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Micro-fluidic chip, preparation method thereof and cell sorting method

A microfluidic chip and cell technology, applied in the field of microfluidics, can solve the problem of unsatisfactory screening and separation of single cells, and achieve the effect of improving screening and separation efficiency, good controllability, and low cost

Active Publication Date: 2020-10-13
SHENZHEN TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide a microfluidic chip and its preparation method and cell sorting method, aiming to solve the problem of unsatisfactory single-cell screening and separation in the existing droplet microfluidic technology. question

Method used

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  • Micro-fluidic chip, preparation method thereof and cell sorting method
  • Micro-fluidic chip, preparation method thereof and cell sorting method
  • Micro-fluidic chip, preparation method thereof and cell sorting method

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Embodiment 1

[0073] Step 1: Take a certain amount of PDMS prepolymer, put it into a cuboid container, and then solidify it to form a PDMS with a length, width, and height of 50mm, 27mm, and 7mm, respectively.

[0074] Step 2: Set the laser direct writing program for the specific position of the pipeline in the PDMS substrate, and use the laser direct writing technology to engrave the inside of the PDMS substrate such as figure 1 and image 3 The shown droplet generation pipeline, screening and separation pipeline, after the processing is completed, the air pump is used as the driving force to remove the processing residue in the micropipe. Among them, the parameters of the laser direct writing technology are: duration 100fs, wavelength 800nm, frequency 1000Hz ; Each liquid phase is passed into ports A, B, and C respectively,

[0075] Step Four: For image 3 As shown in the screening and separation pipeline structure, evenly apply ultraviolet curing UV glue on the back of the PDMS where t...

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Abstract

The invention discloses a micro-fluidic chip, a preparation method thereof and a cell sorting method. The preparation method of the micro-fluidic chip comprises the following steps of: providing a polydimethylsiloxane substrate; engraving a pipeline in the polydimethylsiloxane substrate by using a laser direct writing technology; a plurality of electrodes distributed in an array are arranged on the bottom surface of the polydimethylsiloxane substrate, and a photoelectric sensor is arranged on one side of a main pipeline, so that the micro-fluidic chip is obtained. Compared with the prior art,the preparation method has the advantages of simple preparation, lower cost, large-scale production, and good controllability.

Description

technical field [0001] The invention relates to the field of microfluidics, in particular to a microfluidic chip, a preparation method thereof, and a cell sorting method. Background technique [0002] Droplet microfluidic technology has attracted extensive attention since the 21st century because of its advantages such as easy fluid manipulation, high monodispersity, miniaturization, low cost, high sensitivity, and high throughput. At present, the application of microfluidic droplet technology is mainly used for the manipulation of droplets, such as the realization of splitting, fusion, mixing, sorting, fusion and other functions of droplets. The sorting of droplets has been widely used in research fields such as biology, chemistry and medicine. [0003] At present, in the differential analysis experiments for single cells, single cell manipulation is the key issue. However, there are still some problems in the sorting technology of droplets. For example, the electric fie...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M1/34C12M1/00C12N5/078
CPCB01L3/502707B01L2400/0415C12M47/04C12N5/0634C12N5/0641C12N2509/00
Inventor 王萌江阳光
Owner SHENZHEN TECH UNIV