Composite preservative containing natural essential oil nanoliposomes and application of composite preservative in preservation of sturgeon slices
A nanoliposome, natural essential oil technology, applied in the preservation of meat/fish with chemicals, food ingredients as antimicrobial preservation, food science, etc. Oxidation, broad bacteriostatic effect, prolonged storage period effect
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Embodiment 1
[0034] Weigh 3.402g of gallic acid and 7.914g of n-octanol, so that the molar ratio of gallic acid and n-octanol is 1:2.5, weigh 0.905g of immobilized lipase IM-100 as an enzymatic reaction catalyst, and measure 50mL of deep eutectic solvent (choline chloride: glycerol = 1:2, molar ratio) as the reaction medium, placed in an oil bath at 50°C for 13h, and eluted by silica gel column chromatography, petroleum ether and ethyl acetate in a volume ratio of 6:1 as the mobile phase , the prepared product is GAC8.
Embodiment 2
[0036] The preparation steps of described natural essential oil nano liposome (EO-NPs-Lps) are as follows:
[0037] Get 4mL of natural tea tree essential oil (TEO) and add in the glass sample bottle ( figure 1(a)), 1200rpm magnetic stirring, heating at 150°C for 0.5h, stop heating, continue stirring, and naturally cool to room temperature; then use a PVDF microporous membrane with a pore size of 0.45 μm to filter, collect the filtrate, and obtain natural tea tree essential oil containing nanoparticles Nanoparticle liquid (TEO-NPs) ( figure 1 (b)), 4°C, store for later use.
[0038] The preparation of natural tea tree essential oil nano liposome (TEO-NPs-Lps): above-mentioned 36mg natural tea tree essential oil nano particle liquid (TEO-NPs) is dissolved in the ethanol (96%) of 12mL, and fully mixes with 60mg lecithin, Stir magnetically at room temperature until the two are fully miscible, and store in a refrigerator at 4°C overnight. Then the above system was 60°C, and the ...
Embodiment 3
[0040] A preservative composition, consisting of the following components: GAC8 (octyl gallate) 0.1mg / L, natural essential oil nano liposome (EO-NPs-Lps) concentration of 0.1mg / L, solvent is ultrapure water .
[0041] The application of this antiseptic preservative composition comprises the following steps:
[0042] (1) Mix in ultrapure water so that the final concentration of GAC8 is 0.1mg / L, and the concentration of natural essential oil nanoliposomes (EO-NPs-Lps) is 0.1mg / L (shake well at 25°C). The dissolution is accelerated in an ultrasonic environment, the ultrasonic power is 150W, and the frequency is 40KHz. After completely dissolving, vibrate and shake well to obtain a special composite preservative for fish.
[0043] (2) Deploying antistaling agent: add twice the weight of water to the special compound antistaling agent for fish to prepare the antistaling liquid.
[0044] (3) Put the fish meat to be treated into the above-mentioned fresh-keeping liquid, soak it at...
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