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Method for improving quality of unfrozen pig sperms

A pig sperm and quality technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of unclear internal damage mechanism and achieve the effects of improving quality, improving centrifugation effect, and improving vitality

Active Publication Date: 2020-10-16
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In recent years, the existing technology has conducted detailed research on the external conditions that affect the quality of cryopreserved sperm, and the external conditions can be controlled to an appropriate range during the cryopreservation process to minimize the impact of external conditions; however, the internal damage mechanism However, it is not clear how to avoid the quality loss of pig spermatozoa during the freezing process to the greatest extent has become a technical problem to be solved

Method used

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  • Method for improving quality of unfrozen pig sperms

Examples

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Effect test

Embodiment 1

[0031] A method for improving the quality of pig sperm after thawing, comprising the following steps:

[0032] (1) After the collected qualified semen is diluted with semen protection diluent within 2 hours, it is placed in a 25°C incubator for 1 hour, and the semen is mixed from time to time, and then placed in a 17°C incubator for static equilibrium treatment 2 hours, mixing at irregular times during the period, and the constant temperature equilibrium treatment is completed; the qualified semen is selected by the following method: select the semen with a slight fishy smell and milky white for microscopic examination, and the semen with an activity of more than 90% and a density between 200 and 400 million / mL For qualified semen, the semen protection diluent is made of the following components by weight: 1.25 parts of ethylenediaminetetraacetic acid, 1.25 parts of sodium citrate dihydrate, 0.75 parts of potassium chloride, 1.25 parts of sodium bicarbonate, 36.50 parts of gluc...

Embodiment 2

[0037] A method for improving the quality of pig sperm after thawing, comprising the following steps:

[0038] (1) After the collected qualified semen is diluted with semen protection diluent within 2 hours, it is placed in a 25°C incubator for 1 hour, during which the semen is mixed from time to time, and then placed in a 17°C incubator for static equilibrium treatment 2 hours, mixing at irregular times during the period, and the constant temperature equilibrium treatment is completed; the qualified semen is selected by the following method: select the semen with a slight fishy smell and milky white for microscopic examination, and the semen with an activity of more than 90% and a density between 200 and 400 million / mL For qualified semen, the semen protection diluent is made of the following components by weight: 1.20 parts of ethylenediaminetetraacetic acid, 1.20 parts of sodium citrate dihydrate, 0.70 parts of potassium chloride, 1.20 parts of sodium bicarbonate, 36.00 part...

Embodiment 3

[0043] A method for improving the quality of pig sperm after thawing, comprising the following steps:

[0044] (1) After the collected qualified semen is diluted with semen protection diluent within 2 hours, it is placed in a 25°C incubator for 1 hour, during which the semen is mixed from time to time, and then placed in a 17°C incubator for static equilibrium treatment 2 hours, mixing at irregular times during the period, and the constant temperature equilibrium treatment is completed; the qualified semen is selected by the following method: select the semen with a slight fishy smell and milky white for microscopic examination, and the semen with an activity of more than 90% and a density between 200 and 400 million / mL For qualified semen, the semen protection diluent is made of the following components by weight: 1.30 parts of ethylenediaminetetraacetic acid, 1.30 parts of sodium citrate dihydrate, 0.80 parts of potassium chloride, 1.30 parts of sodium bicarbonate, 37.00 part...

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Abstract

The invention discloses a method for improving the quality of unfrozen pig sperms, which comprises the following steps: (1) diluting collected qualified sperms by using a sperm protection diluent, andcarrying out constant-temperature balance treatment; (2) centrifuging the sperm, adding a frozen basic solution I into the obtained centrifugal precipitate, resuspending, and carrying out constant-temperature balancing treatment in a constant-temperature box; (3) adding the frozen basic liquid II into the container, uniformly mixing, filling into a thin tube, sealing, fumigating on liquid nitrogen or freezing by using a program freezer, and storing in the liquid nitrogen; and (4) when the sperm is unfrozen, taking out the tubule from the liquid nitrogen, incubating in a water bath kettle, andthen adding an unfreezing diluent to complete the unfreezing. According to the invention, caffeic acid phenethyl ester and hydroxyethyl starch with corresponding concentrations are jointly added intothe freezing base solution I, and the caffeic acid phenethyl ester and hydroxyethyl starch generate a synergistic effect, so that the vitality, plasma membrane integrity rate, acrosome integrity rateand DNA integrity rate of the unfrozen pig sperms can be effectively improved, and the quality of the frozen pig sperms is improved.

Description

technical field [0001] The invention relates to the technical field of cryopreservation of pig sperm, in particular to a method for improving the quality of pig sperm after thawing. Background technique [0002] With the rapid rise of pig artificial insemination technology, most pig farmers have adopted this method to breed piglets in order to reduce the cost of boar breeding. The cryopreservation of semen can make the use of semen not limited by time, place and object of use, and significantly increase the use time and utilization rate of the semen of good boars. These advantages make artificial insemination technology more popular. Therefore, frozen semen technology plays an important role. [0003] The cryopreservation of semen can effectively preserve the genetic breeding and germplasm resources of organisms. With the rapid development of pig frozen semen production technology and artificial insemination technology, the conception rate and number of piglets of breeding...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 李兆华张志彬钱芙蓉菅芯蕊王珺于永生昭日格图王俊恒李彦春刘向光张鑫金庆国柳海星高见红孟庆祝丁德李闯吴德忠李淑梅张志民张健
Owner JILIN ACAD OF AGRI SCI
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