Preparation of array electrode chip combined with micro-fluidic clamp and construction method of electrochemical immunosensor

Abstract

The invention relates to preparation of an array electrode chip combined with a micro-fluidic clamp and a construction method of an electrochemical immunosensor. The array electrode chip combined with the micro-fluidic clamp is characterized in that the multichannel array electrochemistry can be used for quantitative automatic sample introduction of multichannel samples, the individual difference of electrodes is small, the stability is high, electrochemical biological functional modification can be carried out, the detection volume is small, and high-flux multichannel integration is facilitated; the surface of an electrode is modified with a double-metal nano material through chemical bond action and coupled with an organophosphorus pesticide antibody, and the organophosphorus pesticide antibody can be dropwise added gradually or dropwise added in parallel by adopting a biological sample application technology, so that a complete specific immunosensor can be constructed on the surface of each working electrode. In addition, the built electrochemical immunosensor is used for realizing multi-channel detection of the organophosphorus pesticide, so that synchronous multi-channel detection of the organophosphorus pesticide is realized; and the electrode chip based on the combination of the array electrode and the micro-fluidic clamp and the electrochemical immunosensor prepared by the steps are simple to operate, high in sensitivity and better in specificity and stability, and meet the requirements of on-site rapid detection.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunosensing detection, in particular to the preparation of an array electrode chip combined with a microfluidic fixture and the construction method of an electrochemical immunosensor. Background technique [0002] Electrochemical immunosensor (Electrochemical immunosensor) is a kind of biosensor constructed by combining immunoassay and electrochemical sensing technology. It is applied to the analysis and research of trace immunogenic substances. Electrochemical immunosensor is a kind of biosensor. , is a device constructed by combining electrochemical analysis methods and immunological techniques. Electrochemical immunosensors have the advantages of strong specificity and high sensitivity, and have extremely wide applications in the fields of biology, environment, medicine, food, agriculture, etc. , the working principle of the electrochemical immunosensor is very similar to the traditio...

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Problems solved by technology

[0003] Micro-Electro-Mechanical System (MEMS, Micro-Electro-Mechanical System) is developed on the basis of microelectronics technology (semiconductor manufacturing technology), integrating lithography, corrosion, thin film, LIGA, silicon micromachining, non-silicon micromachining and precision High-tech electronic devices produced by machining and other technologies, based on MEMS devices, have the advantages of small size, high precision and good consistency. Through MEMS, the three-dimensional size of sensitive components of biosensors can be fully compressed, and the number of samples and detection speed can be improved. Therefore, it is necessary to combine electrochemical technology with MEMS to make MEMS-based electrochemical electrode arrays. At present, MEMS-based electrode arrays are mostly two-electrode systems based on silicon substrates. Due to the lack of reference electrodes for three-electrode systems leading to a decrease in the stability of the system

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