Polydopamine-doped glucan hydrogel porous scaffold as well as preparation method and application thereof

A technology of porous scaffolds and dextran water, applied in biochemical equipment and methods, cell culture supports/coatings, bone/connective tissue cells, etc. Application and other issues, to achieve the effect of enhancing adhesive performance, enhancing mechanical properties, and good degradability

Pending Publication Date: 2020-10-09
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dextran has good biocompatibility and degradability, but the adhesion ability of dextran gel is weak, an

Method used

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  • Polydopamine-doped glucan hydrogel porous scaffold as well as preparation method and application thereof
  • Polydopamine-doped glucan hydrogel porous scaffold as well as preparation method and application thereof
  • Polydopamine-doped glucan hydrogel porous scaffold as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of dextran / polydopamine porous hydrogel cell scaffold

[0020] Its preparation method steps are as follows:

[0021] 1) The aqueous solution of dopamine hydrochloride and sodium hydroxide is prepared by dissolving 30 g of dopamine hydrochloride solid in 1 L of 1.2 M sodium hydroxide aqueous solution, and performing an oxidation reaction under air conditions (25° C., magnetic stirring for 24 h);

[0022] 2) Sodium hydroxide solution (1.2M) was used to dissolve dextran to prepare a dextran solution with a concentration of 15% (w / v);

[0023] 3) Take 2mL of the dopamine hydrochloride sodium hydroxide aqueous solution in step 1, 5mL of the dextran solution in step 2 and 3mL of 1.2M sodium hydroxide solution and mix together, and stir evenly at room temperature.

[0024] 4) Add 1.0 mL of cross-linking agent EGDE to the mixed solution prepared in step (3), stir evenly, place in a 37°C incubator, and cross-link for 12 hours to form a gel, which is recor...

Embodiment 2

[0025] Example 2: Preparation of dextran / polydopamine porous hydrogel cell scaffold

[0026] Its preparation method steps are as follows:

[0027] 1) The aqueous solution of dopamine hydrochloride and sodium hydroxide is prepared by dissolving 30 g of dopamine hydrochloride solid in 1 L of 1.2 M sodium hydroxide aqueous solution, and performing an oxidation reaction under air conditions (25° C., magnetic stirring for 24 h);

[0028] 2) Sodium hydroxide solution (1.2M) was used to dissolve dextran to prepare a dextran solution with a concentration of 15% (w / v);

[0029] 3) Take 3 mL of the dopamine hydrochloride sodium hydroxide aqueous solution in step 1, 5 mL of the dextran solution in step 2 and 2 mL of 1.2 M sodium hydroxide solution and mix together, and stir evenly at room temperature.

[0030] 4) Add 1.0 mL of cross-linking agent EGDE to the mixed solution prepared in step (3), stir evenly, place in a 37°C incubator, and cross-link for 12 hours to form a gel, which is r...

Embodiment 3

[0031] Example 3: Preparation of dextran / polydopamine porous hydrogel cell scaffold

[0032] Its preparation method steps are as follows:

[0033] 1) The aqueous solution of dopamine hydrochloride and sodium hydroxide is prepared by dissolving 30 g of dopamine hydrochloride solid in 1 L of 1.2 M sodium hydroxide aqueous solution, and performing an oxidation reaction under air conditions (25° C., magnetic stirring for 24 h);

[0034] 2) Sodium hydroxide solution (1.2M) was used to dissolve dextran to prepare a dextran solution with a concentration of 15% (w / v);

[0035] 3) Take 5 mL of the dopamine hydrochloride sodium hydroxide aqueous solution in step 1 and 5 mL of the dextran solution in step 2 and mix together, and stir at room temperature evenly.

[0036] 4) Add 1.0 mL of cross-linking agent EGDE to the mixed solution prepared in step (3), stir evenly, place in a 37°C incubator, and cross-link for 12 hours to form a gel, which is recorded as D4.

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Abstract

The invention relates to a polydopamine doped glucan hydrogel porous scaffold as well as a preparation method and application thereof. The method is used for cell in-vitro 3D culture and wound repair.The hydrogel scaffold is prepared by the steps as follows: preparing the hydrogel scaffold; dissolving glucan by using a sodium hydroxide solution to produce a glucan solution; adding dopamine hydrochloride into a sodium hydroxide solution for oxidative polymerization to generate a polydopamine solution; uniformly mixing the two components according to a certain ratio, and then adding a cross-linking agent ethylene glycol glycidyl ether; and placing the mixture in a 37 DEG C constant-temperature box for 12 hours to form gel. Herein, the glucan used in the invention is a high-water-solubilitynatural polysaccharide secreted by bacteria and has good biocompatibility and degradability, therefore, a large number of micropore structures are generated after gel is formed, the large specific surface area can provide a large number of loading sites for polydopamine, cells can enter the gel easily and adhere to the polydopamine, and the porous hydrogel scaffold material is excellent and has wide application prospects in wound repair and biological tissue engineering.

Description

technical field [0001] The invention relates to the field of biological scaffolds, in particular to a polydopamine-doped dextran hydrogel porous scaffold, a preparation method and application thereof. Background technique [0002] Dextran is a microbial polysaccharide composed of glucose as a monosaccharide secreted by some microorganisms during the growth process, in which the glucose units are connected by glycosidic bonds. Dextran has good biocompatibility and degradability, but the adhesion of dextran gel is weak, and it is easy to fall off from the body in practical applications, thus hindering its further application. [0003] Studies have found that the super-adhesive protein secreted by marine mussels through their foot glands can firmly adhere to the surface of various materials in humid environments such as seawater. Polydopamine is a bionic material for mussels. Mussel Adhesin has a similar structure and superadhesive properties. [0004] The application of hydr...

Claims

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Application Information

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IPC IPC(8): C08J9/00C08J3/075A61L26/00C12N5/00C12N5/071C08L5/02C08L79/04
CPCC08J9/0061C08J3/075A61L26/008A61L26/0085A61L26/0066A61L26/0052C12N5/0062C12N5/0656A61L2300/412C08J2305/02C08J2479/04C08J2379/04C08J2405/02C12N2513/00C12N2533/30C12N2533/70C08L5/02C08L79/04
Inventor 齐晓亮
Owner WENZHOU MEDICAL UNIV
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