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Biodegradation method of lignin

A biodegradation and lignin technology, applied in the use of microorganisms/enzymatic cellulose treatment, papermaking, textiles and papermaking, etc., can solve the problems of few researches on bacteria, and achieve the effect of easy operation, good degradation effect and good stability

Inactive Publication Date: 2021-04-02
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Nowadays, the eyes of highly efficient biodegrading bacteria are all on fungi, so most of the research now focuses on how fungi can efficiently degrade lignin, and there is indeed little research on bacteria

Method used

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  • Biodegradation method of lignin
  • Biodegradation method of lignin
  • Biodegradation method of lignin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] The biodegradation operation steps of a kind of lignin are as follows:

[0082] (1) Cultured strains

[0083] Raoultia ornithini Raoultella ornithinolytica Inoculate one loop of slant strains on LB solid medium, culture at 28°C for 24-72 hours to obtain activated strains;

[0084] (2) Preparation of bacterial suspension

[0085] Inoculate the activated strain on LB liquid medium with an inoculum size of 2%, and cultivate it on a shaker at a temperature of 28°C and 180r / min for 2 to 3 days to obtain a fermentation broth;

[0086] Take 2ml of fermentation broth and inoculate it into 100ml of LB liquid medium, culture on a shaker at 28°C and 180r / min for 2-3 days to obtain a bacterial suspension;

[0087] (3) Processing lignin materials

[0088] The lignin material in this embodiment is corn stalks;

[0089] drying the corn stalks at a temperature of 45°C, crushing them; sieving through a 30-40 mesh sieve, and drying at a temperature of 45°C again to obtain corn stalk...

Embodiment 2

[0094] The biodegradation operation steps of a kind of lignin are as follows:

[0095] (1) Cultured strains

[0096] Raoultia ornithini Raoultella ornithinolytica Inoculate one loop of slant strains on LB solid medium, culture at 28°C for 24-72 hours to obtain activated strains;

[0097] (2) Preparation of bacterial suspension

[0098] Inoculate the activated strain on LB liquid medium with an inoculum size of 2%, and cultivate it on a shaker at a temperature of 28°C and 180r / min for 2 to 3 days to obtain a fermentation broth;

[0099] Take 2ml of fermentation broth and inoculate it into 100ml of LB liquid medium, culture on a shaker at 28°C and 180r / min for 2-3 days to obtain a bacterial suspension;

[0100] (3) Processing lignin materials

[0101] The lignin material of this embodiment is wheat straw;

[0102] Drying the wheat straw at a temperature of 45°C and pulverizing it; sieving through a 30-40 mesh sieve, and drying at a temperature of 45°C again to obtain wheat ...

Embodiment 3

[0107] The biodegradation operation steps of a kind of lignin are as follows:

[0108] (1) Cultured strains

[0109] Raoultia ornithini Raoultella ornithinolytica Inoculate one loop of slant strains on LB solid medium, culture at 28°C for 24-72 hours to obtain activated strains;

[0110] (2) Preparation of bacterial suspension

[0111] Inoculate the activated strain on LB liquid medium with an inoculum size of 2%, and cultivate it on a shaker at a temperature of 28°C and 180r / min for 2 to 3 days to obtain a fermentation broth;

[0112] Take 2ml of fermentation broth and inoculate it into 100ml of LB liquid medium, culture on a shaker at 28°C and 180r / min for 2-3 days to obtain a bacterial suspension;

[0113] (3) Processing lignin material

[0114] The lignin material of the present embodiment is apple branch;

[0115] Drying the apple branches at a temperature of 45°C, crushing them; sieving through a 30-40 mesh sieve, and drying at a temperature of 45°C again to obtain ...

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Abstract

The invention discloses a biodegradation method of lignin, and belongs to the technical field of lignin degradation. The method comprises the following operation steps: (1) inoculating a Raoultella ornithinolytica slant strain for dissolving ornithine into an LB solid culture medium so as to obtain an activated strain; (2) inoculating the activated strain into an LB liquid culture medium to obtainfermentation liquor; fermenting the fermentation broth again to obtain a bacterial suspension; (3) drying and crushing the treated lignin; (4) adding the powder into a liquid inorganic salt culture medium for fermentation, adding a fermentation product into the bacterial suspension, and stirring for reaction to obtain a degradation product, wherein the degradation rate of lignin reaches 20%-25%,and the surface of the degradation product is in a loose and porous structure state. Compared with an existing method, the Raoultella ornithinolytica strain for dissolving ornithine has the advantagesthat the adaptability of the Raoultella ornithinolytica strain to the growth environment of bacteria is high, and peroxidase, manganese peroxidase and laccase generated by the bacteria have the excellent characteristics of being better in stability, wider in optimal pH range, more tolerant to chloride ions and the like. The method is mild in reaction and easy to operate.

Description

Technical field [0001] The invention belongs to the technical field of lignin degradation, and specifically relates to a biodegradation method of lignin. Background technique [0002] Lignocellulose is a biomass composed of lignin, cellulose and hemicellulose. It is a difficult-to-degrade cell wall component of woody and herbaceous plants. Cellulose serves as the skeleton, and lignin and cellulose are dispersed in the fiber in an inclusive form. In or around the elements, the combination of the three is very stable and is the main raw material for current biomass research. However, due to its compact structure, it is not easy to degrade and is greatly hindered in production and utilization. Currently, commonly used lignin treatment methods mainly include physical methods, chemical methods, biological methods, or a combination of several methods. Although these methods increase the utilization of biomass resources to a certain extent, these methods still have many disadvant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D21C5/00
CPCD21C5/005
Inventor 刘国庆韩东晶杨少华吕欣吴建鑫孙苗苗王志花周宁
Owner HEFEI UNIV OF TECH
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