Process for preparing L-tryptophan by fermentation method

A tryptophan and fermentation technology, applied in the field of amino acid fermentation, can solve the problems of difficult control of fermentation parameters and complex fermentation process, and achieve the effects of minimizing environmental pollution, simple separation process, and improving utilization rate

Pending Publication Date: 2021-03-02
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fermentation method adopted in this method improves the acid production efficiency, but there are problems such as relatively complicated fermentation process and difficult control of fermentation parameters.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A process for preparing L-tryptophan by fermentation, comprising the steps of:

[0027] Step 1) Fermentation of strains: Inoculate L-tryptophan-producing Escherichia coli (E.coliTRTH) seed solution into the fermentation medium with 10% inoculum, cultivate at 36°C, and pass air volume, stirring speed, tank pressure and flow Add sugar to control the dissolved oxygen to 20%, maintain the pH of the tank system at 6.8 by feeding liquid ammonia, and add defoaming agent to defoam; when the fermentation reaches 24 hours, add a glucose solution with a concentration of 500g / L into the tank. Maintain the sugar concentration in the tank at 0.5-1g / L until the end of the fermentation. The total fermentation time is 50 hours to obtain the L-tryptophan fermentation broth. After testing, the L-tryptophan content is 48.9g / L; the fermentation medium (150m³ / 350m³): glucose 20g / L, magnesium sulfate 6.8g / L, potassium dihydrogen phosphate 3g / L, ammonium sulfate 2.4g / L, ferrous sulfate 15mg / L,...

Embodiment 2

[0034] A process for preparing L-tryptophan by fermentation, comprising the steps of:

[0035] Step 1) Fermentation of strains: Inject L-tryptophan-producing Escherichia coli (E.coliTRTH) seed solution into the fermentation medium with a 10% inoculation amount, cultivate at a temperature of 35°C, and pass air volume, stirring speed, tank pressure and flow Add sugar to control the dissolved oxygen to 30%, maintain the pH of the tank system at 6.9 by feeding liquid ammonia, and add defoaming agent to defoam; when the fermentation reaches 24 hours, add a glucose solution with a concentration of 500g / L into the tank. Maintain the sugar concentration in the tank at 0.5-1g / L until the end of the fermentation. The total fermentation time is 50 hours to obtain the L-tryptophan fermentation broth. After testing, the L-tryptophan content is 48.9g / L; the fermentation medium (150m³ / 350m³): glucose 20g / L, magnesium sulfate 6.8g / L, potassium dihydrogen phosphate 3g / L, ammonium sulfate 2.4g / ...

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Abstract

The invention belongs to the technical field of amino acid fermentation, and discloses a process for preparing L-tryptophan by a fermentation method. The process comprises the following steps: step 1)strain fermentation, step 2) ceramic membrane filtration, step 3) chromatographic separation, step 4) decolorization, step 5) evaporation and concentration, and step 6) crystallization, centrifugation and drying. The process for preparing the L-tryptophan by the fermentation method is simple and efficient in production process, more convenient to operate and maintain, high in fermentation production efficiency and capable of being used for industrial continuous production.

Description

technical field [0001] The invention belongs to the field of amino acid fermentation, and in particular relates to a process for preparing L-tryptophan by fermentation. Background technique [0002] L-tryptophan, also known as α-aminoindole propionic acid, molecular formula: C11H12N2O2, it is the precursor of the important neurotransmitter 5-hydroxytryptamine in the human body, and it is one of the essential amino acids for the human body. It can be used as a nutritional supplement for pregnant women Special milk powder for infants and infants can also be used as a sedative to regulate mental rhythm and improve sleep. [0003] The earliest production of L-tryptophan mainly relied on chemical synthesis and protein hydrolysis. In recent years, there have also been studies on the production of tryptophan by direct fermentation and chemical synthesis, and the combination of direct fermentation and transformation. With the continuous development of research on the production of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/22C07D209/20C12N1/20C12R1/19
CPCC07D209/20C12N1/20C12P13/227
Inventor 张宗华冯世红崔小红边恩来王飞杨晓芳王奎智
Owner XINJIANG FUFENG BIOTECH
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