Terahertz spectroscopy system, vitamin D detection method, sample and sample preparation method

Abstract

The invention discloses a terahertz spectrum system, a vitamin D detection method, a sample and a sample preparation method, and solves the problem that the existing method for detecting vitamin D is destructive detection, which damages the sample and requires a new nondestructive detection method. Problem, the present invention includes the detection of vitamin D by using a terahertz time-domain spectroscopy system, the structure of the vitamin D is a sandwich structure of high-density polyethylene top layer-vitamin D layer-high-density polyethylene bottom layer, and the sandwich structure of vitamin D adopts Carried out by tableting. The invention has the advantages of non-destructive detection of vitamin D, rapid and accurate detection, and the like.

Description

technical field [0001] The invention relates to the technical field of terahertz spectrum measurement, in particular to a terahertz time-domain spectrum system and vitamin D. Background technique [0002] Vitamin D (referred to as VD) is a fat-soluble vitamin, which is an essential nutrient for maintaining the life of higher animals. It is a general term for a group of sterol derivatives with anti-rickets effects and similar structures. There are many kinds of known vitamin D, the most important ones are vitamin D3 (cholecalciferol, cholecalciferol) and vitamin D2 (calciferol). Vitamin D in the diet mainly comes from animal foods such as cod liver, egg yolk, butter, etc. After ingestion, it is absorbed in the small intestine in the presence of bile and transported into the blood in the form of chylomicrons. Transformed into 1,25-dihydroxyvitamin D3, which has biological activity and can stimulate the synthesis of intestinal mucosal calcium-binding protein (CaBP), promote ca...

AI Technical Summary

Problems solved by technology

At present, most countries use chromatography as the standard method for vitamin detection. However, the equipment of chromatography technology is expensive, and the steps are cumbersome, the operation time is long, and it is easy to lose. The technical requirements for operators and laboratory equipment conditions are very high.

Moreover, vitamin D has a high degree of structural specificity, and its structure changes slightly. If the total treatment time is too long, its activity will change greatly. Therefore, many traditional detection methods may lead to the loss of its function and loss of activity at the same time. Content of vulnerable vitamins

High-performance liquid chromatography tandem mass spectrometry mainly uses isotope internal standard detection. This method has high sensitivity, but isotope standard is expensive and is not suitable for the detection of a large number of samples in daily work. The price of purchasing isotope internal standard is much higher than the detection cost of general laboratories. times, and there is no domestic manufacturer, it needs to be purchased from abroad, and the waiting period is very long

At present, there are still some new methods, such as electrochemistry, colorimetry, etc., which can be applied to the detection of vitamin D, but these methods are insufficient, and they are all destructive detection methods, which will damage the sample to a certain extent.

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