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Saline-alkaline-tolerant phosphate solubilizing bacterium and application thereof

A phosphate-solubilizing bacteria and saline-alkali-tolerant technology, applied in the fields of application, bacteria, biochemical equipment and methods, etc., can solve the problems of uneven phosphate-solubilizing efficiency, inability to tolerate high-alkali environment, and different application effects, etc. The effect of good dephosphorization effect

Pending Publication Date: 2021-01-05
山西大地生态环境技术研究院有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The reported phosphate-solubilizing bacteria are mainly concentrated in Bacillus, Pseudomonas, Serratia, Agrobacterium, Arthrobacter, etc. Phosphorus-solubilizing efficiency varies between plants, and the mechanism of phosphorus-solubilizing is different. The application effect in saline-alkali soil is different. Some phosphorus-solubilizing bacteria can only tolerate high-salt environment, but cannot tolerate high-alkaline environment. Affected by high alkali environment, it cannot tolerate high salinity environment, especially in the actual application process of saline-alkali land, affected by the complex field environment, the effect of phosphorus solubilizing bacteria is greatly reduced compared with the greenhouse environment

Method used

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  • Saline-alkaline-tolerant phosphate solubilizing bacterium and application thereof
  • Saline-alkaline-tolerant phosphate solubilizing bacterium and application thereof
  • Saline-alkaline-tolerant phosphate solubilizing bacterium and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1: Isolation and Identification of Phosphobacteria DC-3-P Strain

[0039] (1) The DC-3-P bacterial strain among the present invention adopts the enrichment culture method to obtain from Heilongjiang saline-alkali soil, and concrete steps are as follows:

[0040] (1) Inorganic phosphorus liquid medium is as follows: 10g glucose, 0.5g yeast powder, 0.5g (NH 4 ) 2 SO 4 , 0.3g NaCl, 0.3g KCl, 0.3g MgSO 4 ·7H 2 O, 0.03g FeSO 4 ·7H 2 O, 0.03g MnSO 4 4H 2 O, 5g Ca 3 (PO 4 ) 2 , add distilled water to 1000mL, pH 7.0-7.5.

[0041] (2) Acclimatization process: under aseptic operation, add 1 g of Heilongjiang saline-alkali soil to 100 mL of sterilized inorganic phosphorus medium, shake and cultivate in a shaker at 30 °C and 150 r / min for 7 days, and take 5 mL Bacteria liquid is inoculated in 100mL new inorganic phosphorus liquid medium, and the pH of inorganic phosphorus liquid medium increases according to 0.5 unit, namely 7.0, 7.5, 8.0, 8.5, 9.0, 9.5, changes...

Embodiment 2

[0059] Embodiment 2: Physiological and biochemical characteristics of Bacillus megaterium DC-3-P bacterial strain

[0060] (1) Starch hydrolysis test

[0061] Culture medium preparation: add soluble starch to the broth medium composed of beef extract 3g / L, peptone 10g / / L, and NaCl 5g / L, so that the final concentration in the medium is 0.2%, add 2% agar , sterilized at 121°C for 20 minutes, poured into a plate under aseptic operation and set aside. Lugol's iodine solution: 1g iodine, 2g potassium iodide, first dissolve potassium iodide with a small amount of water, then add iodine tablets, after the iodine is completely dissolved, add water to dilute to 300ml. Spot the bacterial strains on a plate and culture at 30°C for 2-4 days. After colonies are formed, add Lugol's iodine solution dropwise on the plate to cover the colonies. Positive reaction (starch is hydrolyzed) is that the agar medium is dark blue, and a colorless transparent circle appears around the colony or cultur...

Embodiment 3

[0078] Example 3: Determination of Phosphorus Solubilizing Efficiency of Phosphorus Solubilizing Bacteria DC-3-P under Different Saline-Alkali Stress Conditions

[0079] At pH 7.0, temperature 30°C, the salinity of the inorganic phosphorus liquid medium is pH=7.0, NaCl concentration 0.5%, pH=7.5, NaCl concentration 1.0%, pH=8.0, NaCl concentration 2.0%, pH=8.5, NaCl concentration 3.0%, pH=9.0, NaCl concentration 4.0%, pH=9.5, NaCl concentration 5.0%, the culture of DC-3-P strain was inoculated in 100mL above-mentioned different saline-alkali stress with tricalcium phosphate as the sole phosphorus source In the inorganic phosphorus liquid culture medium, measure the phosphorus content in the medium after shaking culture at 150r / min for 3 days, take different culture conditions as the abscissa, and take the dissolved phosphorus concentration as the ordinate. The results are shown in figure 1 .

[0080] It can be seen from the figure that with the strengthening of saline-alkali ...

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Abstract

The invention relates to the technical field of agricultural microorganisms, in particular to a saline-alkaline-tolerant phosphate solubilizing bacterium and application thereof in improvement of saline-alkaline soil. The phosphate solubilizing bacterium is bacillus megaterium, and the preservation number of the phosphate solubilizing bacterium is CCTCC M 2020453. The phosphate solubilizing bacterium can tolerate saline-alkali conditions of pH as high as 9.5 and 5.0% NaCl, has a high phosphate solubilizing function, and is especially suitable for development of biological bacterial fertilizersof phosphate solubilizing bacteria in saline-alkaline soil.

Description

technical field [0001] The invention relates to the technical field of saline-alkali land improvement, in particular to a saline-alkali-tolerant phosphorus-solubilizing bacteria and its application in saline-alkali land. Background technique [0002] Phosphorus is one of the mineral elements necessary for plant growth and development. It is mainly used for the synthesis of nucleic acids, phospholipids and ATP during plant growth, and promotes crop growth through photosynthesis, biological oxidation, nutrient absorption and cellular catabolism. . More than 95% of the phosphorus in the soil exists as inactive phosphorus such as organic phosphorus or insoluble inorganic phosphorus, which cannot be used by plants. Phosphate-Solubilizing Bacteria is a kind of growth-promoting bacteria that can convert insoluble phosphorus into phosphorus that can be absorbed and utilized by plants. It plays an important role in improving the content of available phosphorus in soil. A hot researc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/80C12R1/11
CPCC12N1/20C05D1/00C05G3/80C12R2001/11C12N1/205C05D3/02C05F5/002C05F11/00C05F11/08
Inventor 王瑾杨成立雷涛李婷婷彭湃杨君子
Owner 山西大地生态环境技术研究院有限公司
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