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Nano-material immobilized microorganism remediation agent and preparation method and application thereof

A technology for immobilizing microorganisms and nanomaterials, which is applied in the field of nanomaterial immobilized microbial repair agents and its preparation, and can solve the problems of low repair efficiency by adding free microorganisms and low mechanical strength of immobilized microbial agents

Active Publication Date: 2020-10-30
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Technical problem to be solved: the present invention provides a nanomaterial immobilized microbial remediation agent and its preparation method and application, which can overcome the problems of low repair efficiency of adding free microorganisms in actual polluted soil, low mechanical strength of the original immobilized microbial agent, etc. , helps to promote the growth and reproduction of degrading microorganisms, improves the mechanical strength and mass transfer efficiency of immobilized microbial agents, and realizes the efficient, green and economical sustainable restoration of PAHs-contaminated soil. Good potential for development and application

Method used

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  • Nano-material immobilized microorganism remediation agent and preparation method and application thereof
  • Nano-material immobilized microorganism remediation agent and preparation method and application thereof
  • Nano-material immobilized microorganism remediation agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Graphene oxide-based nanocomposite immobilized HPD-2 repair agent on the removal effect of PAHs in soil

[0018] Prepare LB medium, the ingredients and dosage: yeast extract 5.0g / L, peptone 10.0g / L, NaCl 10.0g / L, the rest is deionized water, adjust the pH to 7.0, sterilize at 0.12MPa, 121℃ Bacteria 20min standby. Take out the strain of Paracoccus ammophilus stored at -80°C, draw 100 μL of bacterial liquid and inoculate it into 10 mL of liquid LB medium for rejuvenation culture, cultivate it at 30°C and 150 r / min for 8 hours, and then inoculate it at a volume ratio of 10%. The culture was continued for 16 h in liquid LB medium. After centrifuging at a speed of 6000r / min for 5min, the logarithmic growth phase cells of the above bacteria were obtained; washed twice with phosphate buffer and resuspended to adjust the OD of the bacterial suspension 600 value to 1.0, centrifuge and concentrate 100mL of the bacterial suspension to obtain Paracoccus ammonophilus ba...

Embodiment 2

[0022] Example 2: Graphene oxide-based nanocomposite immobilized HPD-2 repair agent on the removal effect of PAHs in soil

[0023] Compared with Example 1, LB medium was added to the sodium alginate solution in a volume ratio of 5% in this example, and other steps and parameters were the same as in Example 1, and the graphene-based nanocomposite immobilized HPD was prepared. -2 Repair Potion. The soil collected from a contaminated site of a coking plant in Nanjing was used as the test soil. The content of PAHs in the soil was 344.48 mg / kg. The graphene-based nanocomposite immobilized HPD-2 restoration agent prepared above was added at a dosage of 3wt.%. Add to the soil. After 35 days of remediation, the removal rate of PAHs in the soil immobilized with HPD-2 remediation agent using graphene oxide-based nanocomposites was 68.24%, which was not significantly different from that of the immobilized remediation agent added with 10% LB medium. Immobilized HPD-2 remediation agent t...

Embodiment 3

[0024]Example 3: Graphene oxide-based nanocomposite immobilized HPD-2 repair agent on the removal effect of PAHs in soil

[0025] Compared with Examples 1 and 2, LB medium was added to the sodium alginate solution in a volume ratio of 8% in this example, and other steps and parameters were the same as in Examples 1 and 2, and the graphene-based nanocomposite was prepared. Material-immobilized HPD-2 restoration agent. The soil collected from a contaminated site of a coking plant in Nanjing was used as the test soil. The content of PAHs in the soil was 344.48 mg / kg. The graphene-based nanocomposite immobilized HPD-2 restoration agent prepared above was added at a dosage of 3wt.%. Add to the soil. After 35 days of remediation, the removal rate of PAHs in the soil immobilized with graphene oxide-based nanocomposite HPD-2 remediation agent was 71.35%, which was not significantly different from that of the immobilized remediation agent added with 10% LB medium, and compared with th...

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Abstract

The invention discloses a nano-material immobilized microorganism remediation agent and a preparation method and application thereof. The preparation method comprises the following steps of: (1) preparation of paracoccus aminovorans thalli; (2) preparation of an embedding agent graphene oxide-based nanocomposite material; (3) preparation of a cross-linking agent CaCl2 solution; and (4) preparationof the nano-material immobilized microorganism remediation agent. The graphene oxide-based nano-material immobilized microorganism remediation agent prepared by the preparation method disclosed by the invention is high in applicability to the environment, stable exertion of a degradation function of polycyclic aromatic hydrocarbon degradation microorganisms is guaranteed, the degradation efficiency of a traditional immobilized microorganism preparation is improved, and the microbial remediation period of polycyclic aromatic hydrocarbon contaminated soil is shortened; the preparation method issimple to operate, low in cost and good in effect, has a prospect of large-scale industrial production, is suitable for in-situ remediation of polycyclic aromatic hydrocarbon contaminated soil, and has no secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of contaminated soil, and in particular relates to a nanomaterial-immobilized microbial remediation agent and a preparation method and application thereof. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of organic pollutants widely present in the soil environment, mainly derived from incomplete combustion of fossil fuels and biomass. In recent years, PAHs pollution in my country's soil has become increasingly serious. In 2014, the "National Soil Pollution Survey Bulletin" jointly issued by the Ministry of Environmental Protection and the Ministry of Land and Resources showed that the rate of PAHs-contaminated soil was 1.4%. Combination of components and other characteristics seriously endanger the quality and safety of agricultural products and the safety of human settlements. Therefore, how to effectively control a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/14B09C1/10C09K17/40C09K17/14C12R1/01
CPCC12N11/10C12N11/14B09C1/10C09K17/40C09K17/14
Inventor 任文杰滕应毛婷玉骆永明
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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