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Test paper box for detecting anticancer drugs in body fluids and its preparation and use method

A technology of anticancer drugs and test strips, which is applied in measuring devices, material stimulation analysis, instruments, etc., can solve the problems of restricting the development of real-time drug testing equipment, hindering the clinical application of drug testing, and bulky testing instruments, so as to promote standardization and integration, improved pre-concentration performance, and high sensitivity

Active Publication Date: 2022-04-05
XIDIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, due to the complex composition of biological fluids, the detection of specific substances in biological fluids usually requires some complicated pretreatment processes, such as purification, preconcentration, etc., which involve various pretreatment instruments such as centrifuges and chromatographic columns; on the other hand , detection instruments are often bulky and require expensive reagents, time-consuming sample preparation, and operator expertise, which in this case not only hinders the clinical application of drug testing, but also restricts the development of point-of-care drug testing devices, Therefore, there is an urgent need to develop a detection device that is small in size, compact in structure, easy to carry, simple to make, low in cost, and easy to operate.

Method used

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  • Test paper box for detecting anticancer drugs in body fluids and its preparation and use method
  • Test paper box for detecting anticancer drugs in body fluids and its preparation and use method
  • Test paper box for detecting anticancer drugs in body fluids and its preparation and use method

Examples

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Embodiment 1

[0080] This embodiment provides a test kit for the detection of anticancer drugs in body fluids, such as Figure 1a-1b As shown, it includes a test paper and a detection box; the test paper includes a paper tip and a tail core connected to the paper tip, and the paper tip deposits metal nanomaterials as a SERS substrate to enhance the signal of Raman scattering, and can realize the detection of drugs in biological fluids. pre-concentration (refer to Figure 2a ), the above-mentioned metal nanomaterial can be gold or silver, and its shape is nanowire, nanorod, nanoparticle (nanosphere), nanostar, nanosheet etc.; Test paper box comprises dustproof cover (reference Figure 2b ), injector (reference Figure 2c ) and a box body, the box body is provided with a slope for placing test paper and a cavity for placing a sample injector, the cavity communicates with the slope, the slope of the slope is an acute angle, and a waste liquid pool is also provided under the slope for collecti...

Embodiment 2

[0090] In order to make the beneficial effects of the present invention more obvious, four sets of comparative experiments were done in this embodiment to select the optimal solution.

[0091] Experiment 1: On the basis of embodiment 1, the length of the paper point is 40mm, the initial width is 6.6mm, the length of the tail core is 10mm, and the angle of the paper point is 10° (such as Figure 2a ) test paper, and simultaneously prepare test papers with paper point angles of 15°, 20°, 25°, and 30° to carry out experiments respectively; , the sample solution gathers at the tip of the paper under the action of capillary action and the component of gravity along the slope direction, adding the vector of the gravity component drives more solution to the tip of the paper, absorbs more samples, and improves the preconcentration. In addition, The residual solution directly and quickly enters the waste liquid pool, which shortens the drying time in the later stage; in order to quanti...

Embodiment 3

[0114] The detection of anticancer drugs in biological fluids is of great significance to clinical chemotherapy, but the existing detection technology is still stuck in time-consuming laboratory research, epirubicin hydrochloride and cyclophosphamide are anticancer drugs widely used in clinical , in this embodiment, the combination of the test kit for the detection of anticancer drugs in body fluids of the present invention and its preparation and use methods is used to quickly and directly quantitatively determine the hydrochloride hydrochloride ratio in bovine serum and artificial urine Star, and cyclophosphamide in bovine serum and the mixture of epirubicin hydrochloride and cyclophosphamide, further prove the beneficial effect of the present invention.

[0115]In this example, on the basis of Example 1 and Example 2, without other pretreatments, the test paper box of the present invention and its method of use are used for direct preconcentration to detect epirubicin hydroc...

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Abstract

The invention belongs to the technical field of drug detection, and specifically discloses a test paper box for detecting anticancer drugs in body fluids and a preparation and use method thereof. The invention provides a test paper box including a test paper and a detection box. The test paper includes a paper tip and a tail core connected to the paper tip. Metal nanomaterials are deposited on the paper tip as a SERS substrate to enhance the signal of Raman scattering. The test paper box includes The sampler and the box body, the box body is provided with a slope for placing the test paper and a cavity for placing the sample injector, the slope is connected, the tip of the test paper is placed on the slope, and the tail core is just placed on the sample injector below; the present invention is simple to manufacture, low in cost, does not need bulky equipment and aseptic conditions, and the produced test paper box is small in size, compact in structure, easy to carry, and the time-consuming of the test paper box is short during use, The operation is convenient and simple, and the standardization and integration of SERS detection are promoted. The method can also significantly improve the pre-concentration effect of the drug, and the detection sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of drug detection, and in particular relates to a test paper box for detecting anticancer drugs in body fluids and a preparation and use method thereof. Background technique [0002] Drug detection in biological fluids has always been an important part of clinical diagnosis. At present, there are many detection techniques, such as high performance liquid chromatography, immunoassay, liquid chromatography-mass spectrometry (LC-MS), etc., which can detect biological fluids. Due to their multi-instrument collaboration and multi-step operation, it not only increases the risk of introducing human error, but also limits their application to the laboratory. On the one hand, due to the complex composition of biological fluids, the detection of specific substances in biological fluids usually requires some complicated pretreatment processes, such as purification, preconcentration, etc., which involve various pretreat...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 胡波宜浪浪武建铎程贺
Owner XIDIAN UNIV