128results about How to "Reduce mutation" patented technology

The invention aims at the technical defects of off-target effects of an existing CRISPR / Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR associated protein 9) gene editing technology to carry out site-directed mutation of an amino acid site on a site combined with a DNA (Deoxyribonucleic Acid) sequence of Cas9 protein to obtain the Cas9 protein with low off-target efficiency,so as to realize a gene editing unction with higher targeting performance and higher efficiency. Meanwhile, the invention further provides a method for carrying out HEK293T cell gene editing by adopting a gene editing method.

The present disclosure provides human renal stem cells. Also described are human renal stem cells isolated from the papillary region of the human kidney and methods of isolating the same. Also described are methods for culturing, characterizing, and differentiating the same, including methods for identifying human renal stem cells that are positive for Nestin and CD133, and methods for allowing the cells to differentiate into neurons.

The present invention describes a methodology for generating high fidelity PCR products, and also cloning of such high fidelity PCR products in a suitable vector. Generation of polymerase-induced mutant fraction of target sequences during PCR amplification is linearly proportional to the number of doublings of the target sequences. Thus the high fidelity PCR products are generated by minimizing the number of doublings of the target nucleic acid sequences during PCR amplification. Minimization of number of doublings of the target sequences is achieved by reducing the number of cycles of PCR amplification of the target sequences. The high fidelity PCR products thus obtained are then cloned into a suitable vector. As an example, a 960 bp target sequence from E. coli DNA was PCR-amplified only for 3 cycles, and it was then directly cloned into a positive selection cloning vector pRGR2Ap. The functional analysis of the inserts in all clones showed that the clones carried functionally wild-type DNA fragments, and hence the inserts most probably carry no mutation. Cloning of PCR products obtained from 3 cycles of amplification, instead of 30 cycles of amplification, theoretically achieves 10-fold reduction of mutations in the cloned fragments. The invention also contemplates cloning of a target cDNA obtained by primer extension.

The invention discloses a touch panel and a touch display device, and the touch panel comprises a first type of touch electrodes which are connected with a first signal line and a second signal line;between the first type of touch electrodes and the driving module, the second signal line comprises a first part and a second part which are insulated from each other; A first part of a second signalline is connected in parallel with a first signal line connected with a first type of touch electrodes, the abrupt change of the first signal lines connected with the two adjacent touch electrodes canbe effectively relieved, so that the problem of non-uniform display of each area of the touch panel can be relieved, and in addition, by floating the second parts of the second signal lines, interference between the adjacent first signal lines can be effectively isolated.

The invention relates to an enteromorpha polysaccharide oral liquid and a preparation method thereof. The invention is characterized by adopting the following technical scheme: 1, obtaining enteromorpha polysaccharide by using enteromorpha as a raw material through purification, drying, pulverizing, extracting with hot water, enzymolysis, enzyme deactivation, centrifuging purification, ethanol precipitation and vacuum drying in sequence; 2, weighing 30-40% of lentinula edodes, 10-30% of kelp, 10-30% of yams and 10-30% of Chinese wolfberries in percentage by weight, and preparing a Chinese medicinal herb extract for later use through procedures of mixing, pulverizing, extracting in hot water, centrifuging, concentration, vacuum drying and the like; and 3, mixing 5-15% of enteromorpha polysaccharide, 5-15% of Chinese medicinal herb extract, 5-10% of sweetening agents and the balance of water in percentage by weight, and sterilizing, bottling, sealing, inspecting and packing the mixed liquid to obtain a finished product. The enteromorpha polysaccharide oral liquid is rich in nutrition and high in safety, and has multiple health-care functions of reducing blood sugar, reducing blood fat, enhancing the immunity, resisting tumors and the like.

Methods and engineered yeast cells for generating a benzylisoquinoline alkaloid product are provided herein. A method comprises providing engineered yeast cells and a feedstock to a reactor. In the reactor, the engineered yeast cells are subjected to fermentation by incubating the engineered yeast cells for a time period to produce a solution comprising the BIA product and cellular material. The solution comprises not more than one class of molecule selected from the group of protoberberine, morphinan, isopavine, aporphine, and benzylisoquinoline. Additionally, at least one separation unit is used to separate the BIA product from the cellular material to provide the product stream comprising the BIA product.

