Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bacillus subtilis separated from bee bread and application thereof

A technology of Bacillus subtilis and bee food, which is applied in the field of Bacillus subtilis to prepare fermented bee pollen, can solve the problems of restricting the development and utilization of pollen, and destroying the extraction efficiency of active ingredients, so as to promote digestion, absorption and release, and improve the efficiency of bee pollen. Health benefits, effects of increasing the content of total phenols and total flavonoids

Active Publication Date: 2021-03-05
HUAZHONG AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bee pollen has an incomparably hard cell wall. The pollen cell wall is relatively tough, and has the physical and chemical properties of acid resistance, alkali resistance, temperature resistance, pressure resistance, and very stable to gastric acid and other digestive system enzymes. When the pollen directly enters the human digestive tract, Only a small part of nutrients can be released through the germination pores on the pollen surface, and the existence of the pollen wall limits the development and utilization of pollen
However, if the bee pollen is extracted by high temperature and high pressure, it is easy to destroy the active ingredients and the extraction efficiency is very low.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus subtilis separated from bee bread and application thereof
  • Bacillus subtilis separated from bee bread and application thereof
  • Bacillus subtilis separated from bee bread and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Separation and purification of microorganisms and identification

[0024] 1. Isolation and purification of microorganisms from bee bread

[0025] For aseptic operation, take 5g of bee grain, add 45ml of sterile water to a 150ml sterile Erlenmeyer flask, shake and mix well, and then gradiently dilute to an appropriate concentration. Draw 100 μl of the diluted sample solution of each concentration, put it into the LB solid medium, spread the bacterial solution evenly with a spreader, and cultivate it in a constant temperature incubator at 37°C for 20 hours.

[0026] Single colonies with different shapes were picked, streaked and inoculated on LB solid plates, and cultured in a constant temperature incubator at 37°C for 20 hours. Repeat the above operation and purify for 4-5 generations until pure colonies are obtained. The obtained single bacterial strain was stored at -80°C in a glycerol tube containing 50% LB liquid medium and 50% glycerol containing 40%.

...

Embodiment 2

[0055] Application of embodiment 2 microorganisms in fermented bee pollen

[0056] 1. Strain culture

[0057] The two strains of bacillus obtained by the above isolation and purification and another strain of bacillus licheniformis preserved in the laboratory were respectively cultivated, the method is as follows:

[0058] (1) Bacillus licheniformis: Pick 2 rings of Bacillus licheniformis from the solid-state plate, insert it into a 250ml Erlenmeyer flask containing 30ml LB medium, culture it at 37°C with shaking at 120r / min for 12h, and then inoculate it with 10% The amount was transferred into a 250ml Erlenmeyer flask containing 30ml LB medium, and cultured at 37°C with shaking at 120r / min for 10h.

[0059] (2) Bacillus subtilis: Pick 2 rings of Bacillus subtilis from the solid plate separated and purified above, insert it into a 250ml Erlenmeyer flask containing 30ml LB medium, culture at 37°C for 12h with shaking at 120r / min, and then 10% of the inoculum was transferred ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Bacillus subtilis XMFFL separated from bee bread, which is preserved in China center for type culture Collection with the preservation number of CCTCC NO: M 2020782. The strain has good fermentation capability on bee pollen and can convert and form more total phenols and total flavone active substances. The invention also discloses a preparation method of fermented bee pollen. The method can improve the total phenol content of the bee pollen to 2.19 times and the total flavone content to 1.72 times, thereby endowing the bee pollen with better health care efficacy.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, relates to a strain of Bacillus subtilis isolated from bee grain, and also relates to the application of the Bacillus subtilis in preparing fermented bee pollen. Background technique [0002] Bee food, also known as hive pollen and bee bread, is not only food for young bees and worker bees, but also a source of nutrition for larval development. Many species of Apiaceae have the habit of brewing bee food. Taking honey bees as an example, worker bees collect pollen balls and drop them in the nest chamber, and office bees chew, mix them with honey, and compact them. In the process, Pollen, bee secretions and honey are mixed into a chyle-shaped pollen mass, which is fermented by microorganisms in a beehive at a certain temperature and humidity to make peak grains. Among them, the microbial fermentation and brewing of pollen mass is the key to the formation of bee food, and it is also the determ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L21/20A23L33/00C12R1/125
CPCA23L21/20A23L33/00A23V2002/00A23V2200/30Y02P60/87
Inventor 刘睿张会芳唐翠娥鲁群
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com