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Glassy carbon electrode modified by poly L-cysteine and reduced graphene oxide loaded nano-silver and application thereof

A glassy carbon electrode, cysteine ​​technology, which is applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, and can solve the problems of prone to false positive results, small scope of application, and difficulty.

Active Publication Date: 2020-09-25
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Instrumental analysis methods, such as chromatography, have good separation effect and high sensitivity, but the pretreatment of samples is more complicated, and the requirements for instruments are higher, and the price of instruments is relatively expensive, and the scope of application is small
However, immunoassay methods, such as enzyme-linked immunoassay, have low repeatability, are prone to false positive results, and the preparation of antibodies is also more complicated and difficult.

Method used

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  • Glassy carbon electrode modified by poly L-cysteine and reduced graphene oxide loaded nano-silver and application thereof
  • Glassy carbon electrode modified by poly L-cysteine and reduced graphene oxide loaded nano-silver and application thereof
  • Glassy carbon electrode modified by poly L-cysteine and reduced graphene oxide loaded nano-silver and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of poly-L-cysteine ​​and reduced graphene oxide supported nano-silver modified glassy carbon electrode, comprising the following steps:

[0041] Step 1, in 20mL 2mg / mL graphene oxide solution, add 100mg L-cysteine, 100mgAgNO 3 The powder and 400 μL of ammonia water with a mass percentage of 25% were left to stand in a water bath at 90°C for 10 hours to obtain reduced graphene oxide-loaded nano-silver, which was washed with water and then dried;

[0042] Step 2, disperse the reduced graphene oxide-loaded nano-silver treated in step 1 in 50mL of aqueous solution, and obtain a reduced graphene oxide-loaded nano-silver solution through ultrasonication until the dispersion is uniform;

[0043] Step 3: Grinding and polishing the bare glassy carbon electrode on 0.05 μm alumina powder, then ultrasonically treating it in absolute ethanol and water for 1 min, then rinsing with water and drying in the air;

[0044] Step 4, take 10 μL of the reduced graphene ...

Embodiment 2

[0046] Embodiment 2 Modifies the evaluation of electrode performance:

[0047] In order to explore the influence of each material on the electrode response, the cyclic voltammetry was used to study and compare the GCE, rGO-AgNPs / GCE, poly(L-Cys) / GCE and poly(L-Cys) / rGO-AgNPs / GCE The electrode was in potassium ferricyanide solution (1mmol L -1 ) in the electrochemical response signal. Such as figure 1 As shown, these four modified electrodes all have a pair of good redox peaks in potassium ferricyanide solution. On GCE, the potential difference between the redox peaks is less than 90 mV, indicating that the electrode has been activated. Compared with GCE, the peak current of rGO-AgNPs / GCE decreased, and the peak potential difference became wider; when poly(L-Cys) was modified on GCE, the peak potential difference was similar to that of GCE, but the peak current was significantly increased; When poly(L-Cys) and rGO-AgNPs are co-modified on GCE, the peak potential difference ...

Embodiment 3

[0049] Embodiment 3 modified electrode detects to chloramphenicol:

[0050] The electrochemical sensors of GCE, rGO-AgNPs / GCE, poly(L-Cys) / GCE and poly(L-Cys) / rGO-AgNPs / GCE were investigated by cyclic voltammetry (CV). The response signal of chloramphenicol and explore how the detection signal of chloramphenicol is improved. Such as image 3 As shown, chloramphenicol was at -0.763V on GCE (Ep 1 ) and -0.432V (Ep 2 ) has two weak reduction peaks, of which the first reduction peak is very weak; Ep on rGO-AgNPs / GCE 1 The peak current was significantly enhanced, but a certain degree of negative shift occurred, while the Ep 2 No significant increase in peak current; Ep on poly(L-Cys) / GCE 2 The peak current is significantly enhanced, and the peak shape is also more beautiful, Ep 1 Although the peak current is enhanced, the peak shape becomes smooth and unsightly, and the peak potential also shifts negatively; when poly(L-Cys) and rGO-AgNPs are combined on GCE, the two reductio...

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Abstract

The invention belongs to the technical field of detection of chloramphenicol residues in animal food, and discloses a glassy carbon electrode modified by poly L-cysteine and reduced graphene oxide loaded nano-silver and a method for detecting chloramphenicol by using the electrode. The electrode is prepared through the following method which comprises the steps: carrying out ultrasonic treatment on reduced graphene oxide loaded nano-silver until the reduced graphene oxide loaded nano-silver is uniformly dispersed to obtain a reduced graphene oxide loaded nano-silver solution; dropwise coatingthe solution on the surface of a treated bare glassy carbon electrode, performing drying, putting the obtained reduced graphene oxide loaded nano-silver modified glassy carbon electrode into an L-cysteine aqueous solution, and carrying out cyclic voltammetry scanning polymerization, so as to obtain the poly L-cysteine and reduced graphene oxide loaded nano-silver modified glassy carbon electrode.The electrode can improve a chloramphenicol response signal, improve chloramphenicol detection reliability and realize qualitative or quantitative detection of chloramphenicol residual quantity in animal food.

Description

technical field [0001] The invention relates to the technical field of detection of chloramphenicol residues in animal food, in particular to a poly-L-cysteine ​​and reduced graphene oxide loaded nano-silver modified glassy carbon electrode and a method for detecting chloramphenicol using the electrode. Background technique [0002] Chloramphenicol (Chloramphenicol, CAP), as a synthetic bacteriostatic broad-spectrum antibiotic (below the structure diagram), has a good antibacterial effect on most Gram-positive bacteria and negative bacteria, and the price is low. Therefore, it has been widely used in the treatment and control of livestock and poultry diseases and various animal infectious diseases. my country also began to use it in veterinary clinics in the 1990s and added it to animal feed. In recent years, after people's exploration and research, it has been found that chloramphenicol has serious damage to the human hematopoietic system, which will cause the human body to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48B82Y30/00
CPCB82Y30/00G01N27/308G01N27/48
Inventor 毛烨炫张西亚刘亮李倩李家寅
Owner HENAN AGRICULTURAL UNIVERSITY
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