The invention provides an SOE-PCR method capable of effectively connecting multiple DNA fragments. In the method, completely complementary splicing area primers are adopted, non-symmetrically weighting primers (i.e. the molar ratio of the primers positioned at two tail ends of a target-length fragment and the primer positioned on the target-length fragment splicing area is 40-120:1) are introduced during overlap extension, thus realizing the purpose that finishes connection of multiple fragments is finished in the same reaction. Meanwhile, the invention also has the advantages that template amount is not required to optimize, and amplification specificity is high.

The invention relates to a VEGFR-2 resistant monoclonal antibody with human and mouse cross reaction as well as a preparation method and an application thereof. The antibody is generated by the secretion of a hybridoma cell strain 8H1, has human and mouse cross reaction and recognizes three forms of a natural antigen VEGFR-2 in a high specificity way. The VEGFR-2 resistant monoclonal antibody A8H1with human and mouse cross reaction is generated by the secretion of the hybridoma cell strain 8H1 of a VEGFR-2 resistant monoclonal antibody. The preparation method comprises the following steps: preparing a mouse immunogen and an antigen for detection; preparing the VEGFR-2 resistant hybridoma cell strain and adopting His-VEGFR-2 recombinant protein as the immunogen to carry out immunity inoculation on hypodermic injection at the belly of a pure line BALB / c mouse; screening a VEGFR-2 positive monoclonal antibody and screening the monoclonal antibody with positive VEGFR-2 and negative normalhuman serum by an ELISA method; authenticating the subtype of the monoclonal antibody; producing the monoclonal antibody in the BALB / c mouse and purifying; and carrying out function authentication and application on A8H1 by methods of ELISA, and the like.

A hydrogen manufacturing system for performing offgas flow control includes: a vaporizer (1) for heating a material mixture containing a hydrocarbon material; a reforming reactor (2) for generating hydrogen-containing reformed gas by reforming reactions of the material; a PSA separator (5) for repeating a cycle of adsorption and desorption, where in the adsorption PSA separation is performed with an adsorption tower loaded with an adsorbent to adsorb unnecessary components in the reformed gas and extract hydrogen-enriched gas out of the tower, and in the desorption the offgas containing the unnecessary components from the adsorbent and remaining hydrogen is discharged from the tower; and a buffer tank (6) for holding the offgas before supplying to the vaporizer. The offgas flow supply from the tank (6) to the vaporizer is changed continuously over time when the cycle time is changed according to load change on the separator (5).

The invention provides a heating method and device, a heating appliance and a computer readable storage medium. The heating control method is applicable to the heating appliance, wherein the heating appliance is provided with an infrared heating assembly and an electromagnetic heating assembly. The heating control method comprises the steps of distributing a target heating power value according topreset power value distribution rules so as to determine a first target heating power value and a second target heating power value; controlling the infrared heating assembly to heat in a first preset mode according to the first target heating power value; and controlling the electromagnetic heating assembly to heat in a second preset mode according to the second target heating power value. The technical scheme of the invention effectively reduces the occurrence of a phenomenon that the total heating power exceeds the rated power of household electrical appliances due to the simultaneous electromagnetic heating and infrared heating. In addition, low-power heating can be realized, and the safety performance and user experience of the heating appliance.

