Lactobacillus plantarum capable of relieving diarrhea caused by ETEC and application of lactobacillus plantarum

A technology of Lactobacillus plantarum and diarrhea, which is applied in the field of microbiology and can solve problems such as uncertain effects

Active Publication Date: 2021-01-19
无锡特殊食品与营养健康研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has been reported in the literature that Lactobacillus plantarum can relieve diarrhea caused by ETEC, the quality of the effect is still uncertain.

Method used

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  • Lactobacillus plantarum capable of relieving diarrhea caused by ETEC and application of lactobacillus plantarum
  • Lactobacillus plantarum capable of relieving diarrhea caused by ETEC and application of lactobacillus plantarum
  • Lactobacillus plantarum capable of relieving diarrhea caused by ETEC and application of lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Example 1: Screening and strain identification of Lactobacillus plantarum CCFM1143

[0074] 1. Screening

[0075] Taking Huadian kimchi from Zhaodong, Heilongjiang as a sample, take 0.5g of the sample preserved in 30% (v / v) glycerol and add it to a 10mL centrifuge tube containing 4.5mL of normal saline under a sterile environment to obtain 10 -1 Diluent, repeat the above dilution steps to obtain 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Dilution: draw 100 μL of gradient dilutions of different gradients and spread them on the MRS solid medium, incubate at 37°C for 72 hours to obtain a diluted coating plate; pick typical colonies on the diluted coating plate and inoculate them into the MRS liquid medium respectively , Cultivate at 37°C for 48h to obtain the bacterial liquid.

[0076] 2. Identification

[0077] After numbering each bacterial strain corresponding to each bacterial liquid of separation and purification, the steps recorded in the textbook "Microbiology" (Shen ...

Embodiment 2

[0086] Example 2: Effects of Lactobacillus plantarum CCFM1143 on body weight and feces water content of mice with diarrhea

[0087] Specific steps are as follows:

[0088]40 BALB / c mice were taken and fed for 1 week under the conditions of room temperature 22-24°C, humidity 40-60%, 12h / 12h day and night, free access to food and water, and then randomly divided into 5 groups. Group 8, 5 groups are: control group, model group, Brady's yeast group (CNCMI-745 group) fed with Brady's yeast liquid, QHLJZD13L6 group with Lactobacillus plantarum (Lactobacillus plantarum) QHLJZD13L6 bacterial liquid group, and the CCFM1143 group fed with Lactobacillus plantarum (Lactobacillus plantarum) CCFM1143 bacterial solution.

[0089] The experiment lasted for 3 weeks: the experiment started after 1 week of adaptive feeding of the animals. From 7 days before modeling, until the end of the experiment (excluding the 3 days of antibiotic treatment), the mice in the control group and the model grou...

Embodiment 3

[0094] Example 3: Effect of Lactobacillus plantarum CCFM1143 on pathological damage of jejunum in diarrhea mice

[0095] Specific steps are as follows:

[0096] 40 BALB / c mice were taken and fed for 1 week under the conditions of room temperature 22-24°C, humidity 40-60%, 12h / 12h day and night, free access to food and water, and then randomly divided into 5 groups. Group 8, 5 groups are: control group, model group, Brady's yeast group (CNCMI-745 group) fed with Brady's yeast liquid, QHLJZD13L6 group with Lactobacillus plantarum (Lactobacillus plantarum) QHLJZD13L6 bacterial liquid group, and the CCFM1143 group fed with Lactobacillus plantarum (Lactobacillus plantarum) CCFM1143 bacterial solution.

[0097] The experiment lasted for 3 weeks: the experiment started after 1 week of adaptive feeding of the animals. From 7 days before modeling, until the end of the experiment (excluding the 3 days of antibiotic treatment), the mice in the control group and the model group were fed...

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Abstract

The invention discloses lactobacillus plantarum capable of relieving diarrhea caused by ETEC and an application of the lactobacillus plantarum, and belongs to the technical field of microorganisms. According to the invention, lactobacillus plantarum CCFM1143 is screened out, and the strain has the effect of relieving diarrhea; The effect of the lactobacillus plantarum is specifically embodied in the following aspects: the weight of a diarrhea mouse is remarkably increased, the excrement water content of the diarrhea mouse is remarkably reduced, the pathological injury of the jejunum of the diarrhea mouse is remarkably relieved, the inflammatory factor level in the serum of the diarrhea mouse is remarkably reduced, the content of enterotoxin ST in the serum of the diarrhea mouse is remarkably reduced, the content of aquaporin AQP3 in the serum of the diarrhea mouse is remarkably increased, the abundance of intestinal flora of the diarrhea mouse is remarkably increased, and the content of short chain fatty acids in the diarrhea mouse is remarkably increased. Compared with lactobacillus plantarum reported in other literatures, the lactobacillus plantarum provided by the invention hasbetter capability of reducing TNF and IL-6 levels and reducing crypt depth, and has a huge application prospect in preparation of products (such as foods or medicines) for preventing and / or treating diarrhea.

Description

technical field [0001] The invention relates to a strain of lactobacillus plantarum capable of relieving diarrhea caused by ETEC and an application thereof, belonging to the technical field of microbes. Background technique [0002] ETEC, enterotoxic Escherichia coli, settles on the surface of the small intestine, does not damage or invade the epithelial cells of the intestinal mucosa, and causes secretory diarrhea by producing enterotoxin. Helicobacter is one of the main pathogens of "tourist's diarrhea" in developed countries, a common cause of "adult cholera syndrome", and an important pathogen of children's diarrhea. Its incidence rate is second only to rotavirus. [0003] At the same time, ETEC is also an important pathogen that causes diarrhea in domestic animals, especially young animals (newborn piglets, calves, etc.). After newborn piglets are infected with ETEC, they often die due to severe watery diarrhea and rapid dehydration. The morbidity and mortality are hig...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23L33/135A23L29/00A61K35/747A61P1/12C12R1/25
CPCC12N1/20A23C9/1234A23L33/135A23L29/065A61K35/747A61P1/12A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/32A23V2200/30
Inventor 陈卫杨波岳月何竹筠赵建新张灏
Owner 无锡特殊食品与营养健康研究院有限公司
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