Uric acid test kit capable of eliminating drug interference of calcium dobesilate and etamsylate in serum and preparation method thereof
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A technology of calcium dobesilate and ethylamine sulfonate, which is applied in the field of medical testing, can solve problems such as negative deviation, misjudgment of doctor's diagnosis and treatment, consumption of uric acid, etc., to achieve the elimination of negative interference, precise medication and treatment, and accuracy high effect
Active Publication Date: 2020-10-02
ZHONGSHAN BGH BIOTECH CO LTD
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Calcium dobesilate is used as a commonly used drug for microvascular protection and treatment, and fensulfate is used as a commonly used drug for hemostasis. These drugs are all strong reducing agents. When patients taking these drugs measure plasma uric acid levels, these reducing drugs will H produced by the decomposition of uric acid by uricase 2 o 2 direct reaction, consumes H 2 o 2 Cause serious negative deviation of uric acid value, resulting in misjudgment of doctors in diagnosis and treatment
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Embodiment 1
[0063] Reagent R1 components:
[0064] Tris buffer 50mM PH8.0;
[0065] Peroxidase 9 ku / L;
[0066] Ascorbate Oxidase 6 ku / L;
[0067] Vanadium pentoxide 1 mM;
[0068] 4-AAP 3 mM;
[0069] TRITON X-100 0.2%;
[0070] Borax 1%
[0071] Reagent R2 components:
[0072] Tris buffer 50 mM PH8.0
[0073] Uricase 9 ku / L
[0074] Bovine Serum Albumin 0.1%
[0075] TOOS 10 mM
[0076] Borax 1.0%
[0077] TRITON 0.1%.
Embodiment 2
[0079] Reagent R1 components:
[0080] HEPES buffer 50mM PH8.0
[0081] Peroxidase 7.2 ku / L
[0082] Ascorbate Oxidase 6.4 ku / L
[0083] Sodium ferrate 1 mM
[0084] 4-AAP 3 mM
[0085] TRITON X-100 0.2%
[0086] Gentamicin Sulfate 0.1%;
[0087] Reagent R2 components:
[0088] HEPES buffer 50 mM PH8.0
[0089] Uricase 11.2 ku / L
[0090] Bovine Serum Albumin 0.1%
[0091] TOOS 10 mM
[0092] Gentamicin Sulfate 0.1%
[0093] TRITON 0.1%.
Embodiment 3
[0095] Reagent R1 components:
[0096] Phosphate Buffer 50mM PH8.0
[0097] Peroxidase 4.6 ku / L
[0098] Ascorbate Oxidase 10 ku / L
[0099] Sodium metavanadate 2 mM
[0100] 4-AAP 3mM
[0101] TRITON X-100 0.2%
[0102] Sodium azide 0.1%;
[0103] Reagent R2 components:
[0104] Phosphate Buffer 50mM PH8.0
[0105] Uricase 20 ku / L
[0106] Bovine Serum Albumin 0.1%
[0107] TOOS 10mM
[0108] Sodium azide 0.1%
[0109] TRITON X-100 0.1%.
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Abstract
The invention discloses a uric acid test kit capable of eliminating drug interference of calcium dobesilate and etamsylate in serum and a preparation method thereof. According to the technical point,the test kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises a buffer solution, peroxidase, ascorbic acid oxidase, a preservative, a Trinder's reactant A, metallate or a nonionic surfactant; and the reagent R2 comprises a buffer solution, serum albumin, uricase, a preservative and a Trinder's reactant B or a nonionic surfactant. According to the uric acid test kit disclosed by the invention, one or more than one of high-redox-potential oxidized metallate, vanadium pentoxide and vanadium oxychloride is / are added into R1 to be mixed, and calcium dobesilate and etamsylate are oxidized by utilizing the metallate, H2O2 generated by uricase in decomposing uric acid after R2 is added is prevented from being consumed, and an accurate uric acid measured value is rapidly obtained.
Description
technical field [0001] The invention belongs to the technical field of medical examination, and relates to a uric acid test kit capable of eliminating the interference of calcium dobesilate and phenetamine drugs in serum and a preparation method thereof. Background technique [0002] Uric acid (Uric Acid, UA) is the final product of the metabolism of purine compounds in the body, which is weakly acidic. Uric acid can come from the body or from the catabolism of purines in food. In the body, uric acid is mainly produced in the liver, a small part can be excreted through the liver with bile, and most of the rest are excreted from the kidneys. The solubility of uric acid is small, so it is easy to precipitate crystals when the concentration in the body is high. Uric acid is considered to be closely related to the occurrence and development of gout, cardiovascular and cerebrovascular diseases, metabolic syndrome, ureteral stones and kidney diseases. In recent years, with the ...
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