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A strain of Serratia marcescens and its application

A technology of Serratia marcescens and culture medium, which is applied in the field of microbial restoration of inorganic pollutants, can solve the problems of serious secondary pollution, high cost, long time consumption, etc., and achieves the effect of improving the content of available phosphorus in soil

Active Publication Date: 2022-03-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the physical and chemical methods are limited due to the disadvantages of large amount of engineering, high cost, long time consumption and serious secondary pollution.

Method used

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  • A strain of Serratia marcescens and its application
  • A strain of Serratia marcescens and its application
  • A strain of Serratia marcescens and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Screening, isolation and purification and identification of embodiment 1 bacterial strain C273

[0052] 1. Screening method of a cadmium-resistant and cadmium ion-removing bacterial strain C273

[0053] 1. Material preparation

[0054] Soil source for bacterial strain screening: The rhizosphere soil of paddy fields around Dabaoshan, Shaoguan City, Guangdong Province was collected. The soil samples were sealed with sampling bags, and brought back to the laboratory at 4°C for storage for later use.

[0055] LB medium: peptone 10.0g, yeast extract powder 5.0g, NaCl 10.0g, pH7.0-7.2, distilled water to 1L; solid medium plus 18.0g agar powder. Sterilize at 121°C for 15 minutes.

[0056] Inorganic salt medium (MSM medium): 5mL phosphate buffer solution (KH 2 PO 4 8.5g·L -1 、K 2 HPO 4 ·H 2 O2 1.75g·L -1 、Na 2 HPO 4 12H 2 O 33.4g·L -1 , NH 4 Cl 5.0g L -1 ), 3.0mL 22.5g·L -1 MgSO 4 Solution (MgSO 4 ·7H 2 O 46.125g·L -1 ), 1.0mL 0.25g·L -1 FeCl 3 Solution ...

Embodiment 2

[0079] Example 2 bacterial strain C273 contains Cd 2+ Cd in inorganic salt medium with peptone 2+ removal of

[0080] Streak the strain C273 on the LB plate and culture it at 30°C for 20 hours, pick a single colony and inoculate it in the LB liquid medium, culture it in a shaker at 30°C and 150 rpm for 20 hours, and then inoculate it at a ratio of 2% (v / v) to Cd 2+ Concentrations of 10, 20, 40 and 80mg·L -1 containing 10g·L -1 Tryptone was cultured in MSM medium, and samples were taken after 6 d (days); no addition of strain S1 was used as the control (CK), and three repeated experiments were set up. The sample liquid was centrifuged at 80,000 rpm for 10 min, and the supernatant was filtered with a 0.22 μm filter membrane, and the Cd was measured by flame atomic absorption spectrometry (AAS) after filtration. 2+ Residual amount (method is with embodiment 1).

[0081] Cd after 6 days of culture 2+ removal efficiency as image 3 Shown: The results show that after the add...

Embodiment 3

[0082] Phosphorus-dissolving and potassium-dissolving qualitative test of embodiment 3 bacterial strain C273

[0083] Phosphorus and potassium solubilization of bacterial strain C273 (phosphate / potassium solubilizing bacteria, meaning to convert insoluble phosphorus and potassium into soluble phosphorus and potassium) (specific references: Rawat, P., Das, S., Shankhdhar, D., et al., Phosphate-solubilizing microorganisms: Mechanism and their role in phosphate solubilization and uptake [J]. Journal of Soil Science and Plant Nutrition. 2020, https: / / doi.org / 10.1007 / s42729-020-00342-7.) Functional qualitative test: Inoculate the strain C273 drawn on LB solid medium into LB liquid medium, shake and culture at 125~150rpm, 28~30℃ for 18~24h, then Spot on PKO culture medium and potassium bacteria solid culture medium respectively, and inoculate 2% on PKO liquid culture medium and potassium bacteria liquid culture medium, establish 3 repeated experiments, place 30 ℃ incubator or shake...

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Abstract

The invention discloses a strain of Serratia marcescens and its application. The name of the strain is Serratia marcescens subsp.sakuensis C273, and the preservation number is GDMCC NO: 61042. The strain was preserved in Building 59, Compound 59, No. 100 Xianlie Middle Road, Guangzhou City on June 3, 2020. The Guangdong Provincial Microbial Culture Collection Center. The present invention finds through experiments that the Serratia marcescens can remove cadmium ions in soil and water through co-metabolism, and can also reduce the pH in the system so as to dissolve insoluble phosphorus and potassium substances, illustrating that in the present invention Serratia marcescens has the potential to remove cadmium ions, increase soil available phosphorus and potassium content, and can be used for environmental pollution control and plant growth promotion.

Description

technical field [0001] The invention relates to the technical field of microbial remediation of inorganic pollutants, in particular to a strain of Serratia marcescens and its application. Background technique [0002] As one of the heavy metals, cadmium (Cadmium, Cd) is not an essential element for the growth and development of the body. After being absorbed and enriched by animals, plants and the human body, it has serious toxic effects. Under the original natural conditions, the environmental concentration of cadmium is lower than the toxic concentration of animals and plants, the distribution range is limited, and it is in a state of cyclic balance, and has not caused pollution or toxicity to the environment. With the development of industry, the local enrichment of cadmium and its compounds is becoming more and more serious, causing serious pollution and poisoning to the enrichment environment, and posing a serious threat to the ecological environment, animals, plants an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/14A01N63/20A01P21/00C02F3/34C12R1/43C09K101/00C02F101/20
CPCC12N1/20C09K17/14A01N63/20C02F3/34C09K2101/00C02F2101/20
Inventor 谭泽文龚贝妮李永涛徐会娟陈恋刘怡灵
Owner SOUTH CHINA AGRI UNIV
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