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Clostridium butyricum freeze-dried powder as well as preparation method and application thereof

A clostridia, freeze-dried powder technology, applied in the directions of biochemical equipment and methods, freeze-dried transportation, microorganism-based methods, etc., to achieve the effect of strong freeze resistance and prolonged storage time

Pending Publication Date: 2020-10-09
科兴生物制药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to improve the spore rate of Clostridium butyricum and the viable count of freeze-dried powder has always been a problem

Method used

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  • Clostridium butyricum freeze-dried powder as well as preparation method and application thereof
  • Clostridium butyricum freeze-dried powder as well as preparation method and application thereof

Examples

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preparation example Construction

[0046] In addition, the applicant unexpectedly found that when magnesium sulfate and manganese sulfate exceed this amount, the formation of bacterial spores will be inhibited. The second aspect of the present invention provides a kind of preparation method of described Clostridium butyricum lyophilized powder, it comprises the following steps:

[0047] (1) Strain cultivation: Cultivate Clostridium butyricum to the stationary phase, the spore rate is greater than 90% and the spore shedding rate is greater than 10%, to obtain the Clostridium butyricum fermentation broth;

[0048] (2) collecting thallus: precipitating the fermentation liquid of Clostridium butyricum to obtain wet sludge of Clostridium butyricum;

[0049] (3) Adding a protective agent: mixing the Clostridium butyricum wet bacteria slime and the protective agent to obtain a mixture;

[0050] (4) Vacuum freeze-drying: equilibrate the mixture at 25-37°C for 20-60 minutes, pre-freeze at -40°C for 6-12 hours, and then...

Embodiment 1

[0086] Embodiment 1 of the present invention provides a Clostridium butyricum freeze-dried powder, the preparation raw material of which comprises Clostridium butyricum wet sludge and a protective agent, the weight ratio of which is 2:1.

[0087] The protective agent is skimmed milk powder and trehalose, and the weight ratio is 3:1.

[0088] The preparation method of the clostridium butyricum freeze-dried powder is specifically the following steps:

[0089] (1) Strain activation: In a sterile room, dissolve the low-temperature-preserved Clostridium butyricum strain in 25 mL of sterile saline, shake on a vortex shaker for 4 minutes, then shake in a water bath at 80°C for 15 minutes, shake The frequency is 120r / min to obtain the strain mixture;

[0090] (2) Expanded cultivation: inoculate the strain mixture in the first-grade seed culture medium according to the inoculation amount of 4% by volume, anaerobically cultivate it at 37°C for 12 hours, and stop the cultivation when th...

Embodiment 2

[0101] Example 2 of the present invention provides a Clostridium butyricum freeze-dried powder, the preparation raw material of which comprises Clostridium butyricum wet sludge and a protective agent, the weight ratio of which is 4:1.

[0102] The protective agent is skimmed milk powder and trehalose, and the weight ratio is 8:1.

[0103] The preparation method of the clostridium butyricum freeze-dried powder is specifically the following steps:

[0104] (1) Strain activation: In a sterile room, dissolve the low-temperature-preserved Clostridium butyricum strain in 25 mL of sterile saline, shake on a vortex shaker for 4 minutes, then shake in a water bath at 80°C for 15 minutes, shake The frequency is 120r / min to obtain the strain mixture;

[0105] (2) Expanded cultivation: inoculate the strain mixture in the first-grade seed culture medium according to the inoculation amount of 4% by volume, anaerobically cultivate it at 37°C for 12 hours, and stop the cultivation when the p...

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Abstract

The invention relates to the technical field of biological products. More specifically, the invention relates to clostridium butyricum freeze-dried powder as well as a preparation method and application thereof. The spore rate of the clostridium butyricum freeze-dried powder is greater than 90%, and the viable count in the freeze-dried powder is 2.0*10<10> cfu / g to 3.0*10<10> cfu / g. The clostridium butyricum freeze-dried powder provided by the invention is relatively strong in freezing resistance, the viable count in the freeze-dried powder is greater than 2.0*10<10> cfu / g, the spore rate is greater than 90%, the preservation time of the clostridium butyricum freeze-dried powder is prolonged, and later product processing, storage and transportation are facilitated.

Description

technical field [0001] The invention relates to the technical field of biological products, more specifically, the invention relates to a lyophilized powder of Clostridium butyricum, a preparation method and an application thereof. Background technique [0002] In recent years, probiotics have been widely used in the fields of functional dairy products, health food, microecological preparations, and medical treatment. Specifically, they refer to a class of active microorganisms that have functional health benefits for the host. They can regulate human immune function, improve intestinal It has the advantages of micro-ecological balance of flora, reduction of blood fat and cholesterol, and promotion of digestion and absorption of nutrients. Common probiotics include lactic acid bacteria, bifidobacteria, Clostridium butyricum and yeast. Clostridium butyricum, as the most important physiological bacterium in the intestinal tract of the body, is closely related to the growth an...

Claims

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Application Information

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IPC IPC(8): A61K35/742A61K9/19A61K47/46A61K47/26A61P1/12A61P1/00A61P29/00A61P35/00A61P1/16A23L33/135C12N1/20C12R1/145
CPCA61K9/19A61K35/742A61K47/26A61K47/46A23L33/135A61P1/00A61P1/12A61P1/16A61P29/00A61P35/00C12N1/20
Inventor 马玉涛李国强崔宁韩登兰耿宏伟崔兆臣
Owner 科兴生物制药股份有限公司
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