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Surface plasmon resonance sensing chip, preparation method thereof and sensing equipment

A sensor chip and resonance membrane technology, which is applied in the field of ion resonance sensor chip and its preparation, can solve the problems of sensitivity, selectivity, and the cost cannot meet the requirements, and achieves good chip reproducibility, simple operation steps, and structure. clear effect

Active Publication Date: 2020-10-30
BEIJING INST OF CLOTHING TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, in the process of realizing the present invention, the applicant found that the G+ bacteria detection technology in the prior art cannot meet the needs in terms of sensitivity, selectivity, cost, etc., and it is particularly necessary to provide a sensor chip for detecting G+ bacteria

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  • Surface plasmon resonance sensing chip, preparation method thereof and sensing equipment
  • Surface plasmon resonance sensing chip, preparation method thereof and sensing equipment
  • Surface plasmon resonance sensing chip, preparation method thereof and sensing equipment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: SPR sensor chip for the detection of G+ bacteria

[0035] This embodiment provides an SPR sensor chip for the detection of G+ bacteria.

[0036] figure 1 It is a schematic structural diagram of an SPR sensor chip according to Embodiment 1 of the present invention. Such as figure 1 As shown, the SPR sensor chip of this embodiment includes: an optical waveguide coupling plate 10; a resonant film 20 formed on the optical waveguide coupling plate; a sensitive film 30 formed on the resonant film, which includes a probe coupled on the resonant film molecular.

[0037] Each component of the SPR sensor chip of this embodiment will be described in detail below.

[0038] The optical waveguide coupling sheet 10 is a transparent substrate, such as a glass substrate, a sapphire substrate, a silicon dioxide substrate, a prism, and the like.

[0039] The resonant film 20 is a thin film formed of a material capable of causing surface plasmon resonance, such as a meta...

Embodiment 2

[0049] Embodiment 2: SPR sensor chip and its preparation method, SPR sensor equipment

[0050] This embodiment provides an SPR sensor chip for the detection of G+ bacteria. Please refer to figure 1 , the present embodiment SPR sensing chip comprises: a glass substrate; a gold film with a thickness of 10nm evaporated on the glass substrate; a sensitive film formed on the gold film, which includes the S1-1 molecular chain Potassium penicillin G molecules coupled to the surface of a gold film.

[0051] This embodiment also provides the preparation method of the above SPR sensor chip. figure 2 It is a flowchart of the preparation method of the SPR sensor chip in the second embodiment of the present invention. The preparation method includes:

[0052] Step S21: using vacuum evaporation technology, evaporating a gold film with a thickness of 10 nm on the surface of the glass substrate;

[0053] During the coating process, set the vacuum degree of the thermal evaporation coati...

Embodiment 3

[0074] Embodiment 3: SPR sensor chip and its preparation method

[0075] This embodiment provides an SPR sensor chip for the detection of G+ bacteria and its preparation method. The differences between it and the second embodiment are: ① different coupling molecular chains are used; ② the preparation method of the resonance membrane is different.

[0076] In the present embodiment, the SPR sensor chip comprises: a glass substrate; a gold film sputtered on the glass substrate with a thickness of 50nm; a sensitive film formed on the gold film, which includes the S1-2 molecular chain Potassium penicillin G molecules coupled to the surface of a gold film.

[0077] In the present embodiment, the preparation method of the above SPR sensor chip comprises:

[0078] Step S31: Coating a gold film with a thickness of 50 nm on the surface of the glass substrate by magnetron sputtering technology;

[0079] Step S32: Soak the glass substrate obtained in step S31 in 100mmol / L of compound ...

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Abstract

The invention provides a surface plasma resonance sensing chip, a preparation method thereof and sensing equipment. The SPR sensing chip comprises an optical waveguide coupling sheet; a resonance filmformed on the optical waveguide coupling sheet; and a sensitive film formed on the resonant film, the sensitive film including probe molecules coupled to the resonant film. Wherein the probe moleculeis selected from one or more of the following groups: 1, a probe molecule, 2, a probe molecule and 3, a probe molecule, penicillin G, benicillin, methoxybenzylpenicillin sodium, the composition comprises chloro-o-penicillin sodium, ampicillin, ampicillin, oxyampicillin, sodium ethoxynaphthalene penicillin, carbenicillin, proventricillin I, proventricillin II, proventricillin IV, proventricillin V, proventricillin hydroxyampicillin, cefotaxime sodium, erythromycin, lincomycin, northern mycin, tylosin phosphate, chlorlincomycin, spiramycin, bacitracin or neomycin. Compared with the traditionalG + bacteria detection technology, the sensing chip has the advantages of rapidness, simplicity, convenience, quantification, ultra-sensitivity, low cost and the like.

Description

technical field [0001] The present invention relates to the field of biological chips, in particular to a surface plasmon resonance (Surface Plasmon Resonance, referred to as SPR) sensor chip for Gram-positive bacteria detection and a preparation method thereof; for fungi, Gram-positive bacteria ( A multi-species and multi-channel SPR sensor chip for high-precision detection of G+ bacteria) and Gram-negative bacteria (G- bacteria) and a preparation method thereof; and a sensing device using the above two SPR sensor chips. Background technique [0002] Bacteria refers to one of the groups of microorganisms, and it is also the most abundant group of all living things. Common strains that affect human health include fungi, Gram+ bacteria and Gram-bacteria. Rapidly distinguishing the types of pathogenic bacteria is very important in clinical testing, and can provide effective medication guidance for disease treatment. [0003] SPR technology is a new technology that applies th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/552G01N21/03G01N33/569
CPCG01N21/554G01N21/031G01N33/56911G01N33/56961G01N2021/0328G01N2021/0346Y02A50/30
Inventor 张洪艳李叶张秀芹刘亚侠
Owner BEIJING INST OF CLOTHING TECH