Infrared ii region fluorescent gold nanoclusters and its preparation and application
A technology of fluorescent gold nanometers and gold nanoclusters, which is applied in the field of fluorescent materials in the infrared II region, can solve the problems of non-compliance, cytotoxic side effects, the preparation and imaging application of gold nanomaterials without research, and achieves improved biocompatibility. sexual effect
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Embodiment 1
[0061] (1) The ligand-template molecule RNase-A was dissolved in water to prepare a ligand-template solution with a concentration of 10 mg / mL. Further, tetrachloroauric acid was dissolved in water to obtain a chloroauric acid solution having a concentration of 15 mM. Both solutions were prepared in 500 μL.
[0062] (2) Add 50 μL of 1M sodium hydroxide aqueous solution dropwise into the chloroauric acid solution, and observe that the golden yellow chloroauric acid solution fades, and then add the ligand template solution to it to form a mixed solution. The pH of the mixed solution is about 11.
[0063] (3) After mixing thoroughly for 5 minutes, add 10 μL of reducing agent NaBH 4 An aqueous solution (concentration 15 mM) was added dropwise to the above mixed solution. The mixed solution was placed in a refrigerator at 4°C overnight, and a dark brown transparent and clear mixed solution was obtained, indicating that the reaction was complete, and the solution contained a large...
Embodiment 2
[0066] Prepare gold nanoclusters according to the method of Example 1, the difference is that RNase-A is replaced by equimolar amounts of β-lactoglobulin, bovine blood serum albumin (BSA), dihydrolipoic acid, dihydrosulfur-sulfur betaine, glutathione, mercaptoethanol or bioengineering custom polypeptide (its amino acid sequence structure is shown in Table 1).
[0067] Test the fluorescence properties of the infrared II region of several gold nanoclusters prepared in the above examples, the structures are as shown in Table 1, and N in the table 巯基 / N 共轭 Represents the molar ratio of the total number of sulfhydryl groups in the ligand molecule to the total number of conjugated molecules. It can be seen from the table that the eight kinds of ligand molecules can emit fluorescence in the infrared region II, and ribonuclease, β-lactoglobulin, dihydrosulfur-sulfobetaine, and bioengineered polypeptides (containing sulfhydryl and The fluorescence emission peaks of gold nanoclusters pr...
Embodiment 3
[0077] Prepare gold nanomaterials according to the method of Example 1, the difference is that RNase-A is replaced by small molecule amino acids: cysteine, histidine and tyrosine, and the molar ratio of the three amino acids is changed to prepare different gold nanomaterials.
[0078] Table 2 shows the effects of different amino acid ratios on the emission peaks and morphology of gold nanomaterials. In Table 2, NA represents no fluorescence emission. It can be seen that only a specific ratio of amino acids can make the emission peak of the gold nanoclusters red-shift to the infrared II region.
[0079] Table 2 Effects of different ratios of amino acids on the emission peaks and morphology of gold nanoclusters
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