Fluorescent quantitative PCR (Polymerase Chain Reaction) testing device

Abstract

The invention discloses a fluorescent quantitative PCR (Polymerase Chain Reaction) testing device which is used for improving the heating uniformity of a sample and the accuracy of a detection result,and belongs to the technical field of biological devices. The device comprises a base and supporting legs, wherein a heating bin and a sample bin are arranged above the base, an air inlet is formed in the side face of the heating bin, an electric heating wire, a temperature sensor and an induced draft fan are arranged in the heating bin, the sample bin communicates with the heating bin through anair cavity, a plurality of exhaust holes are evenly formed in the side wall face of the air cavity, a sample holder is arranged in the sample bin, a plurality of test tube holes are formed in the sample holder, a bottom plate is further arranged at the bottom of the sample bin, supporting grooves are formed in the positions, corresponding to the test tube holes, of the bottom plate, and an exhaust fan and heat dissipation holes are arranged on the back face of the sample bin. According to the fluorescent quantitative PCR testing device, the sample bin can be uniformly filled with hot air frommultiple angles and positions to heat samples, so that the samples are heated more uniformly, and the accuracy of the detection result is guaranteed.

Description

technical field [0001] A fluorescent quantitative PCR test device is used for improving the uniformity of sample heating and the accuracy of detection results, and belongs to the technical field of biological devices. Background technique [0002] The fluorescent quantitative PCR instrument is composed of a fluorescent quantitative system and a computer. The PCR instrument is a gene amplification instrument. PCR is to use DNA polymerase to carry out chain reaction replication and amplification of the genes in the enzyme reaction tube. Through the clinical application of this instrument, the Early rapid and accurate detection, tracking methods that can be used for chemotherapy to treat cancer, etc., to achieve the purpose of early diagnosis and timely treatment of patients. [0003] In the process of fluorescence detection, the temperature requirements are high, and the temperature heating error and uneven heating of the sample will affect the accuracy and accuracy of the det...

AI Technical Summary

Problems solved by technology

[0003] In the process of fluorescence detection, the temperature requirements are high, and the temperature heating error and uneven heating of the sample will affect the accuracy and accuracy of the detection results. Most PCR instruments are heated by setting electric heating wires at the bottom of the sample heating chamber. This causes the bottom of the sample test tube to heat up rapidly, while the temperature of the upper sample rises slowly, which makes the sample heated very unevenly, and may also destroy the reaction of the sample detection solution; in addition, after the detection and analysis are completed, the internal temperature of the sample heating chamber cannot be cooled quickly. It is inconvenient for the staff to take out the test tube, which will slow down the test process and reduce the test efficiency

Images