Colorectal cancer excrement protein biomarker as well as kit and application thereof

Abstract

The invention relates to the field of immunodetection and particularly relates to a colorectal cancer excrement protein biomarker and a kit and application thereof. The biomarker comprises at least atransferrin receptor. It is found for the first time that the transferrin receptor can be used for fecal screening of colorectal cancer, the colorectal cancer screening sensitivity and the negative and positive coincidence rate can be remarkably improved by means of the biomarker containing the transferrin receptor, rapid and convenient colorectal cancer preliminary screening is achieved, the colorectal mirror efficiency is improved, and colorectal cancer screening cost is reduced; the price can be received by the public, the detection time is short, and the method is suitable for POCT and household detection and has wide application value.

Description

technical field [0001] The invention relates to the field of immunodetection, in particular to a stool protein biomarker for colorectal cancer, a kit and application thereof. Background technique [0002] Colorectal cancer (colorectal cancer, CRC) is a tumor with high morbidity and mortality worldwide. In addition to genetic factors, the main pathogenic factors are food and intestinal microorganisms. With the improvement of living standards, the proportion of high-fat, high-protein, and low-fiber foods in the dietary structure of Chinese residents has increased due to various reasons. The incidence and mortality of CRC in China have increased steadily year by year, especially among urban white-collar workers. Beijing, Shanghai, etc. The city has firmly occupied the top three. However, the morbidity and mortality in the United States have decreased significantly. In 1976, the incidence rate of colorectal cancer in the United States was 60.5 per 100,000, and it dropped to 46...

AI Technical Summary

Problems solved by technology

Occult blood screening (now mainly immunological method, chemical method is gradually fading out of the market due to poor specificity) is more convenient, but the effect is not ideal: many medical examination packages include occult blood items, due to unstable reagent quality and other reasons, the actual sensitivity Often it can only reach 3-30%, and the rate of abandonment is high. General screening has time requirements for sending samples to testing points (hospital centers, clinics), and requires delivery within 2-4 hours (practical operations often fail to meet the requirements) This requirement); in addition to the strong smell, the medical examiners are unwilling to do it, and the doctors are unwilling to promote it, so the promotion response has not been high for many years

Some companies are now developing reagents based on nucleic acid detection (DNA mutation and / or methylation, microRNA expression), as well as methods such as liquid biopsy and intestinal flora NGS that are still in the clinical verification stage. The accuracy of these methods has been greatly improved, but The price is expensive and the detection cycle is long. It is not the most popular screening method for the majority of urban and rural residents.

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