Method for strengthening rhodococcus to degrade aniline by utilizing environmental stress
A technology for the degradation of Rhodococcus and bacteria, which is applied in the field of biological enhancement treatment of environmental pollution, can solve the problems such as the inability to overcome the weakening of aniline treatment capacity, and achieve the effects of improving growth and aniline degradation, no secondary pollution, and simple and convenient procedures
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Embodiment 1
[0035] Embodiment 1 Utilizes phenol stress to strengthen the method for improving the degradability of Rhodococcus sp.
[0036] 1. Culture medium formula
[0037] The formula of LB medium is: peptone 10g, yeast powder 5g, NaCl 10g, agar 18-20g, pH 7.0, replenish water to 1000mL; sterilize under high temperature and high pressure at 121℃ for 20min;
[0038] The formula of inorganic salt solid medium is: Na 2 HPO 4 12H 2 O 2g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, 5mL trace element solution, 18-20g agar, pH 7.0, replenish water to 1000mL; sterilize at 121°C for 20min with damp heat, high temperature and high pressure;
[0039] The formula of inorganic salt liquid medium is: Na 2 HPO 4 12H 2 O 2g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, 5mL trace element solution, pH 7.0, add water to 1000mL; sterilize at 121℃ for 20min with damp heat, high temperature and high pressure;
[0040] Trace element solution formula: EDTA0.5g / L, ZnSO 4 ·7H 2 O 0.22g / L, CaCl 2 0.055g / L,...
Embodiment 2
[0047] Embodiment 2 utilizes NaCl stress to strengthen the method for the degradability of Rhodococcus sp.
[0048] 1. Culture medium formula
[0049] With embodiment 1.
[0050] 2. Method
[0051] 1. Inoculate the preserved Rhodococcus sp.AN-P1 on slant LB medium and culture it at 30°C for 36-48 hours to obtain the T1 generation strain of Rhodococcus sp.AN-P1; then transfer the T1 generation strain to The slant inorganic salt solid medium containing 1000mg / L aniline was also cultured at 30°C for 36-48 hours to obtain the activated Rhodococcus sp.AN-P1 strain;
[0052] 2. Inoculate the activated Rhodococcus sp.AN-P1 in the inorganic salt liquid medium containing 1000 mg / L aniline, and culture it under aerobic conditions at 30°C for 18-24 hours to obtain Rhodococcus sp.AN-P1 which can efficiently degrade aniline. P1 seed solution;
[0053] 3. Apply 20-45g / L NaCl stress to the seed solution mentioned in (2) respectively, and aerobically culture it under the stress condition ...
Embodiment 3
[0056] Embodiment 3 utilizes low temperature stress to strengthen the method for the degradability of Rhodococcus sp.
[0057] 1. Culture medium formula
[0058] With embodiment 1.
[0059] 2. Method
[0060] 1. Inoculate the preserved Rhodococcus sp.AN-P1 on slant LB medium and culture it at 30°C for 36-48 hours to obtain the T1 generation strain of Rhodococcus sp.AN-P1; then transfer the T1 generation strain to The slant inorganic salt solid medium containing 1000mg / L aniline was also cultured at 30°C for 36-48 hours to obtain the activated Rhodococcus sp.AN-P1 strain;
[0061] 2. Inoculate the activated Rhodococcus sp.AN-P1 in the inorganic salt liquid medium containing 1000 mg / L aniline, and culture it under aerobic conditions at 30°C for 18-24 hours to obtain Rhodococcus sp.AN-P1 which can efficiently degrade aniline. P1 seed solution;
[0062] 3. Apply low temperature stress at 15-25°C to the seed liquids described in (2), and culture them aerobically for 18-30 hours...
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