The present invention provides methods of treating hepatitis virus infection. The methods generally involve administering a composition comprising an antiviral agent in a dosing regimen that achieves a multiphasic serum concentration profile of the antiviral agent. The dosing regiment includes dosing events that are less frequent than with currently available hepatitis therapies. The multiphasic antiviral agent serum concentration profile that is achieved using the methods of the invention effects an initial rapid drop in viral titer, followed by a further decrease in viral titer over time, to achieve a sustained viral response

The invention provides an effective method for reducing impact current of inverters in grid connection so that the inverters can switch to the grid-connected operating state from the independent operating state easily and effectively. According to the mechanism, if the voltages on the two sides of a grid connection switch have the same amplitude and phase in grid connection, voltage difference does not exist, and thus impact current cannot be generated; in independent operation, inversion voltage becomes higher and higher, impact on filter capacitors is reduced, and in grid connection, the voltages on the two sides of the grid connection switch are consistent, and generation of impact current is suppressed; after grid connection, because double-loop control is adopted and only a given current loop is switched, sudden change of current can be effectively reduced, and smooth transition is achieved.

Methods of treating a mammal that is deficient in CD4+ and / or CD8+ lymphocytes are provided. The methods comprise inoculating the mammal with an attenuated mycobacterium in the M. tuberculosis complex. In these methods, the mycobacterium comprises two deletions, wherein a virulent mycobacterium in the M. tuberculosis complex having either deletion exhibits attenuated virulence. Use of these mycobacteria for the manufacture of a medicament for the treatment of mammals deficient in CD4+ and / or CD8+ lymphocytes is also provided.

The invention provides a segmented video processing method and device, a terminal and a storage medium. The method comprises the steps of obtaining two segmented videos to be synthesized; Respectivelyacquiring video frames to be superposed from the two segmented videos according to a set time length and the synthesis sequence of the two segmented videos; Performing corresponding superposition onthe video frames to be superposed in the two segmented videos to obtain superposed video frames; And synthesizing the two segmented videos and the superposed video frame according to the synthesis sequence to obtain a target video. According to the method and the device, when the two segmented videos are synthesized, part of video frames between the two segmented videos are correspondingly superposed, so that the transition effect of the synthesized video is improved, and picture abrupt change is reduced.

The invention discloses a centrifugal compressor with inlet guide blades and a turbocharger. The centrifugal compressor comprises a volute, an impeller and guide blades, wherein an air inlet channel, an impeller mounting space and an air outlet channel are defined in the volute, the impeller mounting space is respectively communicated with the air inlet channel and the air outlet channel, and the air inlet channel is provided with an air inlet and the air outlet channel is provided with an air outlet; the impeller can be rotationally arranged in the impeller mounting space by surrounding around a revolving shaft and is provided with a plurality of blades; and guide blades are arranged on a peripheral wall of the air inlet channel. According to the centrifugal compressor provided by the invention, the peripheral speed of fluid such as air in the air inlet channel is reduced by the fact that the guide blades are mounted, so that air inflows at the tips of the blades can be more uniform; and moreover, and the sudden change of the peripheral speed can be reduced, so that the increasing ranges of thermal charges at the tips of the blades can be reduced, the property of the centrifugal compressor can be further improved, especially, the property can be remarkably improved under the small flow working condition.

The invention relates to an electromagnetic valve combination-based cooling wind tunnel temperature control method. According to the method, firstly, a cooling control system is used for realizing thecooling function through extruding and pushing the liquid nitrogen. The control of the injection flow is realized through controlling a switch combination of an electromagnetic valve arranged on a liquid collecting ring, and then the accurate temperature control is realized. The liquid collecting ring is provided with a liquid nitrogen injection type electromagnetic valve and a nozzle used for filling the liquid nitrogen at the same time. An electromagnetic valve component on the liquid collecting ring is composed of an upstream electromagnetic valve set and a downstream electromagnetic valveset. The upstream electromagnetic valve set is located between a compressor and a heat exchanger. The distance between the upstream electromagnetic valve set and the tail end of the compressor is about 2 meters. The distance between the downstream electromagnetic valve set and the tail end of the compressor is about 4 meters. The nozzle is arranged at the tail end of the electromagnetic valve. The liquid nitrogen injection of the corresponding flow of the nozzle is realized by controlling an electromagnetic valve switch. According to the invention, through controlling the switch combination of the electromagnetic valve, the flow rate and the injection position of an injected refrigerant are controlled. Therefore, the cooling control of the wind tunnel is realized. The method has the advantages of high control precision, good temperature uniformity, simple control and good operability.

