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Decomposition promoting and nitrogen promoting microbial inoculum for composting of excrement and urine of livestock and poultry and preparation and application method

A technology of colony and degrading bacteria, applied in the field of microorganisms, to achieve the effects of avoiding ammonia volatilization, strong high temperature tolerance, and improving sulfur nutrition

Active Publication Date: 2020-09-18
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology often directly uses different functional strains with only a single degradation effect, and uses various synthetic media to expand and mix them under artificial conditions and directly use them as compost bacteria agents, which cannot really solve the above problems

Method used

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  • Decomposition promoting and nitrogen promoting microbial inoculum for composting of excrement and urine of livestock and poultry and preparation and application method
  • Decomposition promoting and nitrogen promoting microbial inoculum for composting of excrement and urine of livestock and poultry and preparation and application method
  • Decomposition promoting and nitrogen promoting microbial inoculum for composting of excrement and urine of livestock and poultry and preparation and application method

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0032] The preparation method of the solid preparation of the microorganism is:

[0033] (1) Obtaining each microbial seed solution: For bacteria (i.e. other microorganisms except Thermoascus aurantiacus ZJ3), pick an inoculation loop thalli respectively, and cultivate until the concentration of viable bacteria is 10 9 CFU / mL, the seed solution was prepared. For the fungus (T. aurantiacus ZJ3), a loop mycelium was picked from T. aurantiacus, and cultured to a mycelium concentration of 15%, to obtain a seed liquid.

[0034] (2) Insert each microbial seed liquid obtained in step (1) into the livestock and poultry manure fermentation medium separately at a weight ratio of 1:5, adjust the water content between 50% and 60%, and place it in a solid fermenter Under the temperature conditions adapted to each strain, domestication and expansion are carried out until the number of bacteria or spores in a single gram of sample is ≥10 9 That's it. After the expansion is completed, the ...

Embodiment 1

[0037] Embodiment one: preliminary screening of each microorganism in the microbial inoculum

[0038] Livestock and poultry manure is a common raw material for composting. Its main components are carbohydrates (mainly cellulose), crude protein and inorganic salts. During the composting process, auxiliary materials mainly composed of cellulose are often added. Whether the main components in livestock and poultry manure can be efficiently used for rapid growth and reproduction, and whether the main components can be efficiently converted into humus is the key to the selection of livestock and poultry manure-specific functional composting microorganisms. Therefore, in this example, a mixture of common livestock and poultry manure (chicken manure and pig manure) was used as the only carbon source and nitrogen source to screen related microorganisms. In addition, due to the large temperature difference in different stages of composting, in order to avoid devastating damage to some ...

Embodiment 2

[0119] Embodiment two: the secondary screening of each microorganism in microbial bacterial agent

[0120] Embodiment 1 When selecting microorganisms, some microorganisms have multiple strains that can better achieve the purpose of the invention. On the basis of Example 1, antagonism tests among microorganisms were supplemented to finally determine the types of microorganisms used in the microbial inoculum. Alternative microorganisms for antagonistic tests are shown in Table 9.

[0121] Table 9 carries out the microorganism list of antagonistic test

[0122]

[0123]

[0124] Each microorganism in Table 9 was activated and prepared as microbial seed liquid. Then use the appropriate temperature range to group: specifically divided into 20~45℃ group (including N10, L3A, H0, 1.10823, DS-8, DS-4) and 45~60℃ group (including 50-11, ZJ3, GZY6- 1. SD2-2).

[0125] The seed liquids of each microorganism in the same group were sucked and spread on the solid culture medium (pl...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to decomposition promoting and nitrogen promoting microbial inoculum for composting of excrement and urine of livestock and poultry and a preparation and application method. The decomposition promoting and nitrogen promoting microbial inoculum adopts the technical scheme that the decomposition promoting and nitrogen promoting microbial inoculum is bacillus brevis, the decomposition promoting and nitrogen promoting microbial inoculum is preserved in China General Microbiological Culture Collection Center at27 March, 2020, and the preservation No. is CGMCC No.19516. A microorganism composition prepared from the bacillus brevis comprises protein degradation bacteria, cellulose degradation bacteria, directional decomposition promoting microorganisms, keratin degrading bacteria, lactic acid bacillus, ammonia oxidizing bacteria and sulphur-oxidising bacteria. The protein degradation bacteria comprise bacillus subtilis. The microorganism composition can treat livestock and poultry excrement and urine pointedly, the mineralization rate during composting of the livestock and poultry excrement and urinecan be reduced, the humification rate can be increased, volatilization of ammonia and foul gas can be avoided, and the composting product quality can be improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a rot-promoting nitrogen-containing and deodorizing bacterial agent for composting and a preparation and application method thereof. Background technique [0002] Composting refers to the biochemical process in which degradable organic matter is transformed into inorganic matter or humus with relatively stable properties through mineralization and humification of compost raw materials under the condition of microorganism participation. [0003] Composting control includes two aspects of process control and pollution control. Among them, composting process control technology is an important technology to reduce operating costs and improve the quality of composting products. Reducing the proportion of mineralization rate of compost raw materials and increasing the proportion of humification are the main signs to achieve compost control and distinguish it from na...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F17/20C12R1/08C12R1/07C12R1/01C12R1/645
CPCC12N1/20C12N1/14C05F17/20C12R2001/08C12N1/205Y02W30/40
Inventor 李东陈意超
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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