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Isolated lung mechanical perfusion fluid and its preparation method and application

A perfusate and lung technology, which is applied to the field of isolated lung mechanical perfusate and its preparation, can solve the problems of single source of Steen solution, effective treatment of pneumonia, limited perfusion repair, etc., so as to improve the qualified rate of lung donors and reduce oxidative damage. , the effect of reducing the cost of preparation

Active Publication Date: 2022-06-07
WUXI PEOPLES HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mechanical perfusion time of Steen solution on the isolated lung before transplantation is mostly about 4 hours. The lack of perfusion time limits the perfusion repair of the isolated donor lung by EVLP technology. Some diseases with relatively long treatment time, such as pneumonia caused by infection, are difficult to Get effective treatment during perfusion
The source of Steen fluid is single and must rely on imports. Its price is very high, and the price of perfusion fluid will bring an unbearable economic burden to patients

Method used

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  • Isolated lung mechanical perfusion fluid and its preparation method and application
  • Isolated lung mechanical perfusion fluid and its preparation method and application
  • Isolated lung mechanical perfusion fluid and its preparation method and application

Examples

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preparation example Construction

[0049] An embodiment of the present invention also provides a method for preparing the above-mentioned isolated lung mechanical perfusate, including the following steps S10-S20.

[0050] Step S10: Provide raw materials according to the components of the above-mentioned isolated lung mechanical perfusate.

[0051] Step S20: Mix dextran, albumin, glutamine, amino acid, sorbitol, glucose and inorganic salt with sterile water for injection.

[0052] In some embodiments, dextran, glutamine, amino acids, sorbitol, glucose and inorganic salts are respectively dissolved in sterile water for injection, or a plurality of components are mixed and dissolved, albumin is dissolved in physiological saline for injection, filtered, Configure into different solutions, and mix the different solutions configured.

[0053] In some of the preferred embodiments, step S20 includes the following steps S21-S29:

[0054] Step S21: Dissolve dextran and glucose with sterile water for injection, and filt...

Embodiment 1

[0076] (1) Preparation of isolated lung mechanical perfusate

[0077] 1. Dextran-40 glucose solution: take 30 g of dextran with a molecular weight of about 40 kDa and 25 g of anhydrous glucose, dissolve it in sterile water for injection to 500 mL, and filter at 0.22 μm to obtain;

[0078] 2. Human albumin solution: take 80g of human albumin with a molecular weight of about 66kDa, dissolve it to 400mL in sterile water for injection, and filter it at 0.22μm;

[0079] 3. Sodium chloride solution: take 9g of sodium chloride, dissolve it in sterile water for injection to 1000mL, and filter at 0.22μm to obtain;

[0080] 4. Calcium chloride solution: take 0.5 g of anhydrous calcium chloride, dissolve it in sterile water for injection to 10 mL, and filter it with 0.22 μm to get it;

[0081] 5. Compound potassium and magnesium aspartate solution: take 0.452 g of anhydrous potassium aspartate and 0.4 g of anhydrous magnesium aspartate, dissolve them in sterile water for injection to 10...

Embodiment 2

[0106] The difference between this example and Example 1 is that the concentrations of components contained in the isolated lung mechanical perfusate are different, as shown in Table 3, and other steps and process parameters are basically the same as those in Example 1.

[0107] table 3

[0108] component Concentrationg / L Human albumin solution 75 Dextran-40 2.4 Glucose solution 2.0 Glutamine solution 0.2 amino acid 0.2 Sorbitol 0.3

[0109] The isolated lung mechanical perfusion solution prepared in Example 2 was used for in vitro perfusion repair of the lung, which could be maintained for 11 hours, and no new edema, hematoma or emphysema appeared in the lung.

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Abstract

The invention relates to an isolated lung mechanical perfusion fluid and its preparation method and application. The isolated lung mechanical perfusion fluid includes dextran, mammalian albumin, inorganic salts, glutamine, amino acids, sorbitol and glucose. The isolated lung mechanical perfusion solution can improve the energy metabolism of the isolated lung, maintain a good gas exchange function within 12 hours without causing damage to the isolated lung. In addition, the isolated lung mechanical perfusion fluid of the present invention can significantly reduce the consumption of the isolated lung mechanical perfusion fluid during actual use, and when used with the electrolyte replacement fluid, only 2L of the isolated lung mechanical perfusion fluid is consumed during the 12h EVLP process. On the basis of cost reduction, the implementation time of EVLP can be effectively extended, and the storage time of isolated lungs can be improved.

Description

technical field [0001] The invention relates to the technical field of organ preservation, in particular to an isolated lung mechanical perfusate and a preparation method and application thereof. Background technique [0002] As lung transplantation technology continues to mature, lung transplantation has become the standard treatment for end-stage lung diseases that cannot be cured by drugs or surgery. However, the number of patients on the waiting list for lung transplantation is increasing year by year, and the shortage of transplantable lungs is becoming more and more serious. The utilization rate of donor lungs is only about 5%, which can only meet the needs of a very small number of people for lung transplantation. [0003] The reason for the shortage of qualified donor lungs is that most of the donor lungs provided to the transplant team have lung damage or related pulmonary complications before the death of the donor, and are not fully suitable for transplantation. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0247
Inventor 陈静瑜卢艳卫栋林祥华刘峰刘强钟嘉伟杨振坤张勃毛禹康周鹏林冠金
Owner WUXI PEOPLES HOSPITAL
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