The invention belongs to the technical field of feed, and concretely discloses a feed for breeding black goats and a producing method thereof. The breeding feed mainly comprises an energy feed, a feed additive, a mineral matter feed and a mildew inhibitor according to certain parts by weight, and the feed is prepared by mixed fermentation; wherein the energy feed is mainly prepared from corn flour, wheat bran, bean dregs, rice bran, alfalfa, rye grass and Draco-cephalum moldavica according to certain parts by weight; the feed additive is mainly prepared by mulberry barks, radix stephaniae tetrandrae, human placenta, officinal magnolia barks, Taxillus sutchuenensis, vladimiria roots, Grifola frondosa, licorice, rehmannia roots, and Chinese wolfberry fruits according to certain parts by weight; the mineral matter feed is mainly prepared by shell powder, bone meal, sodium bicarbonate, and salt according to certain parts by weight. The breeding feed has green and safe raw materials, and is rich in cellulose, protein, mineral matter, Chinese herbal medicine components for improving immunity and a natural mildew inhibitor with abundant and comprehensive nutrition and without toxic and side effects and residuals; the feed can be used for effectively improving resistibility and growth speed of black goats.

The invention relates to the technical field of a power semiconductor device, in particular to a superjunction power device. In the superjunction power device provided by the invention, each second conductive type of semiconductor post in a first conductive type of semiconductor drift region has two or more than two different widths (a1, a2 until an, and n is more than or equal to 2), and the distance between two adjacent second conductive types of semiconductor posts has two or more than two different widths (b1, b2 until bm, and m is more than or equal to 2). The corresponding drain-source voltage points which are consumed by the superjunction post and have different widths and distances are different, thus, abruptly-reduced source-drain voltage span of a miller capacitor Cgd and a source-drain capacitor Cds is increased, abrupt reduction of the Cgd and the Coss is relieved, and the current-voltage oscillation is reduced.

The invention belongs to the technical field of semiconductor power devices, and particularly relates to a semiconductor super junction power device. The device comprises a terminal region and a cellular region. The cellular region comprises a drain region in a substrate epitaxial layer, a JFET region, and multiple vertical parallel columnar doped regions. The top of each columnar doped region is provided with a body region. Each body region is internally provided with source regions. A gate oxide layer is arranged on the body regions and the JFET region. Gates are arranged on the gate oxide layer. There are two or more kinds of spacing of different width between the adjacent columnar doped regions, and the body regions have two or more kinds of different width. Under the synergistic action of the columnar doped region structures with different spacing and the body region structures of different width, more slow change can be introduced to the super junction power device, gate-drain capacitance mutation becomes smoother, and gate turbulence caused by gate-drain capacitance mutation is reduced.

Host cells that are engineered to produce benzylisoquinoline alkaloid (BIAs) precursors, such as norcoclaurine (NC) and norlaudanosoline (NL), are provided. The host cells may have one or more engineered modifications selected from: a feedback inhibition alleviating mutation in a enzyme gene; a transcriptional modulation modification of a biosynthetic enzyme gene; an inactivating mutation in an enzyme; and a heterologous coding sequence. Also provided are methods of producing a BIA of interest or a precursor thereof using the host cells and compositions, e.g., kits, systems etc., that find use in methods of the invention.

The invention discloses a precise real-time controllable fiber paving compaction device which is provided with a compaction roller driven by a servo motor and transmitted by a ball screw to move front and back. The compaction roller acts through a spring to achieve driven movement in the front-and-back direction. When carbon fibers are conveyed to the die surface through a carbon fiber composite material fiber paving head from the position below the compaction roller, the compaction roller is controlled to be in contact with the die surface, and presoaking yarns and a die are attacked and cured through the compaction roller. Compaction force of the compaction roller is obtained from spring deformation amount, a laser type displacement sensor is used for measuring the spring deformation amount, the spring deformation amount is fed back to a controller, control is achieved through a PID unit degeneration control method, the compaction servo motor is controlled to drive the ball screw to rotate, the spring compression amount is adjusted, and the compaction roller outputs constant compaction force. The precise real-time controllable fiber paving compaction device is simple in control manner, the machining cost is reduced, the compaction force suddenly-changed condition can be avoided, stress is more even, and precise real-time controllable fiber paving compaction device is suitable for carbon fiber paving at any angle.

