Vaccine universal vectors and preparation method and application thereof

A vaccine carrier and vaccine technology, applied in the field of vaccine universal carrier protein and its preparation, can solve the problems of weak particle uniformity, time-consuming, manpower and material resources, and affecting immunogenicity
CN112062862AActive Publication Date: 2020-12-11INST OF PROCESS ENG CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF PROCESS ENG CHINESE ACAD OF SCI
Publication Date
2020-12-11

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Abstract

The invention provides vaccine universal vectors HBc-S and HBc (1-183) -S which are constructed based on SpyCatcher / SpyTag and take hepatitis B virus core protein as a basis. SpyCatcher is displayed in a main immunodominance region of VLPs and can be subjected to an adhesion reaction with SpyTag at the amino terminal of epitope polypeptide, linear, annular and phosphorylated B cell epitopes SP aredisplayed on the surfaces of VLPs, and a series of HBc-S-P VLPs vaccines are prepared. Mice immunized with the vaccines can generate antibodies specific to the epitopes; and HBc-S-pTau 422 remarkablyimproves cognition and pathological changes of Alzheimer' S disease transgenic mice; HBc (1-183) -S induces Th1 type immunoreaction, and the HBc (1-183) -S-OVA vaccines are of a VLP structure, can activate and promote maturation of DCs, remarkably inhibit growth of tumors and prolong the lifetime of the tumors.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and relates to a universal vaccine carrier and its preparation method and application, in particular to a vaccine universal carrier protein fused and expressed by SpyCatcher protein and truncated hepatitis B virus core protein or full-length hepatitis B virus core protein, and its preparation method and application. Background technique

[0002] Hepatitis B virus core protein is the nucleocapsid structural protein of hepatitis B virus (Hepatitis B), which can self-assemble into virus-like particles (Virus-like particle, VLP) with a particle size of about 30nm, which can easily enter lymph nodes. HBc VLPs particles are moderate in size, easy to be absorbed non-specifically by antigen-presenting cells in the way of endocytosis or pinocytosis, have a strong adjuvant effect, can activate the immune system in a T-cell-dependent or T-cell-independent manner, and are rich in T helper (Thelper, Th) cell epit...

Claims

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