation through microorganisms, and belongs to the technical field of geotechnical engineering. The method comprises the following steps: step 1, preparing a bacterial liquid and a cementing liquid; step 2, detecting the concentration of heavy metal pollutants in a suspected contaminated site; step 3, remedying the heavy metal polluted soil; and step 4, detecting the remedying effect. A slurry mixing mode is adopted, the cementing liquid, the bacterial liquid and the heavy metal pollutants can be in full contact, the operation is easy, the cost is low, the remediation uniformity is good, secondary pollution is avoided, and remedied soil can serve as a roadbed filler.

Description

technical field [0001] The invention belongs to the technical field of geotechnical engineering, and relates to a method for remediating heavy metal pollution in soil by inducing calcium carbonate precipitation by microorganisms. Specifically, it is a bioremediation method that uses bacteria to treat heavy metal-contaminated soil. Background technique [0002] At present, soil pollution has become one of the important global environmental problems. In the past 20 years, with the rapid development of society and economy, the process of industrialization has been accelerated and human activities have been intensive, the polluted area of ​​soil in my country has continued to expand, and the soil quality has continued to deteriorate. As a common pollutant, heavy metal pollution will have adverse effects on the environment after polluting water and soil, causing serious harm to the human body, affecting the engineering properties of the soil, and leading to engineering accidents. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10
Inventor 康博邓伟豪许龙查甫生刘从民
Owner HEFEI UNIV OF TECH
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