Unlock instant, AI-driven research and patent intelligence for your innovation.

Trichoderma filamentous fungus mutant strain and method for producing protein

A manufacturing method and technology of filamentous bacteria, applied in the field of mutant strains of Trichoderma filamentous bacteria, can solve the problems of reduced production of cellulase and reduced production of cellulase, and achieve increased specific activity and improved manufacturing capacity Effect

Pending Publication Date: 2021-01-19
TORAY IND INC
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the other hand, it is also known that gene modification reduces the production amount of cellulase. Non-Patent Document 2 describes the effect of using lactose or cellulose as an inducer when the sugar transporter of Trichoderma reesei is deficient. Reduced production of cellulase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Trichoderma filamentous fungus mutant strain and method for producing protein
  • Trichoderma filamentous fungus mutant strain and method for producing protein
  • Trichoderma filamentous fungus mutant strain and method for producing protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0056] The following examples are given to describe the present invention in detail.

reference example 1

[0057] Method for Measuring Protein Concentration

[0058] A protein concentration measurement reagent (Quick Start Bradford Protein Assay, manufactured by Bio-Rad) was used. 5 μL of the diluted filamentous bacteria culture solution was added to 250 μL of the protein concentration measurement reagent returned to room temperature, and the absorbance at 595 nm after standing at room temperature for 5 minutes was measured with a microplate reader. Using BSA as a standard, the protein concentration was calculated from the standard curve.

reference example 2

[0059] Method for Measuring the Specific Activity of Cellulase

[0060] (Method for Determination of Specific Activity of β-Glucosidase)

[0061] 10 μL of the enzyme dilution solution was added to 90 μL of 50 mM acetate buffer solution containing 1 mM p-nitrophenyl-β-glucopyranoside (manufactured by Sigma Aldrich Japan Co., Ltd.), and reacted at 30° C. for 10 minutes. Then 10 μL of 2M sodium carbonate was added and mixed well to stop the reaction, and the increase in absorbance at 405 nm was measured. The specific activity was calculated by dividing the activity of 1 μmol of p-nitrophenol freed per minute as 1 U, and dividing this by the amount of protein.

[0062] (Method for Determination of β-Xylosidase Specific Activity)

[0063] 10 μL of the enzyme dilution solution was added to 90 μL of 50 mM acetate buffer solution containing 1 mM p-nitrophenyl-β-xylopyranoside (manufactured by Sigma Aldrich Japan Co., Ltd.), and reacted at 30° C. for 30 minutes. Then, 10 µL of 2M s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to: a Trichoderma filamentous fungus mutant strain having reduced functionality of a polypeptide formed by an amino acid sequence of SEQ ID NO: 2; and a high-producing method for a protein by using the mutant strain.

Description

technical field [0001] The present invention relates to a mutant strain of a filamentous fungus belonging to the genus Trichoderma having improved protein production ability, and a method for producing a protein using the mutant strain. Background technique [0002] Filamentous fungi belonging to the genus Trichoderma are known to have a high protein-producing ability, and studies have been conducted so far on production of proteins using the same filamentous fungi. The filamentous fungus belonging to the genus Trichoderma uses cellulose, lactose, cellobiose, etc. as an inducer, and produces, among proteins, cellulase classified as a saccharification enzyme. [0003] In order to further increase the production amount of cellulase, in particular, to enhance the production amount of β-glucosidase which accounts for a low proportion of glucoamylase, studies using genetic modification have been conducted. Among them, overexpression and deletion of factors controlling cellulase ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/15C12N9/42C12N15/09C12P19/14C12P21/02
CPCC12P21/02C12P19/14C12N15/80C07K14/37C12R2001/885C12N9/2437C12P19/02C12P21/00C12Y302/01004
Inventor 加川雄介平松绅吾山田胜成
Owner TORAY IND INC
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com