Host cells that are engineered to produce benzylisoquinoline alkaloid (BIAs) precursors, such as norcodaurine (NC) and norlaudanosoline (NL), are provided. The host cells may have one or more engineered modifications selected from: a feedback inhibition alleviating mutation in a enzyme gene; a transcriptional modulation modification of a biosynthetic enzyme gene; an inactivating mutation in an enzyme; and a heterologous coding sequence. Also provided are methods of producing a BIA of interest or a precursor thereof using the host cells and compositions, e.g., kits, systems etc., that find use in methods of the invention.

The invention relates to a low-noise diversion volute of a range hood. The low-noise diversion volute comprises a volute body, an impeller is mounted in the volute body, and the upper outlet of the volute shell is connected with the range hood through a flange; the volute body comprises a volute plate which is enclosed to form an annular diversion space, and the inversion space is provided with a notch; volute protecting plates are respectively mounted on axial sides of the diversion space, each volute protecting plate is provided with an air inlet, and upper ends of the volute protecting plates and the notch end of the volute plate are enclosed to form an outlet; a curved-surface diversion structure is mounted on the outer side of the volute plate and comprises a diversion plate and supporting plates symmetrically located at two ends of the diversion plate, and two sides of the supporting plates and two sides of the diversion plate are respectively fixedly connected between the volute protecting plates. Airflow resistance of the range hood is effectively reduced through combination of guidance of the curved-surface diversion structure and guidance of the circular-arc surfaced volute plate, the situation of turbulent flow is improved, and flow-induced noise outside the volute and aerodynamic noise of the impeller are reduced.

The invention discloses an inverter power supply current limiting control method of a variable load current feedforward coefficient. The inverter power supply current limiting control method comprises the following steps: (1) judging whether an inverter power supply is short-circuited or not; if so, entering step (4); otherwise, entering step (2); (2) judging whether a load current effective value is more than three times of rated load current or not; if so, entering step (5); otherwise, entering step (3); (3) judging whether the load current effective value is more than two times of the rated load current or not; if so, entering step (6); otherwise, entering step (7); (4) setting a feedforward coefficient to be 0 and finishing; (5) setting the feedforward coefficient to be 0 and entering step (8); (6) modifying the feedforward coefficient and entering step (8); (7) setting the feedforward coefficient to be 1 and entering step (8); (8) carrying out filtering processing on the feedforward coefficient. The method provided by the invention has the beneficial effect of improving output waveform quality of the inverter power supply under complicated conditions including an overload or short-circuit condition and the like.

The invention provides methods and devices for controlling synchronous pre-synchronization and synchronization detection closing of a microgrid. The control method comprises the steps of: adjusting the voltage magnitude output by a main support power supply at a tracking side through iteration, so that the voltage magnitude at the tracking side successively approximates the voltage magnitude at areference side along with iteration till the absolute value of a difference value between the voltage magnitude at the tracking side and the voltage magnitude at the reference side is less than or equal to a first pre-set threshold value; adjusting the frequency output by the main support power supply at the tracking side through iteration, so that the frequency at the tracking side successively approximates the frequency at the reference side along with iteration till the absolute value of a different value between the frequency at the tracking side and the frequency at the reference side isless than or equal to a second preset threshold value; and, adjusting the phase output by the main support power supply at the tracking side through iteration, so that the phase at the tracking side successively approximates the phase at the reference side along with iteration till the absolute value of a different value between the phase at the tracking side and the phase at the reference side isless than or equal to a third preset threshold value, therefore, voltage synchronization of the tracking side and the reference side is realized.

