A water-soluble rhodamine-based fluorescence/colorimetric dual-mode probe and its preparation method and application
A dual-mode probe, water-soluble technology, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of no accurate pH application range, inability to use biological samples for detection, cumbersome sample preparation process, etc., to achieve High practical application value, good water solubility and biocompatibility, and the effect of simplifying the preparation process
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Embodiment 1
[0049] A method for preparing a water-soluble rhodamine-based fluorescence / colorimetric dual-mode probe, comprising steps:
[0050] Dissolve 0.958g rhodamine 6G in 30mL ethanol, add 3.0g hydrazine hydrate aqueous solution (mass concentration: 85%) dropwise at room temperature, and complete the dropwise addition in 10 minutes, then increase the system temperature at 78°C, stir and reflux for 12h, stop Heat and cool to room temperature, filter to obtain a solid intermediate product, wash the solid intermediate product with ethanol / diethyl ether (volume ratio 1:1), and dry it in a vacuum desiccator to obtain rhodamine 6G hydrazide;
[0051] Dissolve 8g of polyethylene glycol 4000 and 0.9g of 4-formylbenzoic acid in 50mL of tetrahydrofuran, under nitrogen atmosphere, under the action of 0.09g of dicyclohexyldiimine and 1.64g of 4-dimethylaminopyridine catalyst, at room temperature After reacting for 24 hours, the obtained reaction solution was filtered to remove the precipitate, a...
Embodiment 2
[0056] A method for preparing a water-soluble rhodamine-based fluorescence / colorimetric dual-mode probe, comprising steps:
[0057] Dissolve 0.958g rhodamine 6G in 30mL ethanol, add 3.0g hydrazine hydrate aqueous solution (mass concentration: 85%) dropwise at room temperature, and complete the dropwise addition in 10 minutes, then increase the system temperature at 78°C, stir and reflux for 12h, stop Heat and cool to room temperature, filter to obtain a solid intermediate product, wash the solid intermediate product with ethanol / diethyl ether (volume ratio 1:1), and dry it in a vacuum desiccator to obtain rhodamine 6G hydrazide;
[0058] Dissolve 4g polyethylene glycol 2000 and 0.9g 4-formylbenzoic acid in 50mL tetrahydrofuran, under nitrogen atmosphere, under the action of 0.09g dicyclohexyldiimine and 1.64g 4-dimethylaminopyridine catalyst, room temperature After reacting for 24 hours, the obtained reaction solution was filtered to remove the precipitate, and the obtained fi...
Embodiment 3
[0061] A method for preparing a water-soluble rhodamine-based fluorescence / colorimetric dual-mode probe, comprising steps:
[0062] Dissolve 0.958g of rhodamine 6G in 30mL of ethanol, drop in 10.0g of an aqueous solution of hydrazine hydrate (mass concentration: 50%) at room temperature, and finish adding dropwise in 10 minutes, then increase the temperature of the system at 78°C, stir and reflux for 12 hours, stop Heat and cool to room temperature, filter to obtain a solid intermediate product, wash the solid intermediate product with ethanol / diethyl ether (volume ratio 1:1), and dry it in a vacuum desiccator to obtain rhodamine 6G hydrazide;
[0063] Dissolve 4g polyethylene glycol 2000 and 0.9g 4-formylbenzoic acid in 50mL tetrahydrofuran, under nitrogen atmosphere, under the action of 0.09g dicyclohexyldiimine and 1.64g 4-dimethylaminopyridine catalyst, room temperature After reacting for 24 hours, the obtained reaction solution was filtered to remove the precipitate, and ...
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