Artificial cornea and preparation method thereof
An artificial cornea and a combined technology, applied in prostheses, eye implants, tissue regeneration, etc., can solve the problems of increasing oxygen and nutrient exchange, low oxygen permeability of artificial cornea, high melting rate of corneal stroma, etc., to increase oxygen Exchange, improve mechanical strength, reduce the effect of melting
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Embodiment 1
[0024] The mold used is attached figure 1 , configure a HEMA aqueous solution with a mass fraction of 15%, add 5% ethylene glycol dimethacrylate (EDMA) crosslinking agent, add 1% initiator ammonium persulfate (APS), mix well and add to mold A , cover the B mold, and react at room temperature for 24 hours. After the reaction finishes, the B mold is removed, and the mixed solution of HEMA / MMA is added, wherein the mass fraction of HEMA is 50%, the crosslinking agent is 5% EDMA, and the initiator is 2% azobisisobutyronitrile (AIBN), Cover mold C and heat to 60°C for 24 hours. Wherein mold B has 12 circular columns at the position corresponding to the skirt, with a diameter of 1mm, and the skirt of the obtained artificial cornea has 12 macroscopic holes with a diameter of 1mm. The schematic diagram of the obtained artificial cornea is as follows figure 2 shown.
Embodiment 2
[0026] The implementation method is attached figure 1 As shown, configure a HEMA aqueous solution with a mass fraction of 35%, add 10% ethylene glycol dimethacrylate (EDMA) crosslinking agent, add 0.5% azobisisobutyronitrile (AIBN), mix well and add In mold A, cover mold B and heat to 70°C for 24 hours. After the reaction, remove the mold B, add a mixed solution of HEMA / MMA, wherein the mass fraction of HEMA is 65%, the crosslinking agent is 1% EDMA, the initiator is 1% tert-butyl alcohol peroxide, and cover the mold C , and reacted at room temperature for 24 hours. Wherein mold B has 3 annular pillars at the position corresponding to the skirt, the width is 1mm, and the skirt of the obtained artificial cornea promptly has 3 macro grooves with a width of 1mm, and its structure is as attached image 3 shown.
Embodiment 3
[0028] The number of grooves and / or holes in the skirt bracket has been screened by experiments. The larger the number and the larger the size, the more oxygen and nutrients will be exchanged, but too large holes / slots will affect the mechanical strength of the skirt. Therefore, The design of the groove / hole should be based on the normal mechanical support that does not affect the artificial cornea. Therefore, skirts of different sizes were prepared by the method of Example 1 or Example 2, and the experimental results of mechanical strength testing are shown in Table 1. .
[0029] Table 1 Comparison table of tensile strength of skirt with different pore size, hole diameter and number of holes
[0030]
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