Application of MSM in preparation of drugs for destroying cell walls and protecting organs

A cell wall and drug technology, used in antibacterial drugs, drug combinations, and resistance to vector-borne diseases, etc., can solve problems such as unproven and not involving the role of MSM, and achieve the effect of protecting death and damage.

Pending Publication Date: 2021-03-19
中国人民解放军陆军特色医学中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] None of the above related studies involved the effect of MSM on the cell walls of Escherichia coli and drug-resistant bacteria MRSA. Whether MSM can inhibit or kill the above-mentioned bacteria to achieve the purpose of anti-infection through its effect on the cell walls has not yet been confirmed.

Method used

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  • Application of MSM in preparation of drugs for destroying cell walls and protecting organs
  • Application of MSM in preparation of drugs for destroying cell walls and protecting organs
  • Application of MSM in preparation of drugs for destroying cell walls and protecting organs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051]To study the effect of different concentrations of MSM (0, 1ng / ml, 10ng / ml, 100ng / ml, 1ug / ml, 10ug / ml, 100ug / ml, 1mg / ml) on the growth of Escherichia coli, using Escherichia coli in logarithmic growth phase , the initial number of bacteria is 5X10^5CFU, added to 5 ml of LB medium, different concentrations of MSM were added to different centrifuge tubes, 200rpm, 37 degrees Celsius shaking culture, record the OD of the bacterial solution every few hours, and according to the establishment of the research The standard curve is converted to cfu, and the graph is drawn (see attached figure 1 ).

[0052] Conclusion: MSM inhibits the growth of E. coli (Escherichia coli, Gram-negative bacteria) in vitro: It is found that MSM can significantly inhibit the growth of E. coli (E. coli) in logarithmic growth phase. The MIC (Minimuminhibitory concentration, minimum inhibitory concentration) of MSM on the growth of Escherichia coli is 1ng / ml.

Embodiment 2

[0054] To study the effect of different concentrations of MSM (0, 1ng / ml, 10ng / ml, 100ng / ml, 1μg / ml, 10μg / ml, 100μg / ml, 1mg / ml) on the growth of MRSA, the use of MRSA in the logarithmic growth phase The initial bacterial count was 5X10^5CFU, added to 5ml LB medium, different concentrations of MSM were added to different centrifuge tubes, 200rpm, shaking culture at 37 degrees Celsius, and the OD of the bacterial solution was recorded every few hours, and according to the standards established in the study Curve conversion cfu, drawing (see attached figure 2 ).

[0055] Conclusion: In vitro study, MSM inhibits the growth of MRSA (methicillin-resistant Staphylococcus aureus, one of the common drug-resistant bacteria, Gram-positive bacteria) in vitro: it is found that MSM can significantly inhibit the growth of MRSA in the logarithmic growth phase. The MIC (Minimum inhibitory concentration, minimum inhibitory concentration) of MSM on the growth of MRSA is 1ng / ml.

Embodiment 3

[0057] To study whether MSM can destroy the cell wall of Escherichia coli and MRSA bacteria.

[0058] Adjust the bacterial concentration of Escherichia coli and MRSA to 2X10^8 CFU / mL, transfer 200 microliters to a 96-well darkened culture dish, and add different final concentrations of MSM (0, 10 μg / ml, 100 μg / ml, 1000 μg / ml), Incubate at 37°C for 1 hour, add 2.5ul / well of 5g / mL propidium iodide (PI), continue to incubate for 90 minutes, and detect with a fluorescence spectrophotometer (excitation wavelength 535nm, emission wavelength 620nm). According to the results of absorbance value plotted (see attached image 3 , 4 ).

[0059] Conclusion: High concentrations of MSM (100 μg / ml, 1000 μg / ml) can destroy the bacterial walls of Escherichia coli and MRSA.

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Abstract

The invention belongs to the technical field of medicine application, and particularly discloses application of MSM in preparation of drugs for destroying cell walls and protecting organs. The MSM isin contact with escherichia coli or drug-resistant bacteria MRSA, so that the escherichia coli or the drug-resistant bacteria MRSA can be inhibited or killed in the logarithmic phase, and the cell walls are destroyed; the total bilirubin and glutamic oxalacetic transaminase in a lethal dose MRSA acute infection body are remarkably reduced, the obvious protection effect on organs infecting the bodyis achieved, and no obvious difference exists in the protection effect on other organs; after the MSM treatment, expression levels of inflammatory factors IL-6, TNF-alpha and IL-1beta are obviously reduced, and the change levels of other cell factors are not obvious; ROS and NO generated by peritoneal macrophages are reduced, so that less damage is caused to organisms; and the apoptosis and autophagy levels of the peritoneal macrophages are reduced, the time for playing a role in an organism is prolonged, and the effect of preventing acute infection lethal of the lethal dose MRSA is achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine application, and specifically relates to the application of MSM in the preparation of medicines for destroying cell walls and protecting organs. Background technique [0002] The overuse of antibiotics leads to the emergence of superbugs from multi-drug-resistant bacteria. Patients have no available drugs, and the infection continues to aggravate and eventually lead to death. It has become a major health problem that scientists around the world are generally concerned about. It is particularly important to find antibacterial drugs different from antibiotics from nature, and new breakthroughs are expected to be made in the treatment of multi-drug resistant bacterial infections. [0003] Methylsulfonylmethane (MSM, Methylsulfonylmethane), also known as dimethyl sulfone and methyl sulfone, with a molecular weight of 94kd, is a metabolite of dimethyl sulfoxide (DMSO), an organic sulfur-containing com...

Claims

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Application Information

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IPC IPC(8): A61K31/10A61K35/74A61P31/04A61P43/00A61P1/16A61P37/04
CPCA61K31/10A61K35/74A61P31/04A61P43/00A61P1/16A61P37/04Y02A50/30
Inventor 杨霞梁华平马娓何东梅
Owner 中国人民解放军陆军特色医学中心
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