A method for determining organophosphorus flame retardants in plants
An organophosphorus and flame retardant technology, which is applied in the field of determination of organophosphorus flame retardants in plants, can solve the problems of limited analysis and determination methods of OPFRs, and achieve the effects of low detection limit, good separation effect and accurate purification treatment.
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[0032] 1. Determination and analysis method
[0033] (1) The extraction pool and all glass instruments were cleaned with three organic solvents, acetone, dichloromethane and n-hexane. The plant samples are washed, freeze-dried, ground and pulverized.
[0034] (2) Weigh 1g of the crushed plant sample, mix it with 1g of diatomaceous earth (60-80 mesh), put it into a 33mL extraction cell, add a recovery rate indicator (100ng d 12 -TCEP, d 27 -TNBP and TAP), using a fast solvent extractor to extract the extract. The extraction conditions are: extraction temperature: 80°C; extraction solvent: dichloromethane / acetone (volume ratio: 1:1); extraction pressure: 10MPa; heat balance time: 5min; static extraction time: 5min; rinse volume: 40%; Purge time: 60s; number of cycles: 2; cleaning: on; solvent consumption: 35mL.
[0035] (3) Rotate and concentrate the extract, transfer it to a polytetrafluoroethylene centrifuge tube, add 5 g of ferric phosphate and 10 mL of n-hexane to remove...
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