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Cyanobacterium sp.SCSIO-45682 as well as culture method and application thereof

A cultivation method, the technology of cyanobacteria, applied in the biological field, can solve the problems of polysaccharide production decline, enemy invasion, and difficulty in amplifying cultivation, so as to inhibit the invasion of enemy organisms, inhibit the pollution of enemy organisms, and improve the extracellular Effect of polysaccharide production

Active Publication Date: 2020-12-29
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] (1) Most of the algae strains with the potential to produce active polysaccharides are vulnerable to the invasion of harmful organisms during the outdoor open-type large-scale cultivation process
[0004] (2) Most algae strains with active polysaccharide production potential are difficult to scale up and cultivate in outdoor open ponds because they do not have a wide range of extreme environmental adaptability
[0016] (1) Most of the algae strains with active polysaccharide production potential are vulnerable to the invasion of harmful organisms during the outdoor open pond cultivation process
[0017] (2) Most of the algae strains with the potential to produce active polysaccharides do not have a wide range of extreme environmental adaptability, grow slowly under extreme culture conditions, and are difficult to accumulate high biomass, so they are not suitable for outdoor large-scale cultivation
[0018] (3) Most of the algae strains with the potential to produce active polysaccharides have decreased polysaccharide production under extreme culture conditions, and cannot meet the requirements of the extraction process for the content of active substances in algae powder or wet algae mud

Method used

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  • Cyanobacterium sp.SCSIO-45682 as well as culture method and application thereof
  • Cyanobacterium sp.SCSIO-45682 as well as culture method and application thereof
  • Cyanobacterium sp.SCSIO-45682 as well as culture method and application thereof

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Experimental program
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Embodiment 1

[0050] 1. Identification of algae strains:

[0051] The algal strains were sampled from Tianya Town, Sanya City, Hainan Province (E109°19′38″, N18°18′31″). Single algae colonies were obtained by the plate separation method (f / 2 solid medium).

[0052] Morphological features:

[0053] The algae is unicellular, light blue-green, oval cylindrical, without flagella, 2-3 μm in length, 1-2 μm in diameter, in a free or binary state ( figure 1 ).

[0054] Molecular Biology Identification:

[0055] Extract the DNA of the algae, PCR amplify to obtain 16S rDNA and ITS rDNA fragments and then sequence the genes, and use the maximum similarity algorithm to construct the phylogenetic evolutionary tree ( figure 2 ), the measured ITS rDNA sequence (its sequence is shown in SEQ ID NO.2) adopts the maximum similarity algorithm to construct the phylogenetic tree ( image 3 ), this algal strain has a very high sequence similarity with Cyanobacterium aponinum PCC 10605 (BLAST alignment resul...

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Abstract

The invention discloses Cyanobacterium sp.SCSIO-45682 as well as a culture method and application thereof. A preservation number of the Cyanobacterium sp.SCSIO-45682 disclosed by the invention is CGMCC No.19697. The Cyanobacterium sp.SCSIO-45682 can be cultured under the conditions of high alkali, high salt and high pH, produces intracellular polysaccharides and extracellular polysaccharides, andcan be used as a production raw material of cyanobacterial polysaccharides.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a cyanobacteria and its cultivation method and application. Background technique [0002] Cyanobacteria have simple structure, fast reproduction rate, and strong environmental adaptability. They can accumulate a variety of biologically active substances, such as polysaccharides, phycocyanin, zeaxanthin, and β-carotene. Among them, cyanobacterial polysaccharides, as a widely used biologically active substance, have attracted increasing attention. Because of its anti-radiation, anti-virus, immune regulation, anti-tumor, anti-oxidation, metal ion chelation and other biological activities and excellent rheological properties, as well as biocompatibility, biodegradability and other natural biological macromolecules With excellent properties, cyanobacterial polysaccharides have wide application potential in cosmetics, skin care products and other fields. However, the current ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/01
CPCC12N1/20C12P19/04C12R2001/01C12N1/205
Inventor 向文洲陈子硕李涛吴华莲谭丽吴后波何慧
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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