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Bacterial strain for degrading high-ring polycyclic aromatic hydrocarbon and application of bacterial strain in pollution remediation

A technology of polycyclic aromatic hydrocarbons and bacterial strains, applied in the restoration of polluted soil, water pollutants, contaminated groundwater/leachate treatment, etc., can solve few problems and achieve good degradation effect

Inactive Publication Date: 2021-03-12
北京博诚立新环境科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, most degrading bacteria can grow with pyrene (tetracyclic) as the only carbon source and energy source, and there are few relevant literature reports on single degrading bacteria using benzopyrene (pentacyclic) as the sole carbon source and energy source.

Method used

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  • Bacterial strain for degrading high-ring polycyclic aromatic hydrocarbon and application of bacterial strain in pollution remediation
  • Bacterial strain for degrading high-ring polycyclic aromatic hydrocarbon and application of bacterial strain in pollution remediation
  • Bacterial strain for degrading high-ring polycyclic aromatic hydrocarbon and application of bacterial strain in pollution remediation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023]Example 1: Enrichment, purification, screening and identification of strains of the invention

[0024](1) inorganic salt medium

[0025]NA2HPO42.44G, KH2PO41.52G, (NH4)2SO40.5g, MGSO4 0.2g, CaCL2 0.05g, FESO42mg, Znso410 mg, deionized water 1000ml, pH = 7.0-7.2, 121 ° C sterilized for 20 min.

[0026](2) Enrichment culture, purification of strains

[0027]Take 10G Shandong 's coking plant polluted soil samples, add inorganic salt medium at 1: 9, add 0.1% Tween-80, pH = 7.0, 30 ° C constant temperature culture, and the shaker rotation speed is 160 r / min, and cultured for a week. Subsequently, the inorganic salt medium added to the addition of benzene (A) pyrene, the initial concentration of benzo (A) was 5 mg / L, multiple transition, benzo (A) pyrene concentration to 20 mg / L.

[0028](3) Separation and purification of strains

[0029]The enriched culture solution is diluted into a suitable gradient, coating the flat plate detection, and selects the advantageous single bacteria to transfer t...

Embodiment 2

[0044]Example 2: Application of strain BS-1 in multi-ring aromatic hydrocarbon weight pollution soil repair

[0045]This test is collected in a coke plant in Shandong. After the collection of soil samples are naturally air-dried, impurities such as stone, branches are removed, and 2 mm sample sieves. After pulverizing> 2mm earth block, after 2 mm sample sieve, mix, standby.

[0046]The bacterial suspension was prepared: the strain was transferred to an inorganic salt medium added to the addition of benzene [A] pyrene (10 mg / L), and cultured from 48 to 72 h. The cultured bacterial liquid was centrifuged for 10 min to collect the bacteria, and 3 were washed 3 times with physiological saline.

[0047]Try 10 g of experiment soil into a 150 mL conical bottle, add 40 ml of foundation inorganic salt medium, 0.1% Tween-80. Press 104The amount of CFU / mL inoculated into the experimental soil, pH = 7.0, 30 ° C constant temperature culture, the shaker rotation speed was 160 r / min, and 14d was cult...

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Abstract

The invention provides a bacterial strain for degrading high-ring polycyclic aromatic hydrocarbon, which belongs to microbacterium, has a preservation name of Microbacterium sp.BS-1, is preserved in China General Microbiological Culture Collection Center, and has a preservation number of CGMCC No.18248. The biological characteristics are gram-positive bacteria, bacterial colonies are yellow, circular and convex, and the edges are tidy, smooth and moist. The invention further provides an application of the strain for degrading the high-ring polycyclic aromatic hydrocarbon and a surfactant in remediation of polycyclic aromatic hydrocarbon contaminated soil or underground water in a synergistic mode. The strain Microbacterium sp. BS-1 has the capacity of efficiently degrading high-ring polycyclic aromatic hydrocarbon (including pyrene and benzo[a]pyrene), can be used for repairing polycyclic aromatic hydrocarbon pollution, has broad spectrum for degrading polycyclic aromatic hydrocarbon,also has a better degradation effect for actual polycyclic aromatic hydrocarbon polluted soil, and does not need to add other co-metabolic carbon sources.

Description

Technical field[0001]The present invention relates to the field of biodegradation treatment, in particular, in particular, to a strain of degradation of high-ring polycyclic aromatic hydrocarbons and its contamination repair applications.Background technique[0002]Polycyclic aromatic hydrocarbons are an aromatic hydrocarbon containing two or more benzene rings, with three tromothenesis - carcinogenic, teratogenic, and mutation. The world is released from about 43,000 tons of polycyclic aromatic hydrocarbons in the world, and 230,000 tons enter the marine environment. Due to its higher lipophilicity, multi-ring aromatic hydrocarbons in the environment is easily dispensed into organisms, and enters the human body through the food chain to constitute a great threat to human health and ecological environments.[0003]The polycyclic aromatic hydrocarbon compound consisting of four benzene rings is a high cyclic polycyclic aromatic hydrocarbon. Benzoxyl [a] is a polycyclic aromatic hydrocarb...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/32C02F103/06
CPCB09C1/10C02F3/34C02F2101/327C02F2103/06
Inventor 陈波洋牟新涛刘婉君
Owner 北京博诚立新环境科技股份有限公司
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