The invention discloses a welding-with-trailing impact rolling welded seam shaping device for a welded joint. The welding-with-trailing impact rolling welded seam shaping device comprises a vibrationtelescopic device, and further comprises a main rolling wheel and an auxiliary rolling wheel which are arranged at the bottom of the vibration telescopic device and located on the same horizontal plane; the main rolling wheel comprises a cylinder body and bowl-shaped bosses symmetrically arranged at the two ends of the cylinder body, a plurality of punching protruding patterns are evenly arrangedon the outer surface of the cylinder body at intervals along the circumference, and a first pin through hole is formed in the center of the cylinder body and the center of the bowl-shaped bosses; andin the horizontal direction of the cylinder body along the bowl-shaped bosses, the outer diameters of the bowl-shaped bosses are gradually decreased, and the outer diameters of the edges of the ends,away from the cylinder body, of the bowl-shaped bosses are slightly greater than the outer diameter of the cylinder body. The high-temperature welded seam after welding are treated by the structure ofthe welding-with-trailing impact rolling welded seam shaping device, the organization structure of the welded seam can be improved, the welding quality of the welded joint and the welded seam is improved, and thus the service life of a weldment is prolonged. The welding-with-trailing impact rolling welded seam shaping device is easy to operate and simple in structure, a control system can be additionally arranged to achieve automation, and manpower is saved.

The invention relates to the technical field of medical epidemic prevention, in particular to a high-temperature anti-virus mask accessory comprising a heat insulator, a main valve and a filtering piece which are sequentially arranged in a shell at intervals from the inside to the outside of a mask; a high-temperature sterilization cavity is formed between the main valve and the filtering piece; aheating body is arranged in the high-temperature sterilization cavity; the heating body is electrically connected with a storage battery; a temperature adjusting potentiometer is electrically connected between the heating body and the storage battery; and a plurality of partition plates are arranged on the side surface of the heating body in the high-temperature sterilization cavity at intervals.According to the invention, exhaust gas of patients with diseases is subjected to high-temperature virus killing; for ordinary people, sucked gas is sterilized at high temperature and thus the safetyof people is guaranteed; various labyrinth cooling energy-saving channel structures are adopted, so that the situation that the part of air is not sterilized is avoided and thus the sterilization effect is improved; the multi-stage cooling effect is achieved, intervals of different temperatures are isolated, and temperature streaming is prevented.

Methods for modifying a dystrophin gene are disclosed, for restoring dystrophin expression within a cell having an endogenous frameshift or nonsense mutation within the dystrophin gene. The methods comprise introducing a first cut within an exon en or intron of the dystrophin gene creating a first exon end or intron end, wherein said first cut is located upstream of the endogenous frameshift or nonsense mutation; and introducing a second cut within an exon or intron of the dystrophin gene creating a second exon end or intron end, wherein said second cut is located downstream of the frameshift or nonsense mutation. Upon joining / ligation of said first and second exon ends or intron ends a hybrid exon or intron junction is created and dystrophin expression is restored, as the correct reading frame is restored. Reagents and uses of the method are also disclosed, for example to treat a subject suffering from muscular dystrophy.

The invention discloses a stable shearing tension force setting method which is applicable to a circle shear specification changing process of a sour rolling mill unit. The method includes selecting N steel coils and collecting characteristic parameters; importing an allowance adjustment coefficient alpha of a tension force setting value; setting serial numbers of the steel coils; calculating a front average tension force setting value and a rear average tension force setting value of circle shears of stripes of the steel coils according to a circle shear tension force setting model; setting a front total tension force setting value and a rear total tension force setting value of the circle shears; acquiring a front tension force changing rate and a rear tension force changing rate, and judging whether a front total tension force setting value and a rear total tension force setting value are required to be recalculated according to the front tension force changing rate and the rear tension force changing rate; performing repeated calculation until front total tension force setting values and rear total tension force setting values of all the N steel coils are calculated; and respectively setting front total shearing tension forces and rear total shearing tension forces of the N steel coils in the shearing process of the circle shears according to the calculated front total tension force setting values and the rear total tension force setting values.