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Bitter inhibiting peptide and application thereof

A technology for a bitterness inhibitor and inhibitory peptide, which is applied to the bitterness inhibitory peptide and its application field, can solve the problems of high cost, less application and small application range, and achieves the effects of simple application operation, improved flavor and good water solubility.

Active Publication Date: 2021-03-05
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the use of ultrafiltration technology is also an effective method for debittering. Bitter peptides can be removed by using several ultrafiltration membranes with different molecular weight cut-off ranges, but the application range is small, and it is currently only used in soybean debittering
The enzymatic method uses exopolypeptidase to cut off the hydrophobic amino acids at both ends of the bitter peptide to achieve the purpose of debittering, but this method is costly and rarely used

Method used

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  • Bitter inhibiting peptide and application thereof
  • Bitter inhibiting peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Screening of Bioactive Peptides with Bitter Taste Inhibition

[0021] 1. Screening of bitter taste inhibitory peptides

[0022] The present invention uses ExPASy PeptideCutter (http: / / web.expasy.org / peptide_cutter / ), an online enzymolysis tool, to carry out virtual enzymolysis on the myosin sequence of Ezo scallop, and obtain 323 polypeptides after enzymolysis. The biological activity and water solubility of 323 peptides were screened by peptide property calculator and PeptideRanker online program. Toxicity prediction was performed using the ADMET program of DS2017 software, and three toxicity properties were predicted: mutagenicity, developmental toxicity and skin sensitization. We obtained 10 safe and non-toxic peptides with good water solubility, bioactivity score higher than 0.5, and their sequences were SM, RR, ADM, GFPSR, GIR, KPM, QRPR, NPPK, DM, DPDF.

[0023] 2. Prediction and analysis of bitter taste inhibitory activity

[0024] The crystal struc...

Embodiment 2

[0025] Example 2. Verification of Bitter Taste Inhibitory Peptide QRPR Inhibiting Bitter Taste Activity

[0026]Peptide QRPR was prepared by means of solid-phase synthesis, fermentation or enzymatic hydrolysis, and the freeze-dried powder of the peptide was dissolved in deionized water to prepare different solubility solutions, and 50mL of 1mM quinine solution was added to prepare the solution to be tested. The bitter taste inhibitory peptide LEGSLE was used as a positive control, and the solution was filled into a special beaker for the electronic tongue, and placed in an automatic sampling analysis device to detect changes in membrane potential. The results showed that the 0.15mg / mL active peptide QRPR had a 50% inhibitory rate on the bitter taste of quinine, and the inhibitory effect was stronger than that of the positive control.

[0027] At the same time, the bitterness inhibitory peptide QRPR can also be used as a bitterness inhibitor in foods composed of bitter ingredie...

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Abstract

The invention discloses application of a bitter inhibiting peptide Gln-Arg-Pro-Arg in preparation of a bitter inhibitor with a bitter covering action. The bioactive peptide Gln-Arg-Pro-Arg with a bitter inhibiting action provided by the invention can be conjugated with an active locus of a human bitter acceptor TAS2R14 and has a very good inhibiting effect on bitter; the peptide has the characteristics of safety, no toxic or side effects, good water solubility and the like; technical problems in the prior art that the bitter inhibiting effect is not obvious, and the like are solved; and the peptide can be applied to foods, healthcare products and medicines as the bitter inhibitor and has an extensive application prospect and an all-important significance.

Description

technical field [0001] The invention belongs to the field of biologically active peptides, and in particular relates to a bitter taste inhibiting peptide and its application. Background technique [0002] Taste is an extremely important sense in mammals. Bitterness is one of the five basic tastes, with an extremely low threshold and a longer duration than other tastes. Many natural organic substances include caffeine, nicotine, strychnine and many Chemically synthesized drugs are all bitter substances. A relatively mild bitter taste will cause unpleasant feelings, while a strong bitter taste will cause nausea, vomiting and psychological disgust. Many consumers will refuse to buy food products because of their own bitter taste. Studies have found that many functional ingredients in food, health products and medicines have bitter taste, for example, hops extract, a bitter component with sedative and gastric effects contained in beer; catechins and tannins contained in tea and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/113C07K14/435A23L29/00A23L5/20A61K47/18
CPCC07K5/1021C07K14/43504A23L29/045A23L5/27A61K47/183Y02A50/30
Inventor 励建荣李冬慧赵文竹于志鹏
Owner BOHAI UNIV