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Flammulina filiformis strain and identification and breeding method thereof

An identification method, the technology of Flammulina velutipes, applied in the field of edible fungi, can solve the problems of fast and accurate identification of mononuclear, dinuclear and hybrid strains of Flammulina velutipes, and achieve the effects of not easy browning, easy operation, and high biological efficiency

Active Publication Date: 2021-03-26
河北省农林科学院经济作物研究所
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Problems solved by technology

[0004] Therefore, the technical problem to be solved in the present invention is to overcome defects such as the mononucleus, dinucleus and hybrid strains of Flammulina velutipes that can not be identified quickly and accurately in the prior art, thereby providing a kind of Flammulina velutipes bacterial strain and its identification and breeding method

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  • Flammulina filiformis strain and identification and breeding method thereof
  • Flammulina filiformis strain and identification and breeding method thereof
  • Flammulina filiformis strain and identification and breeding method thereof

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Embodiment 1

[0050] A strain of Flammulina velutipes, preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, address: Institute of Microbiology, Chinese Academy of Sciences, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCCNo.19644, and the preservation date is May 2020 On March 26, it was named Jijin 12; the classification was named: Flammulina filiformis.

[0051] An identification method of Flammulina velutipes strains, one of the two nuclei of the double monohybrid is that the acceptor nucleus is derived from the nucleus of the mononuclear parent, and the other is that the donor nucleus is derived from one of the nuclei of the dinuclear parent. The parents of Jijin 12 include the monokaryotic strain parent and the dikaryotic strain parent. The monokaryotic strain parent is obtained after protoplast monokaryon preparation and regeneration of Flammulina velutipes strain HUANG1, and the dikar...

Embodiment 2

[0073] The breeding method of above-mentioned Flammulina velutipes bacterial strain, comprises the steps:

[0074] Inoculate the mycelium of Flammulina velutipes strain HUANG1 in an Erlenmeyer flask filled with liquid medium, culture it statically at 25°C for 8 days, and shake the flask by hand every 24 hours. Mycelia, with a mass concentration of 2% lysozyme enzyme solution (purchased from Guangdong Provincial Institute of Microbiology), enzymolysis at 30 ° C for 4 hours, filtered off broken mycelia, washed 3 times with 0.6M sucrose solution, and purified Dilute the protoplast suspension with 0.6M sucrose solution to a concentration of 300 cells / ml, draw 0.2ml and spread it on the regeneration medium, cultivate it at 25°C for 8 days, pick a single colony and store it on the slant of the test tube, and store it on the slant of the test tube Cultivate at 25°C for 4 days, take mycelium slices, and observe under a microscope whether there is a lock junction. If there is no lock j...

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Abstract

The invention belongs to the technical field of edible mushrooms, and particularly relates to a flammulina filiformis strain identification and breeding method. The strain breeding method includes thefollowing steps: carrying out di-mon hybridization by taking protoplast mononuclear strains of cultivated strains as mononuclear parents and wild strains as binuclear parents, identifying the authenticity of hybrids, and further screening hybrids in the hypha stage and fruiting stage to obtain excellent hybrids, and finally, carrying out single-spore selfing, tissue isolation and fruiting screening on the hybrids obtained by screening to obtain excellent flammulina filiformis strains. The identification method of the hybrid strains includes the steps of carrying out mycelium culture on the flammulina filiformis parent strains and the protoplast mononuclear strains thereof, and then performing DNA extraction, internal transcribed spacer (ITS) sequence amplification and sequencing, single nucleotide polymorphism (SNP) locus analysis, specific primer design and screening, and carrying out AS-PCR amplification and electrophoresis detection on the parent strains and the hybrid strains. Thestrain has a golden yellow pileus, a bright color and a fine stipe, the stipe is yellowish white and is not prone to brown, and the basal part of the stipe has few fluffs and is not sticky.

Description

technical field [0001] The invention belongs to the technical field of edible fungi, and in particular relates to a Flammulina velutipes strain and identification and breeding methods thereof. Background technique [0002] Flammulina filiformis belongs to the genus Shiitake, and its Chinese name is silk cover winter mushroom. It has been using the Latin scientific name of F. velutiper (Fr) Sing until 2018, when Yang Zhuliang and his team from the Kunming Institute of Botany, Chinese Academy of Sciences conducted a research on Chinese Flammulina velutiper To rectify the name, studies have proved that Flammulina velutipes in my country is different from F. velutiper in Europe and the United States. It is a new species, and the Latin scientific name of F. filiformis should be adopted. Flammulina velutipes is rich in various nutrients, because it is rich in "lysine", it is also called "education mushroom". Flammulina velutipes is the fastest growing edible fungus species in my c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N15/04C12Q1/6858C12Q1/6895C12Q1/04A01G18/00C12R1/645
CPCC12N15/04C12Q1/6858C12Q1/6895A01G18/00C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 李慧高春燕王朝江夏伟伟董庆武
Owner 河北省农林科学院经济作物研究所
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