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Frozen microneedle array, preparation method therefor and application of frozen microneedle array

A microneedle array and microneedle technology, applied in the field of biomedical materials, can solve the problems of easy inactivation of biologically active substances, limited range of material selection, and high requirements for the preparation process, and achieve low technical requirements, universality, and simple methods. Effect

Inactive Publication Date: 2021-03-19
NANJING DRUM TOWER HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, the materials that make up the microneedle array need to have a certain mechanical strength to pierce the epidermis, which greatly limits the selection of materials. For example, some materials with high water content cannot be used.
In addition, microneedles can be used to carry and deliver some biologically active substances, but these biologically active substances are often easily inactivated, and have high and different requirements on the environment and preparation process.

Method used

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  • Frozen microneedle array, preparation method therefor and application of frozen microneedle array
  • Frozen microneedle array, preparation method therefor and application of frozen microneedle array
  • Frozen microneedle array, preparation method therefor and application of frozen microneedle array

Examples

Experimental program
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Effect test

Embodiment 1

[0040] A kind of frozen methacrylate gelatin microneedle carrying rhodamine B, its preparation comprises the following steps:

[0041] S1. Prepare drug-loaded microneedle raw material solution A: prepare an aqueous solution containing 15% methacrylate gelatin by mass fraction and 1% 2-hydroxy-2-methylpropiophenone by volume, and mix 1 mg / mL of Rhodamine B;

[0042] S2. Preparation of drug-loaded frozen microneedle array

[0043] S21. Use a pipette gun to drop 100 μl of the drug-loaded microneedle raw material solution A onto the polydimethylsiloxane template 2 with an array of inverted conical holes (the hole spacing is 200 μm, the hole depth is 300 μm, and the hole diameter is 150 μm). Surface, vacuum treatment for 5 minutes to make it fully filled in the inverted conical hole, use a pipette gun to suck away the excess microneedle raw material solution 1 outside the inverted conical hole, and irradiate with ultraviolet light for 20s to make the drug-loaded drug in the inver...

Embodiment 2

[0047] A frozen calcium alginate microneedle carrying green fluorescently labeled insulin, the preparation of which comprises the following steps:

[0048] S1. Prepare drug-loaded microneedle raw material solution B: prepare a very low-viscosity sodium alginate solution with a mass fraction of 4%, and mix green fluorescently labeled insulin (final content 0.5 mg / mL) into it;

[0049] S2. Preparation of drug-loaded frozen microneedle array

[0050] S21. Use a pipette gun to drop 400 μl of the drug-loaded microneedle raw material solution B onto the polydimethylsiloxane template 2 with an array of inverted conical holes (the hole spacing is 700 μm, the hole depth is 850 μm, and the hole diameter is 600 μm). Surface, centrifuged at 1000rpm for 3min to make it fully filled in the inverted conical hole, use a pipette gun to suck away the excess drug-loaded microneedle material solution B outside the inverted conical hole, and drop 400μl mass fraction of 10 to the surface of the hol...

Embodiment 3

[0054] A kind of frozen Matrigel microneedle carrying Bacillus subtilis, its preparation comprises the steps:

[0055] S1. Prepare the microneedle raw material solution C carrying living microorganisms: mix Bacillus subtilis (purchased from Beina Biological Co., Ltd.) with Matrigel (Corning, USA), and the final content of Bacillus subtilis is 10 8 / mL;

[0056] S2. Preparation of drug-loaded frozen microneedle array

[0057] S21. Use a pipette gun to drop 300 μl of the drug-loaded microneedle raw material solution C onto the polydimethylsiloxane template with an array of inverted pyramidal holes (the hole spacing is 400 μm, the hole depth is 800 μm, and the hole diameter is 600 μm). 2 surface, centrifuge at 1000rpm for 3min to make it fully filled in the inverted pyramid-shaped hole, use a pipette gun to suck away the excess drug-loaded microneedle material solution C outside the inverted conical hole, and place it at 37°C for 20min , the drug-loaded microneedle raw material...

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Abstract

The invention discloses a frozen microneedle array, a preparation method therefor and application of the frozen microneedle array. The frozen microneedle array is prepared by a method of introducing afreezing process during template replication on the basis of a principle that aqueous substances can become hard from soft by freezing. The preparation method is simple in operation, facilitates popularization and can be applied to large-scale production, the prepared frozen microneedle array can be prepared from various solutions or aquagel, restrictions of microneedles to material selecting arebroken away; and moreover, the freezing process scarcely damages activity of bioactive substances, so that frozen microneedles have capacity for carrying a variety of bioactive substances, have better universality and are advantageously applied to a variety of biological and medical fields.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a frozen microneedle array and its preparation method and application. Background technique [0002] The microneedle array is mainly composed of a substrate and micron-sized needle tips, which can penetrate the epidermis without contacting capillaries and nerve endings, so it can significantly enhance skin permeability and drug absorption rate, providing a microneedle Invasive, painless transdermal drug delivery. In recent years, the field of microneedling has developed rapidly, but there are still some challenges. First of all, the materials that make up the microneedle arrays need to have certain mechanical strength to pierce the epidermis, which greatly limits the range of material choices, for example, some materials with high water content cannot be used. In addition, microneedles can be used to carry and deliver some biologically active substances, but these ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61M37/00
CPCA61M37/0015A61M2037/0053A61M2037/0023A61M2037/0046
Inventor 赵远锦张筱萱王月桐张大淦商珞然
Owner NANJING DRUM TOWER HOSPITAL
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