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Double-electrode photoelectrochemical immunosensor, preparation method and application thereof

An immunosensor and photoelectrochemical technology, applied in scientific instruments, instruments, and material analysis through electromagnetic means, can solve the problems of low detection sensitivity and weak current signal, and achieve high sensitivity, low cost, and light detection of target antigen Ag. Significant effect of current signal response

Active Publication Date: 2021-03-16
江苏知至生物科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

Nevertheless, the detection sensitivity of the photocathode sensing category is significantly lower than that of the photoanodic sensing category due to the fact that the current signal output by the photocathode is generally weaker.

Method used

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  • Double-electrode photoelectrochemical immunosensor, preparation method and application thereof
  • Double-electrode photoelectrochemical immunosensor, preparation method and application thereof
  • Double-electrode photoelectrochemical immunosensor, preparation method and application thereof

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Effect test

Embodiment 1

[0040] Detection condition optimization

[0041] 1. Fe(NO 3 ) 3 concentration of solution

[0042] Due to Fe-TiO 2 / ZnIn 2 S 4 The photocurrent output of the photoanode has an important influence on the detection sensitivity of the final prepared dual-electrode photoelectrochemical immunosensor, so the following is for Fe-TiO 2 / ZnIn 2 S 4 The preparation process parameters of the photoanode were optimized:

[0043] Because Fe element is in TiO 2 The doping content in nanotubes can be controlled by Fe(NO 3 ) 3 The concentration of Fe(NO 3 ) 3 The concentration is optimized as follows:

[0044] Set the modified area to 0.25cm 2 The Ti foil was vertically inserted into 0.5% HF aqueous solution containing 0mM, 10mM, 30mM, 50mM, 70mM, 90mM Fe(NO 3 ) 3 , using the anodic oxidation method, applying a constant voltage of 10V for 20min, followed by calcination at 450°C in an air atmosphere for 1h, and naturally cooling to room temperature to obtain Fe-TiO 2 Nanotube p...

Embodiment 2

[0065] Fe-TiO 2 Fabrication of nanotube photoanode substrates

[0066] Set the modified area to 0.25cm 2 The Ti foil was vertically inserted into a 0.5% HF aqueous solution containing 50mM Fe(NO 3 ) 3 , using the anodic oxidation method, applying a constant voltage of 10V for 20min, followed by calcination at 450°C in an air atmosphere for 1h, and naturally cooling to room temperature to obtain Fe-TiO 2 Nanotube photoanode substrate.

[0067] Scanning electron microscopy results are attached Figure 6 As can be seen from the figure, Fe-TiO 2 The nanotube photoanode substrate is an array structure composed of a large number of highly ordered nanotubes, with an average diameter of about 50nm and a wall thickness of about 10nm; this structure has a large specific surface area, which is conducive to the subsequent large amount of ZnIn 2 S 4 The deposition of nanocrystals; moreover, the element distribution map in the inset illustrates the TiO 2 Successful doping of Fe elem...

Embodiment 3

[0069] Fe-TiO 2 / ZnIn 2 S 4 Preparation of photoanode

[0070]Fe-TiO prepared by embodiment 2 2 Nanotube photoanode substrates were sequentially immersed in 0.1M Zn(NO 3 ) 2 Methanol solution, 0.1M Na 2 S Methanol / water mixed solution (v / v=1:1), 0.1M InCl 3 Methanol solution, 0.1M Na 2 S methanol / water mixed solution (v / v=1:1) for 2 min each, washed with methanol solution in each step, repeated 3 times, calcined in 180°C air atmosphere for 1 h, and cooled naturally to room temperature.

[0071] Among them, the results of the scanning electron microscope are attached as Figure 7 As shown, it can be seen from the figure that ZnIn 2 S 4 Nanocrystalline particle size is about 10-15nm, uniformly distributed in Fe-TiO 2 The exterior and interior of the nanotube wall. And the Fe-TiO 2 / ZnIn 2 S 4 X-ray diffraction of the photoanode Figure 8 As shown, the results show that ZnIn 2 S 4 The characteristic diffraction peaks at 2θ = 27.8, 30.5° and 47.3° correspond to t...

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Abstract

The invention discloses a double-electrode photoelectrochemical immunosensor, a preparation method and application thereof, and belongs to the technical field of electrochemical biosensors. The sensorcomprises a photo-anode used as a signal conversion element and a biological cathode, and the biological cathode is obtained by modifying the surface of a cathode substrate used as a signal enhancement auxiliary element with a capture antibody recognition probe and dropwise adding an electrode active site confining liquid. Specific immune reaction occurs between the capture antibody recognition probe and a target antigen, and sensitive detection of the target antigen by the double-electrode immunosensor is realized by detecting significant change of an electric signal. The double-electrode photoelectrochemical immunosensor disclosed by the invention not only can effectively improve the detection sensitivity of antigen disease markers, but also has the application potential of precisely detecting a to-be-detected target antigen Ag in an actual complex biological matrix, and is suitable for market popularization and application.

Description

technical field [0001] The invention belongs to the technical field of photoelectrochemical biosensors, and in particular relates to a dual-electrode photoelectrochemical immunosensor jointly constructed by utilizing the synergistic effect of a photoanode and a biocathode, and a preparation method and application thereof. Background technique [0002] With the rapid development of science and technology and the improvement of living standards, people pay more and more attention to their health. Accurate early diagnosis and effective prevention and control of major diseases such as cancer can effectively protect the health of the people, thereby contributing to the promotion of economic and social development. Biosensors are mainly analysis and detection devices composed of signal conversion elements and molecular recognition elements, and are an important way for in vitro diagnosis of common major diseases. Photoelectrochemical biosensor is a new generation of sensing techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N33/53
CPCG01N27/3277G01N33/53
Inventor 范高超钦威穆飞飞
Owner 江苏知至生物科技有限公司
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