Fusion protein and use thereof

Abstract

The invention relates to a fusion protein and use thereof. The fusion protein comprises, from N to C terminals, a first moiety, an Fc segment, a linker moiety comprising a moiety selected from the group consisting of a linker and a protein or polypeptide selected from the group consisting of IL2 or scFv, and a substrate moiety of transpeptidase A; the linker comprises a sequence selected from thegroup consisting of (1) (GGGGS) n, wherein when the linker moiety comprises the protein or polypeptide and linker, n>=1; when the linker moiety only comprises the joint, n>=3; and (2) (EAAK) n, n >= 1; the substrate moiety comprises a sequence as shown in LPXTG. The fusion protein can be directly connected to cells to enable the cells to have targeting property, is simpler than an existing methodfor preparing targeting cells through cell transfection, and can also reduce the risk possibly generated by effector cell genome operation at the same time.

Description

technical field [0001] The present invention relates to the field of proteins, in particular fusion proteins suitable for linking antibody Fc regions to cells. Background technique [0002] Cell therapy is a new technology for disease treatment that has emerged in recent years. It refers to the use of the characteristics of certain cells with specific functions (such as stem cells and immune cells), and the cells with enhanced functions produced after specific treatment, and then infused into the body to treat diseases. the goal of. With the continuous development of stem cell therapy, immune cell therapy and gene editing and other basic theories, technical means and clinical medical exploration research, cell therapy products provide new treatment ideas and methods for some serious and refractory diseases, showing more and more The higher the application value. [0003] Targeted therapy is a drug therapy that treats diseases by interfering with specific molecules. Targete...

AI Technical Summary

Problems solved by technology

Although it has been found that transpeptidase A can directly link various molecules (such as biotin, polypeptides, antibody heavy chain variable region VHH fragments) to the cell surface (US20160122707A1; Jeong HJ, et al., Generation of Ca2+-independent sortase A Mutants with enhanced activity for protein and cell surface labeling. PLoS One. 2017Dec 4; 12(12):e0189068; Chen I, et al., A general strategy for the evolution of bond-forming enzymes using yeast display. Proc Natl Acad Sci U S A .2011Jul 12; 108(28):11399-404.), however, due to the large molecular weight and complex structure of the Fc segment, there is no research report that can successfully pass the transpeptidase A to contain the Fc segment (fragment crystallizable region ) whole antibody or Fc fusion protein directly attached to the cell surface

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