A high-efficiency microbial remediation method for cadmium-contaminated sediments
A technology of high-efficiency microorganisms and remediation methods, which is applied in the field of nanomaterials combined with microorganisms, can solve the problems such as the inability to meet the growth and work of SRB, and achieve the effects of enhancing the immobilization effect, reducing the risk of toxicity, and rapidly reducing the cadmium content.
Active Publication Date: 2022-02-25
BEIHANG UNIV
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Problems solved by technology
However, there are many difficulties in the practical application of SRB's biological immobilization technology. For example, the invention patent with the publication number CN107138521A and the title of the invention is "a microbial remediation method for cadmium-contaminated sediment", which was added to the sediment system by SRB. After that, it takes up to 166 days to achieve a certain repair effect
This is mainly because some environmental conditions, such as pH, oxidation-reduction potential, electron donor, etc., cannot meet the growth and work of SRB, and it takes a long time for SRB to adapt to the environment of the research system.
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[0026] In order to facilitate the understanding of the present invention, the present invention will be described in more detail below in conjunction with specific examples and comparative examples.
[0027] An efficient microbial remediation method for cadmium-contaminated deposits, comprising the following steps:
[0028] Step 1, taking natural reservoir sediment for SRB enrichment, domestication and expanded cultivation, to obtain purified SRB bacterial liquid;
[0029] Specifically, (1.1) prepare PostgateB medium and improved PostgateB medium, wherein, the PostgateB medium formula is as follows: KH2PO4 0.5g / L, NH4Cl 1g / L, CaSO4 1g / L, MgSO4 7H2O 2g / L, sodium lactate 2g / L, yeast extract 1g / L, ascorbic acid 0.1g / L, mercaptoacetic acid 0.1g / L, FeSO4 7H2O 0.5g / L; the formula of the improved PostgateB medium is as follows: KH2PO4 0.5g / L, NH4Cl 1g / L L, CaSO4 1g / L, MgSO4·7H2O2g / L, sodium lactate 2g / L, yeast extract 1g / L, ascorbic acid 0.1g / L, thioglycolic acid 0.1g / L, Na2SO4 0.25...
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A high-efficiency microbial remediation method for cadmium-contaminated sediments, comprising the following steps: Step 1, taking natural reservoir sediment for SRB enrichment, domestication and expanded culture to obtain purified SRB bacterial liquid; Step 2, the expanded cultured Purified SRB bacteria solution and nano-zero-valent iron jointly repaired Cd-contaminated deposits; step 3, collecting Cd-contaminated deposits repaired by using purified SRB bacteria solution and nano-zero-valent iron at regular intervals; The dominant species of typical SRB at the family level included Desulfolobus, Desulfoenterobacteriaceae, Desulfobacteriaceae, Sulfur-Reducing Bacteriaceae and Desulfovibrioceae. This method is easy to achieve in-situ repair, and has the advantages of simple operation, good repair effect, and environmental friendliness. The immobilization effect of Cd can be enhanced, and the cadmium content in the overlying water can be rapidly reduced, and when there is continuous new cadmium pollution in the overlying water environment of the cadmium-polluted sediment, the method of the invention can realize continuous and efficient restoration.
Description
technical field [0001] The invention relates to the technical field of remediation of heavy metal pollutants, in particular to a method for remediating cadmium-contaminated deposits by combining nanomaterials with microorganisms. Background technique [0002] Due to the characteristics of high toxicity, non-degradability and enrichment along the food chain of heavy metals, heavy metal pollution in water is a serious hazard to the ecological environment and human health. However, heavy metals in the water environment eventually accumulate in sediments under the joint action of adsorption, precipitation, co-precipitation, and biological activities, resulting in more severe heavy metal pollution in sediments. In addition, sediments play a key role in the transportation, morphological transformation and food chain transfer of heavy metals. Once the sediments are disturbed by natural or human activities, the heavy metals accumulated in the sediments will be released into the aqu...
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IPC IPC(8): B09C1/00C12N1/36C12R1/01C02F101/20
CPCC02F3/34C12N1/36C12N1/20C12N1/02C02F2101/20
Inventor 范文宏赵晴萧晟韬彭位华李晓敏
Owner BEIHANG UNIV