Preparation method and application of a lignin-based antitumor drug carrier
An anti-tumor drug, a lignin-based technology is applied in the field of preparation of a lignin-based anti-tumor drug carrier, and can solve the problems of lack of pH response characteristics, low drug loading and encapsulation rate, inability to target drug release, and the like, Achieve sensitive pH response performance, significant economic and social benefits, and reduce toxic and side effects
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Embodiment 1
[0027] A preparation method of a lignin-based antitumor drug carrier of the present invention comprises the following steps:
[0028] (1) Preparation of carrier components: The carrier is composed of the following weights: 100 parts of alkali lignin, 35 parts of formaldehyde, 60 parts of sulfonating reagent, 60 parts of cationizing reagent, 40 parts of cationic surfactant, organic solvent 550 parts and 25 parts of antineoplastic drugs are formulated;
[0029] (2) Add water to alkali lignin to make a solution, adjust the pH to 11, heat up to 70°C, add formaldehyde, react for 3 hours to obtain hydroxymethylated lignin, then add sulfonating reagent, react for 2.5 hours to obtain sulfonamide Then add cationic reagents to react for 2 hours, transfer the reacted solution to a dialysis bag with a molecular weight cut-off of 2000 for dialysis and purification for 6 days (days), spin evaporate, and freeze-dry to obtain amphoteric lignin;
[0030] (3) Dissolve the amphoteric lignin in ...
Embodiment 2
[0033] A preparation method of a lignin-based antitumor drug carrier of the present invention comprises the following steps:
[0034] (1) Add water to 100 parts of aspirin lignin to make a solution, adjust the pH to 10, raise the temperature to 70°C, add 20 parts of formaldehyde, and react for 1.5 hours to obtain hydroxymethylated lignin, then add sulfonating reagent 20 parts of sodium bisulfite were reacted for 1 hour to obtain sulfomethylated lignin, and then 20 parts of cationization reagent 2,3-epoxypropyltrimethylammonium chloride were added to react for 4 hours, and the reacted solution was transferred to Purified by dialysis in a dialysis bag with a molecular weight cut-off of 1000 for 6 days, rotary steamed, and freeze-dried to obtain amphoteric lignin;
[0035] (2) Dissolve the amphoteric lignin in water, add a sodium hydroxide solution with a mass concentration of 20%, adjust the pH of the amphoteric lignin solution to 7, make the surface of the amphoteric lignin neg...
Embodiment 3
[0039]A preparation method of a lignin-based antitumor drug carrier of the present invention comprises the following steps:
[0040] (1) Add water to 100 parts of wheatgrass lignin to make a solution, adjust the pH to 9, raise the temperature to 60°C, add 30 parts of formaldehyde, and react for 3 hours to obtain hydroxymethylated lignin, then add 30 parts of concentrated sulfuric acid as a sulfonating agent Parts, reacted for 2 hours to obtain sulfomethylated lignin, then added 35 parts of cationic reagent N-dimethylazetidine chloride and reacted for 3 hours, and transferred the reacted solution to a dialysis bag with a molecular weight cut off of 2000 Purified by medium dialysis for 5 days, rotary steamed, and freeze-dried to obtain amphoteric lignin;
[0041] (2) Dissolve the amphoteric lignin in water, add a sodium carbonate solution with a mass concentration of 25%, adjust the pH of the amphoteric lignin solution to 8, make its surface negatively charged, and then add the ...
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