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Extraction method for antibacterial peptide

An extraction method and antimicrobial peptide technology, which is applied in the field of extraction of chemical raw materials, can solve the problems of antimicrobial peptides with many impurities, expensive antibacterial costs, and complicated processes, and achieve the effects of less impurities, sedative effect, and simple operation

Pending Publication Date: 2021-03-19
广东鑫皇冠新材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, in order to solve the problem that obtaining a large amount of antimicrobial peptides in the prior art is limited by the technical level of polypeptide synthesis, the antibacterial cost obtained by chemical synthesis is too expensive, and the extraction of antimicrobial peptides from animals and plants generally has complicated processes and cumbersome steps, and the extraction rate Low, the extracted antimicrobial peptide has too many impurity components, resulting in low expression rate and poor effect. The invention provides a method for extracting antimicrobial peptide. The specific technical scheme is as follows:

Method used

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  • Extraction method for antibacterial peptide
  • Extraction method for antibacterial peptide

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Experimental program
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Effect test

Embodiment 1

[0076]A method of extraction of antimicrobial peptides, the extraction method comprising the steps of:

[0077]According to the weight of the barley, purple alfalfa, pea, and sesame mixture, the total weight of plant raw materials is 8kg, including 1kg, peas, 2kg, sesame, and 4kg of peas, 2kg, 2kg of peas, 2kg of peas, 4kg, 2kg, 2kg, 2kg of peas, 2kg, 2kg of peas, 2kg, 2kg, 2kg of peas

[0078]The plant raw material was added to the treatment kettle, and a concentration of 8% acetic acid was added to the treatment kettle according to the ratio of the material weight ratio of 1: 15;

[0079]By the online control system, the temperature condition in the treatment kettle is 40 ° C; the pressure condition is 20 MPa; the power of the ultrasonic treatment conditions is 150W, the frequency is 45 kHz, the time is 20 min, and the first phase is processed, and the aperture is used. 15 nm ceramic film filtration to give the filtrate A;

[0080]0.01 g / ml of 0.01 g / ml (amount of the addition amount of t...

Embodiment 2

[0084]A method of extraction of antimicrobial peptides, the extraction method comprising the steps of:

[0085]According to the weight of the barley, the purple alfalfa, pea and sesame seeds are mixed with plant raw materials, and the total weight of plant raw materials is 14kg, of which 1kg of barley, 5kg, peas, 7kg, sesame seeds;

[0086]The plant raw material was added to the treatment kettle, and a concentration of 0.5% acetic acid was added to the treatment kettle according to the ratio of the material weight ratio of 1:30;

[0087]By the online control system, the temperature condition in the treatment kettle is 85 ° C; the pressure condition is 40 MPa; the power of the ultrasonic treatment conditions is 300W, the frequency is 45 kHz, the time is 45min, and the first phase is processed, the aperture is used. 35 nm ceramic film filtration to give the filtrate A;

[0088]0.09 g / ml of 0.09 g / ml (amount of filtrate A) was added to a papaino, cellulase, and mannanase mixed to obtain a comp...

Embodiment 3

[0092]A method of extraction of antimicrobial peptides, the extraction method comprising the steps of:

[0093]According to the weight of the barley, purple alfalfa, pea and sesame mixture with plant raw materials, total weight of plant raw materials, 1kg, 3kg, peas, 3kg, 3kg, peas;

[0094]The plant feedstock was added to the treatment kettle, and a concentration of 5% acetic acid was added to the treatment kettle according to the ratio of the material weight ratio of 1:20;

[0095]By the online control system, the temperature condition in the treatment kettle is 50 ° C; the pressure condition is 30 MPa; the power of the ultrasonic treatment conditions is 250W, the frequency is 40 kHz, the time is 35min, and the first phase is processed, the aperture is used. 25 nm of ceramic film filtration to give the filtrate A;

[0096]0.08 g / ml of the filtrate A (amount) of 0.08 g / ml of the filtrate A is added to a papaino, a cellulase, and mannanase mixed to obtain a composite enzyme, an enzymatic co...

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Abstract

The invention provides an extraction method for an antibacterial peptide. The antibacterial peptide is obtained through mixing a variety of botanical raw materials and carrying out extracting. The obtained antibacterial peptide has spectral applicability, the molecular weight of overall active regions is smaller than 5kDa, and the antibacterial peptide has a stable disulfide bond structure and isgood in acid resistance, organic solvent resistance and thermal stability; the extraction method is simple in operation, the production efficiency is increased, the production cost is reduced, and thus, the extraction method is in line with industrial production; and the extracted antibacterial peptide has a remarkable inhibiting action on propionibacterium acnes, can be effectively applied to anti-acne products and exerts excellent bacteriostatic, inflammation diminishing and calming effects. Furthermore, the extraction method employs an online control system, treatment conditions of a treatment still can be more accurately controlled, the impurity ingredients are few, and thus, the quality of extracting is guaranteed.

Description

Technical field[0001]The present invention relates to the field of extraction of chemical raw materials, in particular, to an extraction method of antimicrobial peptides.Background technique[0002]Antibacterial peptide is a kind of defensive polypeptide having broad-spectrum antibacterial, due to the composition of the antimicrobial peptide. Structure and functional differences, there is a different antibacterial mechanism, and the antimony peptide is largely affected by the function of antimicrobial peptide, thereby determining a variety of functions of antibacterial peptide, existing Technically antimicrobial peptides are generally used to antibiotic drug resistance bacteria, showing the body to the body to promote tissue healing and regulating the body's immune system, and its application is extremely small in the surface of the body, and therefore also has a wide range of application space. However, in the prior art, a large number of antimicrobial peptides are obtained by the te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/20C12P21/06A61K38/01A61P17/10A61P31/04A61K8/64A61Q19/00A61Q17/00
CPCC12P21/06C07K1/36C07K1/34C07K1/20A61K38/011A61P31/04A61P17/10A61K8/64A61Q19/00A61Q17/005A61K2800/84A61K2800/82
Inventor 谭贺洵张明远胡海宏
Owner 广东鑫皇冠新材料有限公司
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