Chemical component-limited high-efficiency fed-batch culture medium, and preparation method and application thereof
A feed medium and chemical composition technology, applied in the field of feed medium, can solve the problems of cell density and antibody protein expression that need to be further improved, difficulty in preparing high-concentration products, and precipitation of nutrients, so as to reduce the dilution effect, Low production cost and the effect of avoiding precipitation
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[0058] Select the optimal formula composition and content of the present invention to prepare, wherein the composition and formula of Duo Ning feeding medium A:
[0059]
[0060]
[0061] The method of preparing Donin feed medium A is as follows:
[0062] (1) Quantitatively weigh each component in the feed medium according to the prepared volume, and mix well;
[0063] (2) dissolving the feed medium mixture in step (1) in ultrapure water, controlling the water temperature at 20-30° C., and adding sodium hydroxide to adjust the pH to promote dissolution;
[0064] (3) Add 75g of glucose in every liter of culture medium and stir evenly;
[0065] (4) adjust the pH between 6.7-6.9, and use ultrapure water to make it volume;
[0066] (5) Filter with a 0.22 μm filter membrane and store at 2-8°C.
[0067] Composition and formula of Donin feed medium B:
[0068] amino acid Contentmg / L amino acid Contentmg / L L-tryptophan 28000 L-cysteine 24400 L-tyr...
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