Aminoglycoside antibiotic detection method based on fluorescent metal organic framework material
A metal-organic framework and aminoglycoside technology, applied in the field of chemical detection, can solve the problems of weakened fluorescence and poor water stability, and achieve the effects of simple operation, high sensitivity and rapid response
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Embodiment 1
[0037] Embodiment 1 Zn-TCPE aqueous solution preparation
[0038] Weigh 5 mg of Zn-TCPE and dissolve it in 5 mL of deionized water to obtain a 1 mg / mL Zn-TCPE aqueous solution, which is stored at room temperature in the dark. SEM image and fluorescence microscope image of Zn-TCPE before adding aqueous solution figure 1 As shown, the SEM image and fluorescence microscope image of Zn-TCPE after adding the aqueous solution are as follows: figure 2 As shown, it is proved that the crystal structure of Zn-TCPE in aqueous solution is cracked and the fluorescence is weakened. The fluorescence stability of Zn-TCPE aqueous solution after standing for 3h is as follows: image 3 shown.
Embodiment 2
[0039] Embodiment 2 draws tobramycin concentration standard curve
[0040] A certain mass of tobramycin standard substance was weighed and dissolved in deionized water to obtain a 0.5-10 mM tobramycin solution. Turn on the fluorescence spectrophotometer, set the excitation wavelength to 370nm, the excitation and emission slits to 5nm, and the voltage to 700V, take 1mL Zn-TCPE aqueous solution in a cuvette, measure the fluorescence value, and record it as the blank fluorescence value I 0 . Then add 1 μL of tobramycin solution, mix well, measure the fluorescence value, record it as fluorescence value I.
[0041] According to the fluorescence spectrum measured by the fluorescence spectrophotometer, fit the corresponding standard curve, such as Figure 4shown. The ratio of fluorescence value increases with the increase of tobramycin concentration, and its linear regression equation is y=0.5038x+0.9716, R 2 =0.9991, where y represents the fluorescence value I after adding tobra...
Embodiment 3
[0043] Embodiment 3 draws gentamicin sulfate concentration standard curve
[0044] A certain quality of gentamicin sulfate standard substance was weighed and dissolved in deionized water to obtain a 0.5-10 mM gentamicin sulfate solution. Turn on the fluorescence spectrophotometer, set the excitation wavelength to 370nm, the excitation and emission slits to 5nm, and the voltage to 700V, take 1mL Zn-TCPE aqueous solution in a cuvette, measure the fluorescence value, and record it as the blank fluorescence value I 0 . Then add 1 μL of gentamicin sulfate solution, mix well, measure the fluorescence value, record it as fluorescence value I.
[0045] According to the fluorescence spectrum measured by the fluorescence spectrophotometer, fit the corresponding standard curve, such as Figure 10 shown. Fluorescence value ratio increases with the increase of gentamicin sulfate concentration, and its linear regression equation is y=0.3213x+0.9939, R 2 =0.9973, where y represents the f...
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