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Probiotic composition, method for producing probiotic composition and use thereof

A manufacturing method and technology of probiotics, applied in the field of reproduction, can solve the problems of increasing feed cost, time-consuming and labor-intensive, etc., and achieve the effects of reducing the use of antibiotics and drugs, adjusting intestinal function, and enhancing immunity

Pending Publication Date: 2021-01-19
NAT KAOHSIUNG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 2. The problem of high temperature resistance of probiotics: During the production process of feed, it must be heated to at least 80°C or even above 120°C. Therefore, if you want to add probiotics to the feed, there are currently two methods. One is to induce probiotics Form endospores and then enter the production process, but only a few strains can achieve this. Second, after the feed is made, the probiotics are sprayed on the feed, but this is time-consuming and labor-intensive, and will increase the cost of the feed

Method used

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  • Probiotic composition, method for producing probiotic composition and use thereof
  • Probiotic composition, method for producing probiotic composition and use thereof
  • Probiotic composition, method for producing probiotic composition and use thereof

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Experimental program
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Effect test

Embodiment 1

[0034] The invention provides a method for producing a probiotic composition. The Saccharomyces boulardii bacteria liquid is mixed with a sodium alginate solution, wherein the weight ratio of sodium alginate to the sodium alginate solution is 1% to 10%, and calcium ions are solid The weight ratio of the substance to the calcium ion solution is 0.5% to 5%. The bacteria solution is mixed with the sodium alginate solution at a weight percentage of 2:1, and is continuously added dropwise to the calcium ion solution until it is fixed and shaped, and carried out Drying program.

Embodiment 2

[0036] The invention provides a method for producing a probiotic composition, which comprises mixing Pandora bacteria, lactic acid bacteria and yeast Saccharomyces boulardii with a sodium alginate solution, mixing the three bacteria solutions with the alginic acid solution in 1 : 3% by weight mixed, in addition to add the energy-producing nutrients (mineral phosphorus) and other non-nutrient substances (animal extracts, such as fish meal, meat and bone meal, scallop extract) required for farming, and continuously add them dropwise to the calcium ion solution Until it is fixed and shaped, it can also be further dried.

[0037] In the manufacturing method of the probiotic composition of the present invention, the weight ratio of sodium alginate or alginic acid in the solution is 1%-10%, preferably 1%-6%, most optimally 1%-5%.

[0038] In the manufacturing method of the probiotic composition of the present invention, the weight ratio of calcium ion solids in the calcium ion solutio...

Embodiment 3

[0053] In the present invention, the 3wt% sodium alginate solution and the probiotic liquid for aquatic products are uniformly mixed in a ratio of 1:2 by weight, and the probiotic liquid is such as: Shewanella, Pandora, Pseudomonas, lactic acid bacteria, bismuth Mycobacterium, yeast or bacillus, etc. are dropped into 2.5wt% calcium chloride solution to make it fixed and shaped, then dried, and then added to the general feed process to make aquaculture feed.

[0054] experimental method

[0055] Shewanella bacteria culture

[0056] (1) Drug preparation:

[0057] Experimental drugs: LB medium, agar (Agar), sodium alginate, calcium chloride, PBS (10X) pH 7.4

[0058] LB medium: Prepare 10g / L tryptone (Tryptone), 5g / L yeast powder (Yeasextract) and 5g / L sodium chloride (NaCl) with primary water.

[0059] (2) Experimental steps:

[0060] Pick a single colony of Shewanella and put it into 5ml LB medium, shake and culture at 37°C for 16 hours, pour 5ml into 1LLB medium, shake and...

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Abstract

The present invention discloses a method for producing a probiotic composition, including: providing a bacterial cell suspension, which is one or more bacterial cell suspensions of a bacterium or Saccharomyces boulardii; mixing the bacterial cell suspension with a sodium alginate solution or an alginic acid solution; and adding the mixture to a calcium ion solution until the mixture is immobilizedin a shape. The technology of the present invention has the effects of long-term preservation at room temperature and resistance to high temperature, and can be applied to ordinary bacterial strains,without being limited to a small number of bacterial species able to form endospore, and without requiring the strains to be frozen for preservation. The method of the present invention can be applied to the preparation of aquatic feeds, animal feeds, or probiotics that human beings need.

Description

technical field [0001] The present invention relates to a method of propagating, maintaining or preserving microorganisms or compositions thereof, in particular to preserving or maintaining living microorganisms; to forming or establishing an animal feed, especially by agglomeration; by making grain forming. Background technique [0002] A lactic acid bacterium and its use, which can maintain growth in a culture environment, contribute to the growth and immunity of fish, and provide a lactic acid probiotic for aquaculture, which uses lactic acid probiotics to enhance the growth of cultured organisms Growth and immunity to improve the harvest of farmed fish, but there are still practical limitations. Since probiotics cannot withstand high temperatures, it is impossible to directly put probiotic strains into the feed, and it must be done after the feed is manufactured. Spraying, spraying probiotics on the surface of the feed, but this is time-consuming and labor-intensive, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/04A23K10/18A23K40/10A23K50/80A23L33/135A23P10/20A61K35/741A61K36/064A61K35/744A61K35/745A61K35/742A61K9/16A61K47/36A61K47/02A61P37/04A61P1/00
CPCC12N11/10C12N11/04A23K10/18A23K40/10A23K50/80A23L33/135A23P10/20A61K35/741A61K36/064A61K35/744A61K35/745A61K35/742A61K9/1652A61K9/1611A61P37/04A61P1/00A61K2300/00A23K20/26A23K20/20A23K20/174A61K35/74A23V2002/00C12N1/20A23K30/00A61K39/07A61K39/09A61K39/104A61K2035/115
Inventor 黄胤唐郑令虹
Owner NAT KAOHSIUNG UNIV OF SCI & TECH