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Application of cinnabar microbial leaching solution in preparation of medicine for treating diabetic foot

A technology of microbial leaching and diabetic foot, which is applied in the field of medicine, can solve problems such as the inability to accurately know the antibacterial effect and which pathogenic bacteria to inhibit, the mercury content and toxicity, the inability to carry out stable quality control, and the complex components of traditional Chinese medicine compositions. The effect of obvious antibacterial effect, safe medication, and simple ingredients

Inactive Publication Date: 2021-04-02
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because cinnabar is often used as undissolved rough mineral powder in medicine, resulting in large dosage and serious toxicity exceeding the standard, there are often people who use cinnabar to cause adverse reactions
Therefore, cinnabar and Chinese patent medicines containing cinnabar are restricted in the international market
Chinese invention patents (CN103656091B and CN102258655B) both disclose pharmaceutical compositions containing cinnabar for the treatment of diabetic foot, but the cinnabar is still used as raw mineral powder, and the mercury content and toxicity in the cinnabar cannot be controlled stably. The ingredients are complex, and it is impossible to know exactly whether each Chinese medicine has an antibacterial effect and what kind of pathogenic bacteria it inhibits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 Preparation of cinnabar microbial leachate.

[0024] Weigh 1.0 g of cinnabar powder with a particle size not greater than 74 μm, add 90 mL of sterilized 9K iron-free liquid medium to a sterilized 250 mL Erlenmeyer flask, and inoculate Thiobacillus ferrooxidans with a volume fraction of 10% ( Thiobacillus ferrooxidans ) and 1% FeS0 4 ·7H 2 0, adjust the pH to 1.8 with sulfuric acid; after weighing the mass, shake the flask for leaching for 25 days at 30°C and an oscillation speed of 120r / min; during the experiment, use sulfuric acid with a volume ratio of 1:1 to adjust the pH value, and use The evaporated water is replenished with distilled water, and the ore liquid at the end of leaching is suction-filtered, and the ore liquid is filtered and sterilized by a bacterial filter. The resulting soluble mercury measured by inductively coupled plasma emission spectrometry (ICP-AES) was 0.282 μg / ml.

Embodiment 2

[0025] Example two Bacteriostatic effect evaluation.

[0026] Activated bacteria solution (fungus collects spores, cultures spore suspension) is diluted to 10 with sterile physiological saline 5 -10 6 cfu / ml spare. Add 0.2ml of bacterial solution to dry NA (bacteria) and PDA (fungi) medium plates, and spread evenly. Leave it for 5 minutes to allow the medium to fully absorb the bacterial solution. Place 4 sterilized Oxford cups on each plate for 5 minutes, so that the Oxford cups are tightly adsorbed on the surface of the medium. Add the cinnabar microbial leaching solution and the same concentration of HgCl to the Oxford cup 2 solution, were added 100μl. Then place the plate in a constant temperature incubator at 35°C for 24-48 h, take it out, measure the diameter of the inhibition zone with a caliper, calculate the average value, and use the diameter of the inhibition zone D to represent the antibacterial activity. The antibacterial effect judgment standard is: D=8mm ...

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Abstract

The invention belongs to the field of medicines, and particularly relates to application of a cinnabar microbial leaching solution in preparation of a medicine for treating diabetic foot. The cinnabarmicrobial leaching solution is found to have broad-spectrum antibacterial and bactericidal effects. Common infected bacteria of the diabetic foot comprise gram-positive bacteria including staphylococcus aureus and drug-resistant staphylococcus epidermidis, gram-negative bacteria including escherichia coli, enterococcus faecalis and pseudomonas aeruginosa as well as deep infected fungi including candida albicans and the like, which are sensitive to the cinnabar microbial leaching solution. Even if the cinnabar microbial leaching solution is diluted by 2 times or 4 times, the cinnabar microbialleaching solution still has good antibacterial and bactericidal effects. The result shows that the cinnabar microbial leaching solution can treat diabetic foot infection and can be used for preparingthe medicine for treating the diabetic foot.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of cinnabar microbial leachate in the preparation of medicine for treating diabetic foot. Background technique [0002] According to the latest global diabetes map (IDF Diabetes Atlas, 2019 edition) released by the International Diabetes Federation (IDF), in 2019, about 463 million adults aged 20-79 suffered from diabetes worldwide. According to its forecast, by 2030, adult diabetes Patients will reach 578 million and 700 million in 2045. Diabetic foot (DF) occurs in about 15% of diabetic patients (Song Wei et al., 2019). Diabetic foot, as one of the common complications of diabetes, is more likely to occur in patients with a diabetes course of more than 10 years, and about 40% of patients with a course of more than 10 years have diabetic feet (Chen Chunjun et al., 2020). [0003] Diabetic foot refers to foot infection, ulceration, and / or deep tissue destruct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/28A61P17/02A61P3/10A61P31/04A61P31/10
CPCA61K33/28A61K9/0014A61P17/02A61P3/10A61P31/04A61P31/10Y02A50/30
Inventor 李红玉付献瑶葛广飞李洋
Owner LANZHOU UNIVERSITY