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Processing method for extracting superoxide dismutase from corn germs

A technology of superoxide and corn germ, which is applied in the methods of stress-stimulated microbial growth, oxidoreductases, biochemical equipment and methods, etc., can solve the problems of difficult extraction, purification accuracy needs to be improved, and ice cubes are difficult to dissolve and extract effectively. , to achieve the effect of improving the purification effect, the extraction range and the extraction accuracy

Inactive Publication Date: 2021-04-13
刘越伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In the existing superoxide dismutase SOD extraction technology, a method that utilizes the different freezing points in the original solution to purify at low temperature can effectively protect the activity of superoxide dismutase SOD, and the operation is simple and can effectively control the processing Cost, Chinese Invention Publication No. CN102517264B "Method for Extracting Superoxide Dismutase from Plants", the implementation scheme is as follows: the first step: the plants are pretreated to obtain plant slurry, and water-soluble copper salts or zinc salts are dissolved in the above-mentioned In the plant slurry, the slurry is heated and then cooled to obtain the heat-denatured plant slurry; the second step: the heat-denatured plant slurry obtained in the first step is directly separated from the solid-liquid, and the clear liquid obtained after the separation is frozen to become a frozen solid; The third step: rinsing the above-mentioned frozen solid, taking the solution obtained by rinsing, which is the concentrated solution of superoxide dismutase, but only using water rinsing, it is difficult to effectively dissolve and extract the inside of the ice cube, which leads to It is difficult to extract completely without dead ends, and on the other hand, the purification accuracy needs to be improved

Method used

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  • Processing method for extracting superoxide dismutase from corn germs
  • Processing method for extracting superoxide dismutase from corn germs
  • Processing method for extracting superoxide dismutase from corn germs

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Experimental program
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Effect test

Embodiment 1

[0046] see figure 1 , the processing method of extracting superoxide dismutase in corn germ, comprises the following steps:

[0047] S1, select corn germ and pulverize to generate corn germ powder, put the corn germ powder into absolute ethanol to soak and drain;

[0048] S2, adding 2% by mass active protective agent to the drained corn germ powder for wall breaking treatment to obtain germ stock solution;

[0049] S3. Dissolving zinc gluconate with a mass percentage of 45-65% of corn germ powder in the germ stock solution, and then performing heat denaturation treatment to obtain a denatured slurry. The heat denaturation temperature is 60° C. and the duration is 10 min;

[0050] S4. After the denatured slurry is cooled to 4°C, perform centrifugation treatment at 8000r / min, take the supernatant and distribute it in the scatter-point embedding device, put in the internal sensory ball 4 with a volume ratio of 40%, and then perform freezing treatment to obtain more thinned icic...

Embodiment 2

[0061] see figure 1 , the processing method of extracting superoxide dismutase in corn germ, comprises the following steps:

[0062] S1, select corn germ and pulverize to generate corn germ powder, put the corn germ powder into absolute ethanol to soak and drain;

[0063] S2, adding 3% by mass active protective agent to the drained corn germ powder for wall breaking treatment to obtain germ stock solution;

[0064] S3, dissolving zinc gluconate with a mass percentage of 55% of corn germ powder in the germ stock solution, and then performing heat denaturation treatment to obtain a denatured slurry, the heat denaturation temperature is 60°C, and the duration is 15 minutes;

[0065] S4. After the denatured slurry is cooled to 4°C, perform centrifugation treatment at 9000r / min, take the supernatant and distribute it in the scatter-point embedding device, put in the internal sensory ball 4 with a volume ratio of 50%, and then perform freezing treatment to obtain more thinned icic...

Embodiment 3

[0070] see figure 1 , the processing method of extracting superoxide dismutase in corn germ, comprises the following steps:

[0071] S1, select corn germ and pulverize to generate corn germ powder, put the corn germ powder into absolute ethanol to soak and drain;

[0072] S2, adding 5% by mass active protective agent to the drained corn germ powder for wall breaking treatment to obtain germ stock solution;

[0073] S3. Dissolving zinc gluconate with a mass percentage of 65% of corn germ powder in the germ stock solution, and then performing heat denaturation treatment to obtain a denatured slurry. The heat denaturation temperature is 60° C. and the duration is 20 min;

[0074] S4. After the denatured slurry is cooled to 4°C, perform centrifugal separation treatment at 10,000 r / min, take the supernatant and distribute it in the scatter-point embedding device, put in the internal sensory ball 4 with a volume ratio of 60%, and perform freezing treatment to obtain more thinned ici...

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Abstract

The invention discloses a processing method for extracting superoxide dismutase from corn germs, belongs to the technical field of superoxide dismutase extraction. According to the processing method, an active protecting agent is innovatively added after impurities are removed to perform effective protection on the activity of superoxide dismutase SOD, and a solution is obtained by separation after thermal denaturation; after an introduced scatter point netting device is used for uniform equal division, an inner sensing ball is put into the scatter point netting device and frozen to obtain a refined ice column, meanwhile, a netting elution column arranged in the scatter point netting device can gather ice columns to a certain degree by utilizing the metalloenzyme characteristic of superoxide dismutase before freezing, the freezing point difference is utilized after icing. the inner sensing ball automatically senses dissolution of slurry containing zinc ions and superoxide dismutase, then an ice breaking phenomenon is triggered by utilizing the magnetic attraction effect, a purification channel is opened up in an ice columns superoxide dismutase is effectively extracted. Compared with the prior art, the extraction range is undoubtedly and remarkably widened, and the extraction precision is undoubtedly and remarkably improved.

Description

technical field [0001] The invention relates to the technical field of superoxide dismutase extraction, in particular to a processing method for extracting superoxide dismutase from corn germ. Background technique [0002] Superoxide dismutase SOD is a metalloenzyme (containing copper, zinc, iron and manganese) widely present in animals and plants. It is the main antioxidant substance in the human body, and it is the nemesis and specific scavenger of oxygen free radicals . Exogenous supplementation of SOD can increase the content of SOD in the blood, decompose and eliminate excess free radicals in time, thereby protecting the normal cells and tissues of the body, avoiding the occurrence of diseases, and keeping people healthy and energetic. [0003] At present, the production of superoxide dismutase mainly comes from animal blood and liver, and the extraction of SOD from animal blood or tissue is prone to viral cross-infection. Therefore, the extraction and development of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12M1/42C12M1/00
CPCC12N9/0089C12Y115/01001C12M21/14
Inventor 刘越伟
Owner 刘越伟